Synthesis, Characterization and Comparison of Antibacterial Activity of Chitosan Coated Silver and Dithiocarbamate Chitosan Coated Silver Nanoparticles

Year 2025, Volume: 17 Issue: 1, 151 - 162

Murat İnal , Selma Uslu

Abstract

Silver nanoparticles are frequently used in biomedical applications due to their antimicrobial activity, especially in the production of wound dressing materials. In this study, dithiocarbamate chitosan compound, a derivative of chitosan, was synthesised. The synthesised dithiocarbamate chitosan was characterised by scanning electron microscopy, energy dispersive X-ray spectrometry and C13-nuclear magnetic resonance spectroscopy analyses. Silver nanoparticles coated with dithiocarbamate chitosan were successfully synthesised for the first time in this study. Chitosan-coated silver nanoparticles were also synthesised for comparison. The synthesised silver nanoparticles were characterised by ultraviolet-visible region spectrophotometry, size analysis and zeta potential measurements. The antibacterial activities of the synthesised silver nanoparticles against Escherichia coli and Staphylococcus aureus bacteria were tested using liquid medium dilution method. Characterisation results show that dithiocarbamate chitosan was successfully synthesised. The sizes of silver nanoparticles coated with chitosan and dithiocarbamate chitosan were determined as 175.202.98 and 158.001.27 nm and zeta potentials as 29.704.00 and 33.400.26 mV, respectively. From the results of antibacterial activity, it was determined that the synthesised nanoparticles showed a high antibacterial activity against bacteria. As a result, it was determined that dithiocarbamate chitosan coated silver nanoparticles obtained for the first time in this study have a great potential for use in the production of wound dressing materials due to their stability and high antibacterial effect.

Keywords

Dithiocarbamate chitosan, chitosan, silver nanoparticle, wound dressing material

Ethical Statement

This study was produced from Selma Uslu's thesis titled ‘Investigation of the Use of Silver and Glycyrrhizic Acid Loaded PVA Cryogels as Wound Dressing’.

Supporting Institution

Kırıkkale University Scientific Research Projects Unit

Project Number

Bu çalışma Kırıkkale Üniversitesi Bilimsel Araştırma Projeleri tarafından 2022/047 proje numarası ile desteklenmektedir.

Thanks

Scanning electron microscopy, energy dispersive X-ray spectrometry and C13-nuclear magnetic resonance spectroscopy analyses in this study were performed by METU central research laboratory.

Kitosan kaplanmış gümüş ve ditiyokarbamat kitosan kaplanmış gümüş nanopartiküllerin sentezi, karakterizasyonu ve antibakteriyel aktivitelerinin karşılaştırılması

Abstract

Gümüş nanopartiküller yüksek yüzey-hacim oranı nedeniyle, düşük konsantrasyonda bile antimikrobiyal aktivite sergilemektedirler. Bununla birlikte, düşük maliyet, düşük sitotoksisite ve immünolojik yanıt göstermektedirler. Bu nedenle, gümüş nanopartiküller antimikrobiyal aktiviteleri sebebiyle biyomedikal uygulamalarda özellikle yara örtü malzemelerinin üretiminde sıklıkla kullanılmaktadır.
Bu çalışma da kitosanın bir türevi olan ditiyokarbamat kitosan bileşiği sentezlenmiştir. Sentezlenen ditiyokarbamat kitosan taramalı elektron mikroskobu, enerji dağılım X-ışını spektrometresi ve C13-nükleer manyetik rezonans spektroskopisi analizleri ile karakterize edilmiştir. Daha sonra ilk defa bu çalışmada ditiyokarbamat kitosan ile kaplanmış gümüş nanopartiküller başarı ile sentezlenmiştir. Ayrıca karşılaştırma için kitosan ile kaplanmış gümüş nanopartiküller sentezlenmiştir. Sentezlenen gümüş nanopartiküller ultraviyole-görünür bölge spektrofotometresi, boyut analizleri ve zeta potansiyel ölçümleri ile karakterize edilmiştir. Sentezlenen gümüş nanopartiküllerin Escherichia coli ve Staphylococcus aureus bakterilerine karşı antibakteriyel aktiviteleri sıvı kültür ortamında test edilmiştir.
Karakterizasyon sonuçları ditiyokarbamat kitosanın başarılı bir şekilde sentezlendiğini göstermektedir. Kitosan ve ditiyokarbamat kitosan ile kaplanmış gümüş nanopartiküllerin boyutları sırasıyla 175 ve 158 nm ve zeta potansiyelleri ise yaklaşık 30 mV olarak belirlenmiştir. Antibakteriyel aktivite sonuçlarından sentezlenen nanopartiküllerin bakterilere karşı yüksek bir antibakteriyel aktivite gösterdiği belirlenmiştir. Sonuç olarak ilk defa bu çalışmada elde edilen ditiyokarbamat kitosan kaplanmış gümüş nanopartiküllerin kararlılıkları ve yüksek antibakteriyel etkisi sebebiyle yara örtü malzemelerinin üretiminde kullanımı açısından büyük bir potansiyele sahip olduğu belirlenmiştir.

Keywords

Ditiyokarbamat kitosan, kitosan, gümüş nanopartikül, yara örtü malzemesi

Ethical Statement

Bu çalışma Selma Uslu’nun “Gümüş ve Gilisirizik Asit Yüklenmiş PVA Kriyojellerinin Yara Örtüsü Olarak Kullanımının İncelenmesi” isimli tez çalışmasından üretilmiştir.

Supporting Institution

Kırıkkale Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

Bu çalışma Kırıkkale Üniversitesi Bilimsel Araştırma Projeleri tarafından 2022/047 proje numarası ile desteklenmektedir.

Thanks

Çalışmada kullanılan taramalı elektron mikroskobu, enerji dağılım X-ışını spektrometresi ve C13-nükleer manyetik rezonans spektroskopisi analizleri ODTÜ merkezi araştırma laboratuvarı tarafından yapılmıştır.

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