The effects of intravenous contrast substance administration on intraocular pressure, tear amount and oxidative stress in rats

Abstract

Although iodinated radiocontrast agents, which are frequently used in radiological procedures such as indirect radiography, angiography, intravenous urography and computed tomography, are generally safe, they can cause serious side effects. In this study, it was aimed to investigate the effect of intravenous ionic high osmolar contrast agent administration on intraocular pressure, tear amount and oxidant and antioxidant parameters of eye tissue. Study groups consisted of 2 groups, Group 1 (Control) and Group 2 (Urographin), and a total of 16 Wistar albino female rats were used. On the first day of the experiment, 6 ml/kg of physiological saline was administered intravenously to the control group, and the same dose of contrast agent was administered to group 2. Intraocular pressure and tear amounts were measured at 1, 6, 12, 24 and 48 hours after intravenous administration. After measurements were made at the 48th hour of the experiment, all rats were euthanized and their eye tissues were removed. In order to reveal the oxidative damage and antioxidant activity in the eye tissue, malondialdehyde and reduced glutathione levels, catalase and glutathione peroxidase enzyme activities were measured spectrophotometrically. Schimer tear test (STT-1) strip was used for tear amount measurements, and rebound tonometer Tonovet® was used as tonometer for intraocular pressure measurements. No statistically significant difference was found between the control and urographin groups in terms of intraocular pressure and tear amounts in the measurements performed at 1,6,12,24 and 48th hours after urographin administration. It was observed that the same application significantly increased the malondialdehyde level (P<0.005) in the eye tissue. There was no significant difference between the groups in terms of reduced glutathione level and catalase and glutathione peroxidase enzyme activities in eye tissue. It was evaluated that intravenous contrast agent administration causes oxidative stress in the eye tissue and this may have a long-term ocular effect

Keywords

• Contrast agent
• eye
• oxidative stress

Project Number

Araştırmamız herhangi bir proje tarafından desteklenmemiştir.

Ratlarda intravenöz kontrast madde uygulamasının göz içi basıncı, göz yaşı miktarı ve oksidatif stres üzerine etkileri

Abstract

Endirekt radyografi, anjiyografi, intravenöz ürografi ve bilgisayarlı tomografi gibi radyolojik prosedürlerde sıklıkla kullanılmakta olan iyotlu radyo konrast ajanlar genellikle güvenli olmalarına rağmen ciddi yan etkilere sebep olabilmektedir. Bu çalışmada intravenöz iyonik yüksek ozmolar kontrast madde uygulamasının göz içi basıncı, göz yaşı miktarı ve göz dokusu oksidan ve antioksidan parametreler üzerindeki etkisinin araştırılması amaçlandı. Çalışma grupları Grup 1 (Kontrol) ve Grup 2 (Ürografin) olmak üzere 2 gruptan oluştu ve toplamda 16 adet wistar albino ırkı dişi rat kullanıldı. Denemenin ilk günü kontrol grubuna intravenöz olarak 6 ml/kg dozda serum fizyolojik, grup 2’ye ise aynı dozda kontrast madde uygulaması yapıldı. İntravenöz uygulamalardan sonraki 1, 6, 12, 24 ve 48. saatlerde göz içi basıncı ve gözyaşı miktarları ölçüldü. Denemenin 48. saatinde ölçümler yapıldıktan sonra bütün ratlar ötanazi edildi ve göz dokuları çıkarıldı. Göz dokusunda oksidatif hasar ve antioksidan aktivite durumunu ortaya koyabilmek için malondialdehit ve redükte glutatyon düzeyleri ile katalaz ve glutatyon peroksidaz enzim aktivitelerine spektrofotometrik olarak bakıldı. Göz yaşı miktarı ölçümlerinde schimer tear test (STT-1) stripi, göz içi basıncı ölçümlerinde ise tonometre olarak rebound tonometre Tonovet® kullanıldı. Ürografin uygulamasından sonraki 1, 6, 12, 24 ve 48. saatlerde yapılan ölçümlerde kontrol ve ürografin grupları arasında göz içi basıncı ve gözyaşı miktarları açısından istatistiki olarak anlamlı bir farklılık saptanmadı. Aynı uygulamanın göz dokusunda malondialdehit düzeyini (P<0,005) anlamlı şekilde arttırdığı görüldü. Göz dokusu redükte glutatyon düzeyi ile katalaz ve glutatyon peroksidaz enzim aktiviteleri açısından gruplar arasında anlamlı bir farklılık tespit edilemedi. İntarvenöz kontrast madde uygulamasının göz dokusunda oksidatif strese neden olduğu ve bunun da uzun sürede oküler etkisinin olabileceği değerlendirildi.

Keywords

• Kontrast madde
• göz
• oksidatif stres.

Supporting Institution

Araştırmamız herhangi bir kurum tarafından desteklenmemiştir.

Project Number

Araştırmamız herhangi bir proje tarafından desteklenmemiştir.

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