Soya Fasulyesi (Glycine max) Çeşitlerinin Soybean Mosaic Virus’a Dayanıklılık Durumlarının Belirlenmesi

Yıl 2024, Cilt: 11 Sayı: 4, 1200 - 1206, 12.10.2024

Abdullah Baltacı , İlyas Deligöz

https://doi.org/10.30910/turkjans.1465085

Öz

Soybean mosaic virus (SMV) soyanın en yaygın ve tahripkar viral patojenlerinden bir tanesidir. Virüsün kontrolünde dayanıklı çeşit kullanmak en etkili mücadele yöntemlerindendir. Bu çalışmada Türkiye’de tescil edilmiş olan 33 soya çeşidi 2023 yılında kontrollü koşullarda SMV’ye karşı test edilmiştir. Soya çeşitlerinin SMV’ye karşı reaksiyonlarının belirlenmesinde Samsun ilinde soya bitkisinden izole edilen SMV-STk izolatı kullanılmıştır. Her çeşide ait 10 soya bitkisi, virüs ile mekanik olarak inokule edilmiş ve inokulasyondan 21 gün sonra belirti oluşumuna göre bitkiler değerlendirilmiştir. Ayrıca bitkilerdeki virüs varlığı ya da yokluğu DAS-ELISA testi ile belirlenmiştir. Çalışma sonucunda 33 çeşitten 28 tanesi SMV ile sistemik olarak enfekteli hale gelmiş ve bu çeşitlerde DAS-ELISA (Double Antibody Sandwich Enzyme-linked Immunosorbent Assay) testi ile virüs tespit edilmiştir. Diğer yandan 5 çeşit SMV’ye karşı dayanıklı olarak bulunmuş ve dayanıklılık durumları DAS-ELISA testi ile doğrulanmıştır. Bu çalışmada SMV’ye dayanıklı olarak belirlenen çeşitler, Türkiye’de SMV’nin sorun olduğu soya üretim alanlarında önerilebilir.

Anahtar Kelimeler

Dayanıklılık, SMV, Soya, Virüs

Destekleyen Kurum

Bu çalışma, Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü, Karadeniz Tarımsal Araştırma Enstitüsü tarafından desteklenmiştir.

Teşekkür

Soya çeşitlerinin temin edilmesindeki katkılarından dolayı Seyit Ahmet EROL’a teşekkür ederiz.

Evaluation of Soybean (Glycine max) Cultivars for Resistance to Soybean Mosaic Virus

Öz

Soybean mosaic virus (SMV) is one of the most prevalent and devastating viral pathogens of soybean worldwide. The use of resistant varieties is one of the most effective ways for virus control. In this study, 33 soybean cultivars registered in Turkiye were tested to SMV under controlled conditions in 2023. A local isolate SMV-STk isolated from a soybean plant in Samsun province of Turkiye was used to assess the reaction of soybean cultivars to SMV. Ten young seedlings of each cultivar were mechanically inoculated with the virus, and the plants were evaluated according to symptomatic appearance of virus infection 21 days after inoculation. Also, virus presence or absence was confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). As a result of this study, 28 of 33 cultivars tested became systemically infected with SMV, and the virus was detected in these plants. On the other hand, 5 cultivars were found to be resistant to SMV, and their resistance was confirmed by DAS-ELISA. The resistant cultivars identified in this study can be recommended in soybean growing areas of Türkiye where SMV is a problem.

Anahtar Kelimeler

Resistance, SMV, Soybean, Virus

Kaynakça

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