Research Article

Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index

Number: 3 October 22, 2026

Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index

Abstract

This study aimed to evaluate cyclophosphamide-induced toxicity in rats by assessing levels of catalase (CAT), nitric oxide (NO), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI). A total of 18 female Wistar albino rats were divided into three groups, with six rats in each group. The groups established were: a healthy control group (C, n=6), an ovariectomized group (O, n=6), and a group that received a single intraperitoneal (IP) dose of 200 mg/kg cyclophosphamide following ovariectomy (O+CPA, n=6). At the end of the study (24 hours after cyclophosphamide administration), the rats were euthanized via cervical dislocation under anesthesia in accordance with ethical guidelines, and blood samples were collected. Biochemical measurements (Catalase, Nitric Oxide, Total Antioxidant, and Total Oxidant) were performed on the collected blood samples. Analysis of the TAS, TOS, and OSI results revealed that TAS levels decreased, while TOS and OSI levels increased in the O+CPA group. In the O+CPA group, CAT levels decreased, whereas NO levels increased significantly. In conclusion, it has been determined that cyclophosphamide administration increases oxidative stress and impairs antioxidant defense, as evidenced by decreases in TAS and CAT levels and increases in TOS, OSI, and NO levels.

Keywords

References

  1. Abdelkawi, S. A., & Hassan, A. A. (2012). Evaluation of protein change and oxidative stress index after photodynamic therapy of corneal neovascularization. General Physiology and Biophysics, 31(04), 449–455. https://doi.org/10.4149/gpb_2012_045
  2. Abdulhussein Al-Salih, H., Mohammed Al-Sharafi, N., Sattar Al-Qabi, S., Adnan Al-Darwesh, A., & Abdulhussein Al -Salih, H. (2020). The pathological features of cyclophosphamide induced multi-organs toxicity in male Wister rats. Systematic Reviews in Pharmacy, 11(06), 2020. https://doi.org/10.31838/srp.2020.6.10
  3. Aebi, H. (1984). Catalase in vitro. Methods in enzymology, 105, 121–126. https://doi.org/10.1016/s0076-6879(84)05016-3
  4. Aslankoç, R., Demirci, D., İnan, Ü., Yıldız, M., Öztürk, A., Çetin, M., Savran, E. Ş., & Yılmaz, B. (2019). Oksidatif stres durumunda antioksidan enzimlerin rolü - Süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GPX). SDÜ Tıp Fakültesi Dergisi, 26(3), 362–369. https://doi.org/10.17343/sdutfd.566969
  5. Barnett, S., Errington, J., Sludden, J., Jamieson, D., Poinsignon, V., Paci, A., & Veal, G. J. (2021). Pharmacokinetics and pharmacogenetics of cyclophosphamide in a neonate and ınfant childhood cancer patient population. Pharmaceuticals, 14(3), 272. https://doi.org/10.3390/ph14030272
  6. Caneguim, B. H., Serpeloni, J. M., Medeiros Maciel, M. A., Mara, I., Cólus, S., De Fátima, S., & Mesquita, P. (2011). Reduction of DNA-damage by Croton cajucara methanolic extract but not the testicular alterations induced by doxorubicin. Journal of Medicinal Plants Research, 5(14), 3277–3285. http://www.academicjournals.org/JMPR
  7. Cengiz, M., Gür, B., Gür, F., Şahintürk, V., Bayrakdar, A., Şahin, I. K., Başkoy, S. A., Bilici, N., Onur, S., Kaya, Y., Kıran, İ., Yıldırım, Ö., Akkaya, N. B., Sezer, C. V., & Ayhanci, A. (2024). The protective effects of selenium and boron on cyclophosphamide-induced hepatic oxidative stress, inflammation, and apoptosis in rats. Heliyon, 10(19), e38713. https://doi.org/10.1016/j.heliyon.2024.e38713
  8. Çetinavcı, D., Yenilmez, E., Bıyık, A. F., Alver, A., & Sağlam, N. (2023). Effects of rosmarinic acid on cyclophosphamide-induced nephrotoxicity in rats. Cukurova Medical Journal, 48(3), 902–910. https://doi.org/10.17826/cumj.1317508

Details

Primary Language

English

Subjects

Veterinary Biochemistry

Journal Section

Research Article

Publication Date

October 22, 2026

Submission Date

October 3, 2026

Acceptance Date

October 20, 2026

Published in Issue

Year 2026 Number: 3

APA
Nogay, S., Dolanbay, T., Gezer, T., Demir, M. S., Demir, B., Sezer, M., & Ayyıldız Akın, M. (2026). Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index. Journal of Advances in VetBio Science and Techniques, 3, 314-318. https://doi.org/10.31797/vetbio.2054243
AMA
1.Nogay S, Dolanbay T, Gezer T, et al. Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index. J Ad VetBio Sci Tech. 2026;(3):314-318. doi:10.31797/vetbio.2054243
Chicago
Nogay, Süleyman, Turgut Dolanbay, Tahir Gezer, et al. 2026. “Evaluation of Cyclophosphamide-Induced Toxicity in Rats in Terms of Catalase, Nitric Oxide, Total Antioxidant and Total Oxidant Status, and Oxidative Stress Index”. Journal of Advances in VetBio Science and Techniques, nos. 3: 314-18. https://doi.org/10.31797/vetbio.2054243.
EndNote
Nogay S, Dolanbay T, Gezer T, Demir MS, Demir B, Sezer M, Ayyıldız Akın M (October 1, 2026) Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index. Journal of Advances in VetBio Science and Techniques 3 314–318.
IEEE
[1]S. Nogay et al., “Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index”, J Ad VetBio Sci Tech, no. 3, pp. 314–318, Oct. 2026, doi: 10.31797/vetbio.2054243.
ISNAD
Nogay, Süleyman - Dolanbay, Turgut - Gezer, Tahir - Demir, Merve Sena - Demir, Bilgehan - Sezer, Mehmet - Ayyıldız Akın, Merve. “Evaluation of Cyclophosphamide-Induced Toxicity in Rats in Terms of Catalase, Nitric Oxide, Total Antioxidant and Total Oxidant Status, and Oxidative Stress Index”. Journal of Advances in VetBio Science and Techniques. 3 (October 1, 2026): 314-318. https://doi.org/10.31797/vetbio.2054243.
JAMA
1.Nogay S, Dolanbay T, Gezer T, Demir MS, Demir B, Sezer M, Ayyıldız Akın M. Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index. J Ad VetBio Sci Tech. 2026;:314–318.
MLA
Nogay, Süleyman, et al. “Evaluation of Cyclophosphamide-Induced Toxicity in Rats in Terms of Catalase, Nitric Oxide, Total Antioxidant and Total Oxidant Status, and Oxidative Stress Index”. Journal of Advances in VetBio Science and Techniques, no. 3, Oct. 2026, pp. 314-8, doi:10.31797/vetbio.2054243.
Vancouver
1.Süleyman Nogay, Turgut Dolanbay, Tahir Gezer, Merve Sena Demir, Bilgehan Demir, Mehmet Sezer, Merve Ayyıldız Akın. Evaluation of cyclophosphamide-induced toxicity in rats in terms of catalase, nitric oxide, total antioxidant and total oxidant status, and oxidative stress index. J Ad VetBio Sci Tech. 2026 Oct. 1;(3):314-8. doi:10.31797/vetbio.2054243

 

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