Research Article

The role of chrysin on oxidative stress and the asymmetric dimethylarginine/ dimethylarginine dimethylaminohydrolase-1/nitric oxide synthase signaling pathway in an experimental hyperthyroidism model

Number: 2 October 8, 2026

The role of chrysin on oxidative stress and the asymmetric dimethylarginine/ dimethylarginine dimethylaminohydrolase-1/nitric oxide synthase signaling pathway in an experimental hyperthyroidism model

Abstract

The present study aimed to investigate the effects of chrysin on oxidative stress parameters and the ADMA/DDAH-1/NOS signaling pathway in rats with experimentally induced hyperthyroidism. In this study, 10–12-week-old male Sprague–Daw ley rats were used and randomly allocated into five groups: control, solvent, chrysin, hyperthyroid, and hyperthyroid + chrysin. Hyperthyroidism was induced by administration of L-thyroxine (0.3 mg/kg/day, i.p.) for eight weeks. Chrysin was administered orally by gavage at dose of 30 mg/kg/day. At the end of the experiment, body and heart weights were record ed, and serum fT3, fT4, and TSH levels were measured. In addition, ADMA, DDAH-1, and eNOS levels, as well as MDA, GSH, CAT, and GSH-Px levels in heart tissue were determined. In the hyperthyroid group, heart weight (p<0.001), serum fT3 (p<0.001) and fT4 (p<0.001) levels, and heart tissue MDA (p<0.01), ADMA (p<0.01), and eNOS (p<0.05) levels were significantly increased, whereas body weight (p<0.001), TSH (p<0.01), GSH (p<0.001), CAT (p<0.05), GSH-Px (p<0.01), and DDAH-1 (p<0.001) levels were significantly decreased. In the hyperthyroid + chrysin group, compared with the hyper thyroid group, heart weight (p<0.001), serum fT3 (p<0.01) and fT4 (p<0.001) levels, as well as heart tissue MDA (p<0.01) and eNOS (p<0.05) levels were decreased, while GSH-Px activity (p<0.01) was increased. Conclusion, hyperthyroidism induced increased oxidative stress in cardiac tissue and disrupted the ADMA/DDAH-1/NOS balance. Chrysin partially ameliorated these adverse alterations through its antioxidant effects and regulatory role in nitric oxide metabolism. These findings suggest that chrysin may represent a potential protective agent against hyperthyroidism-induced cardiac damage.

Keywords

Supporting Institution

Financial support for this research was provided by the Scientific and Technological Research Council of Türkiye (TÜBİTAK) via the 2209 Student Projects program (Project No: 1919B012323748).

Project Number

Scientific and Technological Research Council of Türkiye (TÜBİTAK) via the 2209 Student Projects program (Project No: 1919B012323748).

Ethical Statement

This study was approved by the Local Ethics Committee for Animal Experiments of Fırat University (Date: 11.09.2024, Protocol No: 2024/15-06).

References

  1. Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121-126. https://doi.org/10.1016/S0076-6879(84)05016 3.
  2. Al-Nuzaily, M., Tash, F., Farid, M., Al-Habori, M., Bosh nak, H., & Abdelaal, H. (2017). Asymmetric dimethy larginine (Adma): a novel marker of endothelial dysfunc tion in hyperthyroidism. Journal of Blood Disorders , 4(1), 1043. https://doi.org/10.1161/01.cir.98.18.1842.
  3. Araujo, A., Ribeiro, M., Enzveiler, A., Schenkel, P., Fer nandes, T., Partata, W., Irigoyen, M., Llesuy, S., & Belló-Klein, A. (2006). Myocardial antioxidant enzyme activities and concentration and glutathione metabo lism in experimental hyperthyroidism. Molecular and Cellular Endocrinology, 249(1-2), 133-139. https://doi. org/10.1016/j.mce.2006.02.005.
  4. Ateşşahin, A., Karahan, İ., Yılmaz, S., Çeribaşı, A. O., & Bulmuş, Ö. (2006). Lycopene prevents adriamycin-in duced testicular toxicity in rats. Fertility and Sterili ty, 85(1), 1216-1222. https://doi.org/10.1016/j.fertn stert.2005.11.035.
  5. Baltaci, A. (2018). Both experimental hypothyroidism and hyperthyroidism increase cardiac irisin levels in rats. Bratislava Medical Journal, 119(1), 32-35. https://doi. org/10.4149/BLL_2018_007.
  6. Barreto-Chaves, M. L. M., Senger, N., Fevereiro, M. R., Parletta, A. C., & Takano, A. P. C. (2020). Impact of hyperthyroidism on cardiac hypertrophy. Endocrine Con nections, 9(3), R59-R69. https://doi.org/10.1530/EC-19 0543.
  7. Boccalandro, C., Boccalandro, F., Orlander, P., & Wei, C. F. (2003). Severe reversible dilated cardiomyopathy and hyperthyroidism: case report and review of the lit erature. Endocrine Practice, 9(2), 140-146. https://doi. org/10.4158/EP.9.2.140.
  8. Böger, R. H. (2005). Asymmetric dimethylarginine (ADMA) and cardiovascular disease: insights from prospective clinical trials. Vascular Medicine, 10(1_suppl), S19-S25. https://doi.org/10.1177/1358836X0501000104 .

