Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis

Esra Bulut Atalay; Hülya Ayar Kayalı

doi:10.29137/umagd.1142303

TR EN

Kolon kanseri ksenograft modellerinin farklı dokularında enerji metabolizmasının TCA ve Cori döngüleri ve Glikoliz üzerinde araştırılması

Abstract

Kanser hücrelerinde metabolizma, malign özelliklerinin gereksinimlerini karşılamak için yeniden programlanır. Bazı spesifik metabolitler, farklı kanser türlerinde malign dönüşüme katkıda bulunabilir. Bu çalışmada bir kolon adenokarsinom hücre hattı (SW480) tarafından oluşturulan ksenograft modellerinin farklı dokularında (tümör, kolon, beyin ve karaciğer) glukoz, piruvat, laktat, sitrat, alfa-ketoglutarat (α-KG), 2-hidroksiglutarat (2-HG), süksinat, fumarat, malat ve oksaloasetat düzeylerinin belirlenmesi amaçlanmıştır. Glikoz ve piruvat seviyeleri sırasıyla 3,5-dinitrosalisilik asit (DNS) yöntemi ve piruvat analizi ile belirlenmiştir. Diğer metabolitlerin seviyesi, HPLC analizi ile belirlenmiştir. Sonuçlara bakıldığında D-2-HG'nin tümör dokularında (120.2 mg/L) yüksek oranda üretildiği ancak karaciğer dokusunda tespit edilemediğini görülmektedir. Sonuç olarak, kolon kanseri ksenograft modellerinin farklı dokularında TCA ve Cori döngüleri ve Glikoliz incelenmiş ve bazı metabolitlerin dokuya özgü üretildiği bulunmuştur.

Keywords

Kolon kanseri, ksenograft model, hedeflenmiş metabolomiks, metabolit, HPLC

Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis

Abstract

The metabolism is reprogrammed in cancer cells to meet the requirement of their malignant properties. Some specific metabolites can contribute to the malignant transformation in different cancer types. This study aimed to determine the level of glucose, pyruvate, lactate, citrate, alpha-ketoglutarate (α-KG), 2-hydroxyglutarate (2-HG), succinate, fumarate, malate, and oxaloacetate in the different tissues (tumor, colon, brain, and liver) of xenograft models generated by a colon adenocarcinoma cell line (SW480). Glucose and pyruvate levels were determined by the 3,5-dinitrosalicylic acid (DNS) method and pyruvate assay, respectively. The level of other metabolites was determined by the HPLC analysis. The results show that D-2-HG is highly produced in the tumor tissues (120.2 mg/L), but it could not be detected in liver tissue. In conclusion, TCA and Cori cycles and Glycolysis were investigated in the different tissues of colon cancer xenograft models and found that some metabolites produced tissue-specific.

Keywords

Colon cancer, xenograft model, targeted metabolomics, metabolite

Supporting Institution

yok

Project Number

yok

Thanks

We gratefully thank veterinary pathologist Prof. Dr. Serap Birincioğlu (İzmir Biomedicine and Genome Center, Histopathology Unit) who evaluated histopathological findings. İzmir International Biomedicine and Genome Institute Local Ethics Committee for Animal Experiments (IBG-AELEC) approved all the conditions, protocol number: 17/2019.

References

Referans1 Sung, H., Ferlay, J., Siegel, R. L., Laversanne, M., Soerjomataram, I., Jemal, A., & Bray, F. (2021). Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 71(3), 209-249. doi:10.3322/caac.21660
Referans2 Siegel, R. L., Miller, K. D., & Jemal, A. (2019). Cancer statistics. CA: A Cancer Journal for Clinicians, 69(1), 7-34. doi: 10.3322/caac.21551

Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Esra Bulut Atalay
0000-0002-1615-0535
Türkiye

Hülya Ayar Kayalı ^*
0000-0003-0246-1866
Türkiye

Publication Date

January 31, 2023

Submission Date

July 7, 2022

Acceptance Date

July 28, 2022

Published in Issue

Year 2023 Volume: 15 Number: 1

DOI

https://doi.org/10.29137/umagd.1142303

IZ

https://izlik.org/JA97CZ76GH

APA

Bulut Atalay, E., & Ayar Kayalı, H. (2023). Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis. International Journal of Engineering Research and Development, 15(1), 117-124. https://doi.org/10.29137/umagd.1142303

AMA

1.Bulut Atalay E, Ayar Kayalı H. Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis. IJERAD. 2023;15(1):117-124. doi:10.29137/umagd.1142303

Chicago

Bulut Atalay, Esra, and Hülya Ayar Kayalı. 2023. “Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis”. International Journal of Engineering Research and Development 15 (1): 117-24. https://doi.org/10.29137/umagd.1142303.

EndNote

Bulut Atalay E, Ayar Kayalı H (January 1, 2023) Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis. International Journal of Engineering Research and Development 15 1 117–124.

IEEE

[1]E. Bulut Atalay and H. Ayar Kayalı, “Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis”, IJERAD, vol. 15, no. 1, pp. 117–124, Jan. 2023, doi: 10.29137/umagd.1142303.

ISNAD

Bulut Atalay, Esra - Ayar Kayalı, Hülya. “Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis”. International Journal of Engineering Research and Development 15/1 (January 1, 2023): 117-124. https://doi.org/10.29137/umagd.1142303.

JAMA

1.Bulut Atalay E, Ayar Kayalı H. Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis. IJERAD. 2023;15:117–124.

MLA

Bulut Atalay, Esra, and Hülya Ayar Kayalı. “Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis”. International Journal of Engineering Research and Development, vol. 15, no. 1, Jan. 2023, pp. 117-24, doi:10.29137/umagd.1142303.

Vancouver

1.Esra Bulut Atalay, Hülya Ayar Kayalı. Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis. IJERAD. 2023 Jan. 1;15(1):117-24. doi:10.29137/umagd.1142303

Cited By

Novel machine‐learning bioinformatics reveal distinct metabolic alterations for enhanced colorectal cancer diagnosis and monitoring

iMetaOmics

https://doi.org/10.1002/imo2.70003

Kırıkkale University, Faculty of Engineering and Natural Science, 71450 Yahşihan / Kırıkkale, Türkiye.

ijerad@kku.edu.tr