Pathotogenicity of Turkish Entomopathogenic nematodes, Steinernema feltiae and Steinernema carpocapsae (Rhabditida: Steinernematidae) on the rice weevil: Sitophilus oryzae (Coleoptera: Curculionidae)

Year 2023, Volume: 10 Issue: 3, 541 - 547, 23.07.2023

Alperen Kaan Bütüner , Alper Susurluk

https://doi.org/10.30910/turkjans.1295616

Cited By: 4

Abstract

Stored product pests are one of the most important biotic factors that cause serious postharvest losses of food crops in the course of storage. To control these pests, pesticides have been extensively used for many years. However, it is known that pesticides and their residues have toxic effects on non-target organisms. Therefore, it is believed that Entomopathogenic Nematodes (EPNs), which have been widely employed for biological control in agricultural fields, can be used for the potential control of Sitophilus oryzae L., 1763 (Coleoptera: Curculionidae). In this study, the biocontrol potential of Steinernema feltiae TUR-S3 and Steinernema carpocapsae TUR-S4 isolates (Rhabditida: Steinernema) were evaluated against the adults of S. oryzae at eight different concentrations [250 IJs/cm2 (600 IJs/adult), 200 IJs/cm2 (480 IJs/adult), 100 IJs/cm2 (240 IJs/adult), 50 IJs/cm2 (120 IJs/adult), 25 IJs/cm2 (60 IJs/adult), 12 IJs/cm2 (30 IJs/adult), 6 IJs/cm2 (15 IJs/adult), and 3 IJs/cm2 (7.5 IJs/adult)]. According to the results, both S. feltiae TUR-S3 and S. carpocapsae TUR-S4 exhibited the highest mortality rate (96.67%) on S. oryzae when applied at the concentration of 250 IJs/cm2. The most effective LC50 and LC90 values for S. feltiae TUR-S3 were determined as 47.55 and 167.16, respectively. These values were obtained as 35.66 and 121.79 for S. carpocapsae TUR-S4 isolate.

Keywords

Steinernema carpocapsae, Steinernema feltiae, Sitophilus oryzae, rice weevil, biological control

Supporting Institution

TÜBİTAK

Project Number

219O370

Thanks

This study was financially supported by the TUBITAK (The Scientific and Technological Research Council of Türkiye), Project number: 219O370. Prof. Dr. Özgür SAĞLAM is thanked for providing the rice weevil. Additionally, Dr. Tufan Can ULU is thanked for providing statistical support.

Türk Entomopatojen Nematodların, Steinernema feltiae ve Steinernema carpocapsae (Rhabditida: Steinernematidae) Pirinç Biti Sitophilus oryzae (Coleoptera: Curculionidae) Üzerinde Patojenisitesi

Abstract

Depolanmış ürün zararlıları, tarım ürünlerin depolama sürecinde hasat sonrası ciddi kayıplara neden olan en önemli biyotik faktörlerden biridir. Bu zararlılar ile mücadele amacıyla yıllardır yaygınlıkla pestisitler kullanılmaktadır. Ancak pestisitlerin ve ne olduğu kalıntıların hedef dışı organizmalar üzerinde oluşturduğu toksik etkiler bilinmektedir. Bu nedenle, tarım alanlarında biyolojik mücadelede yaygın olarak kullanılan Entomopatojen Nematodların (EPN), Sitophilus oryzae L., 1763 (Coleoptera: Curculionidae)'nin potansiyel kontrolünde kullanılabileceği düşünülmektedir. Bu çalışmada Steinernema feltiae TUR-S3 ve Steinernema carpocapsae TUR-S4 izolatlarının (Rhabditida: Steinernema) S. oryzae' nin erginleri üzerindeki biyokontrol potansiyel 8 farklı konsantrasyonda [250 IJs/cm2 (600 IJs/ergin), 200 IJs/cm2 (480 IJs/ergin), 100 IJs/cm2 (240 IJs/ergin), 50 IJs/cm2 (120 IJs/ergin), 25 IJs/cm2 (60 IJs/ergin), 12 IJs/cm2 (30 IJs/ergin), 6 IJs/cm2 (15 IJs/ergin), ve 3 IJs/cm2 (7.5 IJs/ergin)] değerlendirilmiştir. Sonuçlara göre hem S. feltiae TUR-S3 izolatı hemde S. carpocapsae TUR-S4 izolatı, S. oryzae erginlerinde en yüksek ölüm oranını (96.67%) 250 IJs/cm2 uygulama konsantrasyonunda gerçekleştirmiştir. S. feltiae TUR-S3 için en etkili LC50 ve LC90 değerleri sırasıyla 47.55 ve 167.16 dir. Bu değerler S. carpocapsae TUR-S4 izolatı için 35.66 ve 121.79 olarak elde edilmiştir.

Keywords

Steinernema carpocapsae, Steinernema feltiae, Sitophilus oryzae, pirinç biti, biyolojik mücadele

Project Number

219O370

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