Research Article

Effect of follicle aspiration or GnRH treatment prior to superstimulation on superovulation response in PRID treated cows absence corpus luteum

Number: 2 July 12, 2026

Effect of follicle aspiration or GnRH treatment prior to superstimulation on superovulation response in PRID treated cows absence corpus luteum

Abstract

In recent years, improvements in superovulation protocols and techniques and the determination of anti-Müllerian hormone (AMH) levels before superovulation applications in donor selection have increased the yield of transferable embryos obtained per unit time. The main objectives of this study were to compare the effect of two different follicular wave synchronization methods (GnRH and dominant follicle aspiration-DFA) applied on the same superovulation protocol timeline on superovulation response and to investigate the effect of superstimulation application on the level of AMH. A total of 30 cows were subjected to superovulation, consisting of two pre-synchronization (P) and one control (C) group, each containing 10 Holstein cows. Groups were formed as P-GnRH, P-DFA and C-GnRH. Blood samples were collected immediately before FSH injections (AMH-1) and 24 hours after superstimulation (AMH-2). The results showed that superovulation responses were higher in the presynchronized groups compared to the control (P<0.05). There was no difference in AMH-1 levels among the groups. However, AMH-2 levels increased significantly in all groups (P<0.01). In conclusion, it has been shown in cows that induction of ovulation of follicles with prolonged dominance by GnRH in the absence of CL or initiation of superstimulation after DFA results in similar embryo yields. In addition, circulating AMH levels are significantly increased after superstimulation treatments.

Keywords

Supporting Institution

This study was produced from the first author's PhD thesis. This thesis was supported by the General Directorate of Agricultural Research and Policies as a project numbered TAGEM/ HAYSÜD/ T1/ 23/ A5/ P3/6358.

Project Number

TAGEM/ HAYSÜD/ T1/ 23/ A5/ P3/6358

Ethical Statement

All procedures were carried out with the approval of the Local Ethics Committee for Animal Experiments at the Eastern Mediterranean Agricultural Research Institute. Date: 30.01.2023 Approval number: 2023/3

Thanks

We would like to thank Assoc. Prof. Dr. Hatice HIZLI who contributed to this study with her statistical analysis.

References

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Details

Primary Language

English

Subjects

Reproduction and Artificial Insemination

Journal Section

Research Article

Publication Date

July 12, 2026

Submission Date

January 16, 2026

Acceptance Date

May 9, 2026

Published in Issue

Year 2026 Number: 2

APA
Mutlu, H., & Yıldız, C. (2026). Effect of follicle aspiration or GnRH treatment prior to superstimulation on superovulation response in PRID treated cows absence corpus luteum. Journal of Advances in VetBio Science and Techniques, 2, 129-138. https://doi.org/10.31797/vetbio.1865007
AMA
1.Mutlu H, Yıldız C. Effect of follicle aspiration or GnRH treatment prior to superstimulation on superovulation response in PRID treated cows absence corpus luteum. J Ad VetBio Sci Tech. 2026;(2):129-138. doi:10.31797/vetbio.1865007
Chicago
Mutlu, Hasan, and Cengiz Yıldız. 2026. “Effect of Follicle Aspiration or GnRH Treatment Prior to Superstimulation on Superovulation Response in PRID Treated Cows Absence Corpus Luteum”. Journal of Advances in VetBio Science and Techniques, nos. 2: 129-38. https://doi.org/10.31797/vetbio.1865007.
EndNote
Mutlu H, Yıldız C (July 1, 2026) Effect of follicle aspiration or GnRH treatment prior to superstimulation on superovulation response in PRID treated cows absence corpus luteum. Journal of Advances in VetBio Science and Techniques 2 129–138.
IEEE
[1]H. Mutlu and C. Yıldız, “Effect of follicle aspiration or GnRH treatment prior to superstimulation on superovulation response in PRID treated cows absence corpus luteum”, J Ad VetBio Sci Tech, no. 2, pp. 129–138, July 2026, doi: 10.31797/vetbio.1865007.
ISNAD
Mutlu, Hasan - Yıldız, Cengiz. “Effect of Follicle Aspiration or GnRH Treatment Prior to Superstimulation on Superovulation Response in PRID Treated Cows Absence Corpus Luteum”. Journal of Advances in VetBio Science and Techniques. 2 (July 1, 2026): 129-138. https://doi.org/10.31797/vetbio.1865007.
JAMA
1.Mutlu H, Yıldız C. Effect of follicle aspiration or GnRH treatment prior to superstimulation on superovulation response in PRID treated cows absence corpus luteum. J Ad VetBio Sci Tech. 2026;:129–138.
MLA
Mutlu, Hasan, and Cengiz Yıldız. “Effect of Follicle Aspiration or GnRH Treatment Prior to Superstimulation on Superovulation Response in PRID Treated Cows Absence Corpus Luteum”. Journal of Advances in VetBio Science and Techniques, no. 2, July 2026, pp. 129-38, doi:10.31797/vetbio.1865007.
Vancouver
1.Hasan Mutlu, Cengiz Yıldız. Effect of follicle aspiration or GnRH treatment prior to superstimulation on superovulation response in PRID treated cows absence corpus luteum. J Ad VetBio Sci Tech. 2026 Jul. 1;(2):129-38. doi:10.31797/vetbio.1865007

 

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