Research Article

Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity

Number: 2 September 24, 2026

Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity

Abstract

This study evaluates the modulatory effects of extracts obtained from Punica granatum (pomegranate) peel using solvents of different polarities on the MCF-7 breast cancer cell line. Cell viability was measured using the CCK-8 method after 24 hours of incubation with extracts at concentrations of 1 and 2μl/ml. The positive control (H₂O₂) validated the experiment by reducing viability to 6.67% (p<0.001). While no significant effect was observed at the lower concentration (1 μl/ml), the methanolic extract at the 2 μl/ml dose increased viability to 103.24%, significantly promoting proliferation compared to both the control group and its lower dose (p<0.05). This dose-dependent bimodal effect exhibited by the methanolic extract (mild inhibition at low doses and proliferation at higher doses) highlights how the biological activity of P. granatum peel is highly sensitive to the solvent and concentration used. These results suggest that the role of natural products in cancer research should be evaluated beyond simple cytotoxicity, considering their potential to modulate complex cellular responses.

Keywords

References

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Details

Primary Language

English

Subjects

Veterinary Pharmacology

Journal Section

Research Article

Publication Date

September 24, 2026

Submission Date

November 5, 2025

Acceptance Date

July 8, 2026

Published in Issue

Year 2026 Number: 2

APA
Dikel, Ç., İla, H. B., Demirkol, O., Etyemez Buyukdevecı, M., Demirkale, İ., & Meşe, R. (2026). Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity. Journal of Advances in VetBio Science and Techniques, 2, 166-170. https://doi.org/10.31797/vetbio.1817893
AMA
1.Dikel Ç, İla HB, Demirkol O, Etyemez Buyukdevecı M, Demirkale İ, Meşe R. Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity. J Ad VetBio Sci Tech. 2026;(2):166-170. doi:10.31797/vetbio.1817893
Chicago
Dikel, Çiğdem, Hasan Basri İla, Onur Demirkol, Miray Etyemez Buyukdevecı, İbrahim Demirkale, and Rumeysa Meşe. 2026. “Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity”. Journal of Advances in VetBio Science and Techniques, nos. 2: 166-70. https://doi.org/10.31797/vetbio.1817893.
EndNote
Dikel Ç, İla HB, Demirkol O, Etyemez Buyukdevecı M, Demirkale İ, Meşe R (September 1, 2026) Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity. Journal of Advances in VetBio Science and Techniques 2 166–170.
IEEE
[1]Ç. Dikel, H. B. İla, O. Demirkol, M. Etyemez Buyukdevecı, İ. Demirkale, and R. Meşe, “Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity”, J Ad VetBio Sci Tech, no. 2, pp. 166–170, Sept. 2026, doi: 10.31797/vetbio.1817893.
ISNAD
Dikel, Çiğdem - İla, Hasan Basri - Demirkol, Onur - Etyemez Buyukdevecı, Miray - Demirkale, İbrahim - Meşe, Rumeysa. “Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity”. Journal of Advances in VetBio Science and Techniques. 2 (September 1, 2026): 166-170. https://doi.org/10.31797/vetbio.1817893.
JAMA
1.Dikel Ç, İla HB, Demirkol O, Etyemez Buyukdevecı M, Demirkale İ, Meşe R. Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity. J Ad VetBio Sci Tech. 2026;:166–170.
MLA
Dikel, Çiğdem, et al. “Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity”. Journal of Advances in VetBio Science and Techniques, no. 2, Sept. 2026, pp. 166-70, doi:10.31797/vetbio.1817893.
Vancouver
1.Çiğdem Dikel, Hasan Basri İla, Onur Demirkol, Miray Etyemez Buyukdevecı, İbrahim Demirkale, Rumeysa Meşe. Dose-Dependent Bimodal Effect of Pomegranate Peel Extracts on MCF-7 Cells: The Role of Solvent Polarity. J Ad VetBio Sci Tech. 2026 Sep. 1;(2):166-70. doi:10.31797/vetbio.1817893

 

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