Details

Primary Language

English

Subjects

Veterinary Anatomy and Physiology

Journal Section

Research Article

Publication Date

October 8, 2026

Submission Date

April 24, 2026

Acceptance Date

September 7, 2026

Published in Issue

Year 2026 Number: 2

APA
Arkalı, G., Çakmak, R., Oray, M., Duran, B. N., Koçyiğit, F. B., & Şener, M. K. (2026). The role of chrysin on oxidative stress and the asymmetric dimethylarginine/ dimethylarginine dimethylaminohydrolase-1/nitric oxide synthase signaling pathway in an experimental hyperthyroidism model. Journal of Advances in VetBio Science and Techniques, 2, 228-234. https://doi.org/10.31797/vetbio.1926049
AMA
1.Arkalı G, Çakmak R, Oray M, Duran BN, Koçyiğit FB, Şener MK. The role of chrysin on oxidative stress and the asymmetric dimethylarginine/ dimethylarginine dimethylaminohydrolase-1/nitric oxide synthase signaling pathway in an experimental hyperthyroidism model. J Ad VetBio Sci Tech. 2026;(2):228-234. doi:10.31797/vetbio.1926049
Chicago
Arkalı, Gözde, Rana Çakmak, Merve Oray, Beyza Nur Duran, Fatma Beril Koçyiğit, and Muhammed Kerem Şener. 2026. “The Role of Chrysin on Oxidative Stress and the Asymmetric Dimethylarginine Dimethylarginine Dimethylaminohydrolase-1 Nitric Oxide Synthase Signaling Pathway in an Experimental Hyperthyroidism Model”. Journal of Advances in VetBio Science and Techniques, nos. 2: 228-34. https://doi.org/10.31797/vetbio.1926049.
EndNote
Arkalı G, Çakmak R, Oray M, Duran BN, Koçyiğit FB, Şener MK (October 1, 2026) The role of chrysin on oxidative stress and the asymmetric dimethylarginine/ dimethylarginine dimethylaminohydrolase-1/nitric oxide synthase signaling pathway in an experimental hyperthyroidism model. Journal of Advances in VetBio Science and Techniques 2 228–234.
IEEE
[1]G. Arkalı, R. Çakmak, M. Oray, B. N. Duran, F. B. Koçyiğit, and M. K. Şener, “The role of chrysin on oxidative stress and the asymmetric dimethylarginine/ dimethylarginine dimethylaminohydrolase-1/nitric oxide synthase signaling pathway in an experimental hyperthyroidism model”, J Ad VetBio Sci Tech, no. 2, pp. 228–234, Oct. 2026, doi: 10.31797/vetbio.1926049.
ISNAD
Arkalı, Gözde - Çakmak, Rana - Oray, Merve - Duran, Beyza Nur - Koçyiğit, Fatma Beril - Şener, Muhammed Kerem. “The Role of Chrysin on Oxidative Stress and the Asymmetric Dimethylarginine Dimethylarginine Dimethylaminohydrolase-1 Nitric Oxide Synthase Signaling Pathway in an Experimental Hyperthyroidism Model”. Journal of Advances in VetBio Science and Techniques. 2 (October 1, 2026): 228-234. https://doi.org/10.31797/vetbio.1926049.
JAMA
1.Arkalı G, Çakmak R, Oray M, Duran BN, Koçyiğit FB, Şener MK. The role of chrysin on oxidative stress and the asymmetric dimethylarginine/ dimethylarginine dimethylaminohydrolase-1/nitric oxide synthase signaling pathway in an experimental hyperthyroidism model. J Ad VetBio Sci Tech. 2026;:228–234.
MLA
Arkalı, Gözde, et al. “The Role of Chrysin on Oxidative Stress and the Asymmetric Dimethylarginine Dimethylarginine Dimethylaminohydrolase-1 Nitric Oxide Synthase Signaling Pathway in an Experimental Hyperthyroidism Model”. Journal of Advances in VetBio Science and Techniques, no. 2, Oct. 2026, pp. 228-34, doi:10.31797/vetbio.1926049.
Vancouver
1.Gözde Arkalı, Rana Çakmak, Merve Oray, Beyza Nur Duran, Fatma Beril Koçyiğit, Muhammed Kerem Şener. The role of chrysin on oxidative stress and the asymmetric dimethylarginine/ dimethylarginine dimethylaminohydrolase-1/nitric oxide synthase signaling pathway in an experimental hyperthyroidism model. J Ad VetBio Sci Tech. 2026 Oct. 1;(2):228-34. doi:10.31797/vetbio.1926049

 

22563     logo_world_of_journals_no_margin.png     logo.png         download?token=eyJhdXRoX3JvbGVzIjpbXSwiZW5kcG9pbnQiOiJqb3VybmFsIiwib3JpZ2luYWxuYW1lIjoiZ29vZ2xlLXNjaG9sYXIyLmpwZyIsInBhdGgiOiI0YmNjL2M3YTEvZDJlZC82YTRkMjM4ZmIzNzNlOC42ODEzNzAwNi5qcGciLCJleHAiOjE3ODM0NDM4NzEsIm5vbmNlIjoiZTZiODBhZWQ3YTNjMzYzYmJhNjhiODFkYzZiODUwMTYifQ.2JojxvY-UGBIyhQ5h9ld027SWlgZMpsDO3IfZeyd2DE