Effect of caffeic acid phenyl ester (CAPE) on spermatological parametersin short-term storage of ram semen

Murat Kırıkkulak; Fatih Avdatek; Deniz Yeni; Mustafa Gündoğan; Ömer Hazman

doi:10.31797/vetbio.1835846

Effect of caffeic acid phenyl ester (CAPE) on spermatological parametersin short-term storage of ram semen

Abstract

This study was conducted to investigate the effects of caffeic acid phenyl ester (CAPE), one of the biologically active compo-nents of bee propolis, on spermatological parameters during short-term storage (up to 72 hours at +4 °C) of ram semen. Eja-culates from five Merino rams were mixed and divided into four groups: control, 10 μg/ml, 50 μg/ml, and 100 μg/ml CAPEtreatment groups. Sperm samples were evaluated for motility, abnormal spermatozoon rate, and plasma membrane integrity(HOS test) at 0, 24, 48, and 72 hours. The results revealed that CAPE contributed to the preservation of semen quality, especi-ally at the 100 μg/ml dose. According to the study findings, while no significant differences were found between the groups interms of motility and abnormal spermatozoon rates at 0, 24, and 48 hours, increases in plasma membrane integrity (HOS test),particularly in the 100 μg/ml group, were found to be statistically significant. At 72 hours, the significant increases in motilityvalues and decreases in abnormal spermatozoon rates in the 10 and 100 μg/ml groups indicate that CAPE supports sperm cellmembrane stabilization during short-term storage. The lack of any studies using CAPE for short-term storage of ram semenmakes this research a valuable contribution to the field. Evaluating CAPE at different doses, in relation to longer storage timesand fertilization rates, will contribute to a better understanding of its potential applications in reproductive biotechnology.

Keywords

Supporting Institution

This study was supported by the Afyon Kocatepe University Scientific Research Projects Coordination Unit under project number 18.KARİYER.133.

Project Number

18. KARİYER.133

Ethical Statement

Institution taken from: Afyon Kocatepe University Local Ethics Committee for Animal Experiments (AKÜHADYEK) Date: 13.03.2018 Number: Decision reference number 35-18.

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Details

Primary Language

English

Subjects

Reproduction and Artificial Insemination

Journal Section

Research Article

Authors

Murat Kırıkkulak ^*
0000-0002-5574-0394
Türkiye

Fatih Avdatek
0000-0003-2345-8826
Türkiye

Deniz Yeni
0000-0002-9105-5677
Türkiye

Mustafa Gündoğan
0000-0002-3292-4625
Türkiye

Ömer Hazman
0000-0002-2702-6847
Türkiye

Publication Date

May 6, 2026

Submission Date

December 4, 2025

Acceptance Date

March 8, 2026

Published in Issue

Year 2026 Number: 2

DOI

https://doi.org/10.31797/vetbio.1835846

IZ

https://izlik.org/JA26ZK45RP

Cite

RIS / Bibtex

APA

Kırıkkulak, M., Avdatek, F., Yeni, D., Gündoğan, M., & Hazman, Ö. (2026). Effect of caffeic acid phenyl ester (CAPE) on spermatological parametersin short-term storage of ram semen. Journal of Advances in VetBio Science and Techniques, 2, 70-75. https://doi.org/10.31797/vetbio.1835846

AMA

1.Kırıkkulak M, Avdatek F, Yeni D, Gündoğan M, Hazman Ö. Effect of caffeic acid phenyl ester (CAPE) on spermatological parametersin short-term storage of ram semen. J Ad VetBio Sci Tech. 2026;(2):70-75. doi:10.31797/vetbio.1835846

Chicago

Kırıkkulak, Murat, Fatih Avdatek, Deniz Yeni, Mustafa Gündoğan, and Ömer Hazman. 2026. “Effect of Caffeic Acid Phenyl Ester (CAPE) on Spermatological Parametersin Short-Term Storage of Ram Semen”. Journal of Advances in VetBio Science and Techniques, nos. 2: 70-75. https://doi.org/10.31797/vetbio.1835846.

EndNote

Kırıkkulak M, Avdatek F, Yeni D, Gündoğan M, Hazman Ö (May 1, 2026) Effect of caffeic acid phenyl ester (CAPE) on spermatological parametersin short-term storage of ram semen. Journal of Advances in VetBio Science and Techniques 2 70–75.

IEEE

[1]M. Kırıkkulak, F. Avdatek, D. Yeni, M. Gündoğan, and Ö. Hazman, “Effect of caffeic acid phenyl ester (CAPE) on spermatological parametersin short-term storage of ram semen”, J Ad VetBio Sci Tech, no. 2, pp. 70–75, May 2026, doi: 10.31797/vetbio.1835846.

ISNAD

Kırıkkulak, Murat - Avdatek, Fatih - Yeni, Deniz - Gündoğan, Mustafa - Hazman, Ömer. “Effect of Caffeic Acid Phenyl Ester (CAPE) on Spermatological Parametersin Short-Term Storage of Ram Semen”. Journal of Advances in VetBio Science and Techniques. 2 (May 1, 2026): 70-75. https://doi.org/10.31797/vetbio.1835846.

JAMA

1.Kırıkkulak M, Avdatek F, Yeni D, Gündoğan M, Hazman Ö. Effect of caffeic acid phenyl ester (CAPE) on spermatological parametersin short-term storage of ram semen. J Ad VetBio Sci Tech. 2026;:70–75.

MLA

Kırıkkulak, Murat, et al. “Effect of Caffeic Acid Phenyl Ester (CAPE) on Spermatological Parametersin Short-Term Storage of Ram Semen”. Journal of Advances in VetBio Science and Techniques, no. 2, May 2026, pp. 70-75, doi:10.31797/vetbio.1835846.

Vancouver

1.Murat Kırıkkulak, Fatih Avdatek, Deniz Yeni, Mustafa Gündoğan, Ömer Hazman. Effect of caffeic acid phenyl ester (CAPE) on spermatological parametersin short-term storage of ram semen. J Ad VetBio Sci Tech. 2026 May 1;(2):70-5. doi:10.31797/vetbio.1835846