Bazı Entomopatojen Fungus Kültür Filtratlarının Tetranychus urticae Koch (Acari: Tetranychidae) Üzerindeki Etkinliklerinin Belirlenmesi

Year 2023, Volume: 10 Issue: 2, 292 - 298, 25.04.2023

Asiye Uzun Yiğit , Şerife Evrim Arıcı , Ozan Demirözer

https://doi.org/10.30910/turkjans.1226848

Abstract

Bu çalışmada, 5 farklı entomopatojen fungus izolatına (Beauveria varroe, Metarhizium robertsii, Purpureocillium lilacinum, Fusarium subglutinans 8A ve F. subglutinans 12A) ait saf kültür filtratlarının Tetranychus urticae Koch’nin ergin dişileri üzerinde lethal etkileri belirlenmiştir. Plastik petrilerde (9 cm) yaprak disk yöntemine göre hazırlanmış fasulye yapraklarına (4 cm) 20 yeni ergin birey aktarılmıştır. Her bir entomopatojen fungus izolatına ait saf kültür filtratları püskürtme yöntemiyle (4 bar) 10 sn süre ile ergin bireyleri içeren yaprak disklerin üzerine uygulanmıştır. Gözlemlere uygulamadan 24 saat sonra başlanarak 7. güne kadar devam edilmiştir. Denemeler her bir entomopatojen izolatına ait uygulama dozu için 5 tekerrürlü olarak yürütülmüştür. Kültür filtratları uygulandıktan 24 ve 96 saat sonra T. urticae erginlerinde meydana gelen ölüm oranları arasında fark olmadığı bulunmuştur (P˃ 0.05). Uygulamadan 5 gün sonra ise B. varroe, F. subglutinans 8A, F. subglutinans 12A ve P. lilacinum uygulamaları arasında fark olmadığı ve M. robertsii’nin (%36±3.67) diğer entomopatojen fungus izolatlarından (%59±3.31-66±3.67) istatistiki olarak farklı olduğu saptanmıştır (P˂0.05). Son gözlem zamanı olan 7. günde, B. varroe kültür filtratının T. urticae erginleri üzerinde en yüksek ölüme (%80±3.53) neden olduğu, ayrıca F. subglutinans 12A, F. subglutinans 8A, P. lilacinum ve M. robertsii uygulamalarında belirlenen ölüm yüzdeleri ise sırasıyla %78±7.17, %76±6.00, %73±4.06, %46±2.91 olarak saptanmıştır. Metarhizium robertsii kültür filtratı uygulamasındaki T. urticae erginleri üzerindeki ölüm oranları, diğer entomopatojen fungus kültür filtratı uygulamalarından farklı bulunmuştur (P˂0.05).

Keywords

Tarımsal zararlı, Mikrobiyal mücadele, İki noktalı kırmızıörümcek

DETERMINATION OF THE EFFICACY OF ENTOMOPATHOGENIC FUNGAL CULTURE FILTRATES ON TETRANYCHUS URTICAE KOCH

Abstract

In this study, lethal effects of pure culture filtrates of 5 different isolates of entomopathogenic fungi (Beauveria varroe, Metarhizium robertsii, Purpureocillium lilacinum, Fusarium subglutinans 8A ve F. subglutinans 12A) on adult females of Tetranychus urticae Koch were determined. 20 new adult individuals were transferred to bean leaves (4 cm) prepared according to the leaf disc method in plastic petri dishes (9 cm). Pure culture filtrates of each entomopathogenic fungus isolate were applied by spraying (4 bar) on leaf discs containing adult individuals for 10 seconds. Observations were started 24 hours after the application and continued until the 7th day. Experiments were carried out with 5 replications for the application dose of each entomopathogen isolate. It was found that there was no difference between the mortality rates of T. urticae adults 24 and 96 hours after the culture filtrates were applied (P˃ 0.05). 5 days after the application, there was no difference between B. varroe, F. subglutinans 8A, F. subglutinans 12A, and P. lilacinum applications, and M. robertsii (36±3.67%) were statistically different from other entomopathogenic fungus isolates (%59±3.31-66±3.67) (P˂0.05). On the 7th day, the last observation time, it was determined that B. varroe culture filtrate caused the highest mortality (80±3.53%) on T. urticae adults, and mortality rates in F. subglutinans 12A, F. subglutinans 8A, P. lilacinum and M. robertsii applications were 78±7.17%, 76±6.0%, 73%±4.06, and 46±2.91%, respectively. Mortality rates on T. urticae adults in M. robertsii culture filtrate application were different from other entomopathogenic fungus culture filtrate applications (P˂0.05).

Keywords

Agricultural pest, Microbial control, Two-spotted spider mite

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