Doğu Akdeniz Bölgesindeki Örtüaltı Domates Üretim Alanlarındaki Begomovirus Enfeksiyonlarının Araştırılması

Year 2025, Volume: 12 Issue: 2, 433 - 440, 16.04.2025

Selin Ceren Balsak

https://doi.org/10.30910/turkjans.1603727

Abstract

Begomovirüsler ekonomik olarak önemli bitkilerde enfeksiyon yapan virüslerin en fazla sayıda bulunan gruptur. Begomovirüslerin geniş konukçu dizinine sahip olması, yüksek rekombinasyon yeteneği, vektörüyle etkili şekilde taşınması ve konukçu bitkide farklı virüsler ile sinerjik etkileşimi nedeniyle üretim alanlarında kısa sürede geniş alanlara yayılmaları ve epidemilere yol açma riskleri oldukça yüksektir. Bu çalışmada, domates (Solanum lycopersicum L.) yetiştiriciliğini tehdit eden begomovirüslerin varlığının araştırılması amaçlanmıştır. Adana ve Kahramanmaraş illerinde ticari seralara yapılan sörveylerde sararma, kıvrılma ve bodurlaşma gibi simptom taşıyan domates bitkilerinden toplam 160 yaprak örneği toplanmıştır. Toplanan yaprak örneklerinden elde edilen total DNA’lar multipleks PCR ile testlenmiştir. Analiz sonucunda, 10 örneğin yalnızca TYLCSV ile enfekte olduğu, 16 örneğin ise TYLCSV-Sic ve TYLCV-IL ile enfekteli olduğu belirlenmiştir. Ayrıca, 22 örneğin TYLCV-IL ve TYLCV-Mld ile karışık enfekteli olduğu belirlenmiştir. Bunun yanı sıra, ToLCNDV varlığına yönelik PCR testi yapılmış, ancak hiçbir örnekte enfeksiyon tespit edilmemiştir.

Keywords

Begomovirus , TYLCV , TOLCNDV , domates , Multipleks PCR

Project Number

Project No: 2023/6-20 M

Investigation of Begomovirus Infections in Greenhouse Tomato Production Areas in the Eastern Mediterranean Region

Abstract

Begomoviruses are the most numerous group of viruses infecting economically important plants. Begomoviruses have a wide host range, high recombination ability, effective transmission by its vector and synergistic interaction with different viruses in the host plant, so they have a high risk of spreading to large areas in a short time and causing epidemics. In this study, the aim was to investigate the presence of begomoviruses, which are among the major threats to tomato (Solanum lycopersicum L.) cultivation. The surveys were conducted in the provinces of Adana and Kahramanmaraş, Turkey, where a total of 160 leaf samples were collected from tomato plants exhibiting yellowing, curling, and plant stunting in commercial greenhouses. Total DNAs obtained from the collected leaf samples were tested using multiplex PCR. As a result of the analysis, 10 samples were infected only with TYLCSV-Sic, while 16 were co-infected with TYLCSV-Sic and TYLCV-IL. Additionally, 22 of the samples were co-infected with TYLCV-IL and TYLCV-Mld. Furthermore, PCR testing for the presence of ToLCNDV was conducted, but none of the samples were found to be infected.

Keywords

Begomovirus , TYLCV , TOLCNDV , tomato , Multiplex PCR

Project Number

Project No: 2023/6-20 M

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