A comparison of Activating Solutions with Hatchery Water in Artificial Insemination of Rainbow Trout (Oncorhynchus mykiss)

Year 2018, , 8 - 15, 31.08.2018

Burak Evren İnanan , Ümit Acar , Hüseyin Urçuk Ersin Çelik

https://doi.org/10.31797/vetbio.396372

Abstract

In the propagation of rainbow
trout, the available water supply used for incubation of fertilized egg is
generally used for also spermatozoa being activated to reach eggs. The aim of
the present study was to assess comparisons of the effects of two lab-made
activating solutions and hatchery water on progressive sperm motility
percentage (%), duration of progressive sperm motility (s), and fertilization
success in artificial insemination of rainbow trout. For this purpose, an
activating solution (A1) containing 60 mM NaHCO₃, 50 mM Tris pH=9.0 and
another activating solution (A2) containing 20 mM Tris, 30 mM glycine, 125 mM
NaCl, pH=9.0, and also  hatchery water
(HW) were used for activation of spermatozoa and fertilization. The average
motility percentages of samples activated by HW, A1 and A2 were observed
>90% with no significant differences, while the durations of progressive
motility were found to be significantly different as 22.5±0.7 s, 30.0±1.4 s and
30.5±0.7 s respectively. The lowest average fertilization rate (64.6±1.4 %) was
obtained using HW, while those values were 89.4±5.1 and 91.3±0.6 % using A1 and
A2 respectively. Consequently, both motility durations and fertilization rates
obtained by using A1 and A2 were significantly higher than those values of
obtained by HW.

Keywords

Activating solutions, hatchery water, artificial insemination, rainbow trout

Gökkuşağı Alabalığı (Oncorhynchus mykiss) Suni Döllemesinde Aktivasyon Solüsyonları ve Kuluçka Suyunun Karşılaştırılması

Abstract

Gökkuşağı alabalığı üretimin,
genellikle döllenmiş yumurtaların inkübasyonu için kullanılan mevcut su kaynağı
aynı zamanda, suni döllemede sperm hücrelerinin aktivasyonu içinde kullanılır. Bu
çalışma laboratuvarda hazırlanmış aktivasyon solüsyonlarının ve kuluçka suyunun
doğrusal motilite yüzdesi (%), doğrusal motilite süresi (s) ve döllenme
oranlarına etkilerinin değerlendirilmesini amaçlamıştır. Bu amaçla, biri 60 mM
NaHCO₃, 50 mM Tris pH=9,0 içeriğine sahip (A1), diğeri 20 mM Tris,
30 mM glycine, 125 mM NaCl, pH=9.0 içeriğine sahip (A2) iki farklı aktivasyon
solüsyonu ve kuluçka suyu (HW) spermatozoa aktivasyonu ve suni dölleme de
kullanılmıştır. HW, A1 ve A2 ile aktive edilmiş örneklerin ortalama motilite
yüzdeleri, istatistiki fark olmaksızın > %90 olarak gözlenmişken, doğrusal
motilite süreleri, sırasıyla 22.5±0.7 s, 30.0±1.4 s ve 30.5±0.7 s olarak
kaydedilmiş ve önemli derecede fark olduğu bulunmuştur. En düşük ortalama
döllenme oranı (% 64.6±1.4) HW kullanılarak elde edilmişken, bu oranların A1 ve
A2 kullanıldığında %89.4±5.1 ve 91.3±0.6 olarak kaydedilmiştir. Sonuç olarak,
A1 ve A2 kullanılarak elde edilen hem motilite süreleri hem de döllenme
oranları, HW kullanılarak elde edilenlerden önemli derecede yüksek olduğu
belirlenmiştir. 

Keywords

Aktivasyon solüsyonları, kuluçka suyu, suni dölleme, gökkuşağı alabalığı

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