Mycelial Mass Development of Antagonist Clonostachys rosea Isolate in Different pH Conditions

Yıl 2024, Cilt: 11 Sayı: 1, 149 - 155, 28.01.2024

Fatih Ölmez , Şahimerdan Türkölmez

https://doi.org/10.30910/turkjans.1355645

Öz

Biopesticides are one of the most popular elements of the fight against plant diseases and pests, biology. Clonostachys rosae is a mycoparasitic fungus that can act against many plant pathogenic fungi. The main obstacle to the widespread use of biological control agents is the difficulties encountered in mass production to a certain standard. Developing in solid media has disadvantages such as the relatively low amount of product obtained and the difficulty of obtaining products suitable for end use. In this study, mycelial mass growth of the antagonist fungus C. rosea in liquid culture at different pH conditions was investigated. The pH of the potato dextrose broth liquid medium was adjusted to 1.5, 2.5, 3.5, 4.5, 5.5, 6.5, 7 and 8 and sterilized at 121 °C for 20 minutes. Then, 0.5 mL C. rosea spore suspension with 1x106 spores mL-1 was added to 30 mL Erlenmeyer flasks with various pH values and incubated in a shaker incubator at 50-100 rpm for 8 days at 25 °C. As a result of the studies carried out for the mycelial growth of the antagonist C. rosea isolate, it was determined that the optimum pH level where it developed best and formed the most mycelial mass, has been determined as pH 6.5 for both wet and dry weight. In addition, C. rosea did not develop between 1.5-3.5 pH values. The data obtained are expected to contribute to the mass production of C. rosea.

Anahtar Kelimeler

Fungi, antagonist, Clonostachys rosea, mass production, biological control.

Antagonist Clonostachys rosea İzolatının Farklı Ph Koşullarında Miselial Kitle Gelişimi

Öz

Biyopestisitler, bitki hastalık ve zararlılarla mücadelede, biyoloji mücadelenin en popüler unsurlarındandırlar. Clonostachys rosae birçok bitki patojeni fungusa karşı etki gösterebilen mikoparazit bir fungustur. Biyolojik mücadele ajanlarının yaygın kullanımlarının önündeki en büyük engel, belli bir standartta kitlesel olarak üretilmelerinde karşılaşılan zorluklardır. Katı besi yerlerinde geliştirme, elde edilen ürün miktarının nispeten az olması ve son kullanıma uygun ürün eldesinin zor olması gibi olumsuzluklar içermektedir. Bu çalışma kapsamında antagonist fungus C. rosea’nin sıvı kültürde, farklı pH koşullarındaki miseliyal kitle gelişimi incelenmiştir. Patates Dekstroz Broth sıvı ortamının pH'sı 1.5, 2.5, 3.5, 4.5, 5.5, 6.5, 7 ve 8’e ayarlanmış ve 121 °C'de 20 dakika sterilize edilerek 0.5 mL C. rosea spor süspansiyonu çeşitli pH değerlerine sahip 30 mL Erlenmeyer şişelerine aşılanmıştır ve 25 ° C 'de 8 gün çalkalayıcılı inkübatörde 50-100 rpm’de inkübe edilmiştir. Antagonist C. rosea izolatının miselyal gelişimi için yapılan çalışmalar sonucunda C. rosea’nin 1,5-3,5 pH değerleri arasında gelişim göstermediği, en iyi geliştiği ve en fazla miseliyal kitle oluşturduğu optimum pH derecesinin hem yaş ve hem de kuru ağırlıkta pH 6.5 seviyesinde olduğu belirlenmiştir. Elde edilen verilerin C.rosea’nın kitlesel üretimine katkı sunması beklenmektedir.

Anahtar Kelimeler

Fungus, Clonostachys rosea, Biyolojik mücadele, Antogonist

Kaynakça

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