Research Article

Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever

Number: 2 July 13, 2026

Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever

Abstract

Bovine ephemeral fever (BEF), also known as three-day sickness, is a vector-borne viral disease of cattle. It causes significant economic losses due to high fever and a sudden decline in milk yield and is characterized by low mortality but high morbidity (80–100%). The aim of this study was to determine the distribution of acute clinical findings in naturally infected Holstein and Simmental cattle with BEF, to evaluate breed-related differences, and to identify subcutaneous emphysema. A total of 32 PCR-confirmed cattle were evaluated. Vital parameters and clinical findings were assessed and recorded within the first 12 hours after the onset of clinical signs. The distribution of clinical findings was as follows (n=32): Appetite was affected in all animals, with complete anorexia observed in 75% of cases; jugular vein distension, recumbency, tremor and salivation (34.4%); lameness (31.2%); mastitis and complete loss of milk production (12.5%); constipation (6.25%); and subcutaneous emphysema (3.1%). No statistically significant difference was found in the distribution of clinical findings between Holstein and Simmental breeds. Subcutaneous emphysema had the lowest incidence among the observed clinical findings. Additionally, jugular vein distension, a clinical finding not commonly described in the literature, was recorded in both breeds during the acute phase of BEF. The combined evaluation of the clinical findings identified in this study may facilitate the diagnosis of BEF, particularly under field conditions, increase clinical suspicion, and support the early initiation of treatment protocols. Additionally, complications such as jugular vein distension, which has not been widely emphasized in previous studies, and subcutaneous emphysema, which is less common, have been observed and should be considered in clinical evaluation.

Keywords

Project Number

None

Ethical Statement

This study was conducted following the approval of the Local Animal Experiments Ethics Committee of Harran University (HRÜ-HADYEK/2025/009/06), Şanlıurfa, Türkiye.

References

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  2. Akakpo, A. J. (2015). Three-day fever. Revue Scientifique et Technique (Office International des Epizooties), 34(2), 533–538, 525–532. https://doi.org/10.20506/rst.34.2.2377
  3. Amin, A. (2016). Histopathological and immunohistochemical studies on bovine ephemeral fever in cattle. Journal of Advanced Veterinary Research, 6(3), 93–100.
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  5. Ashraf, R., Rashid, S., Riaz, M., Bajwa, M. S., Jamil, A., & Khalid, M. T. (2023). Bovine ephemeral fever (BEF). In One Health Triad (Vol. 1, pp. 134–142). Unique Scientific Publishers. https://doi.org/10.47278/book.oht/2023.21
  6. Ayvazoğlu, C., & Ayvazoğlu Demir, P. (2022). Bovine ephemeral fever in Turkey and its economic effect. Van Veterinary Journal, 33(3), 71–75. https://doi.org/10.36483/vanvetj.1141040
  7. Buczinski, S., Francoz, D., Fecteau, G., & Difruscia, R. (2010). Heart disease in cattle with clinical signs of heart failure: 59 cases. Canadian Veterinary Journal, 51(10), 1123–1129.
  8. Çamkerten, G., Çamkerten, I., Ün, H., Balkan, B. M., Ipek, H., Taskin, A., & Sahin, T. (2019). Oxidant-antioxidant status in bovine ephemeral fever. Indian Journal of Animal Research, 53(3), 408–411.

Details

Primary Language

English

Subjects

Veterinary Internal Medicine

Journal Section

Research Article

Publication Date

July 13, 2026

Submission Date

March 12, 2026

Acceptance Date

June 15, 2026

Published in Issue

Year 2026 Number: 2

APA
Şahan, A., Gülersoy, E., Dinçer, E., Balıkçı, C., Günal, İ., & Kısmet, E. (2026). Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever. Journal of Advances in VetBio Science and Techniques, 2, 139-146. https://doi.org/10.31797/vetbio.1908558
AMA
1.Şahan A, Gülersoy E, Dinçer E, Balıkçı C, Günal İ, Kısmet E. Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever. J Ad VetBio Sci Tech. 2026;(2):139-146. doi:10.31797/vetbio.1908558
Chicago
Şahan, Adem, Erdem Gülersoy, Ender Dinçer, Canberk Balıkçı, İsmail Günal, and Esma Kısmet. 2026. “Distribution of Clinical Findings and Evaluation of Infrequent Subcutaneous Emphysema in Naturally Infected Simmental and Holstein Cattle With Bovine Ephemeral Fever”. Journal of Advances in VetBio Science and Techniques, nos. 2: 139-46. https://doi.org/10.31797/vetbio.1908558.
EndNote
Şahan A, Gülersoy E, Dinçer E, Balıkçı C, Günal İ, Kısmet E (July 1, 2026) Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever. Journal of Advances in VetBio Science and Techniques 2 139–146.
IEEE
[1]A. Şahan, E. Gülersoy, E. Dinçer, C. Balıkçı, İ. Günal, and E. Kısmet, “Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever”, J Ad VetBio Sci Tech, no. 2, pp. 139–146, July 2026, doi: 10.31797/vetbio.1908558.
ISNAD
Şahan, Adem - Gülersoy, Erdem - Dinçer, Ender - Balıkçı, Canberk - Günal, İsmail - Kısmet, Esma. “Distribution of Clinical Findings and Evaluation of Infrequent Subcutaneous Emphysema in Naturally Infected Simmental and Holstein Cattle With Bovine Ephemeral Fever”. Journal of Advances in VetBio Science and Techniques. 2 (July 1, 2026): 139-146. https://doi.org/10.31797/vetbio.1908558.
JAMA
1.Şahan A, Gülersoy E, Dinçer E, Balıkçı C, Günal İ, Kısmet E. Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever. J Ad VetBio Sci Tech. 2026;:139–146.
MLA
Şahan, Adem, et al. “Distribution of Clinical Findings and Evaluation of Infrequent Subcutaneous Emphysema in Naturally Infected Simmental and Holstein Cattle With Bovine Ephemeral Fever”. Journal of Advances in VetBio Science and Techniques, no. 2, July 2026, pp. 139-46, doi:10.31797/vetbio.1908558.
Vancouver
1.Adem Şahan, Erdem Gülersoy, Ender Dinçer, Canberk Balıkçı, İsmail Günal, Esma Kısmet. Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever. J Ad VetBio Sci Tech. 2026 Jul. 1;(2):139-46. doi:10.31797/vetbio.1908558

 

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