A comparison of Activating Solutions with Hatchery Water in Artificial Insemination of Rainbow Trout (Oncorhynchus mykiss)

Abstract

In the propagation of rainbow trout, the available water supply used for incubation of fertilized egg is generally used for also spermatozoa being activated to reach eggs. The aim of the present study was to assess comparisons of the effects of two lab-made activating solutions and hatchery water on progressive sperm motility percentage (%), duration of progressive sperm motility (s), and fertilization success in artificial insemination of rainbow trout. For this purpose, an activating solution (A1) containing 60 mM NaHCO₃, 50 mM Tris pH=9.0 and another activating solution (A2) containing 20 mM Tris, 30 mM glycine, 125 mM NaCl, pH=9.0, and also  hatchery water (HW) were used for activation of spermatozoa and fertilization. The average motility percentages of samples activated by HW, A1 and A2 were observed >90% with no significant differences, while the durations of progressive motility were found to be significantly different as 22.5±0.7 s, 30.0±1.4 s and 30.5±0.7 s respectively. The lowest average fertilization rate (64.6±1.4 %) was obtained using HW, while those values were 89.4±5.1 and 91.3±0.6 % using A1 and A2 respectively. Consequently, both motility durations and fertilization rates obtained by using A1 and A2 were significantly higher than those values of obtained by HW.

Keywords

• Activating solutions,hatchery water,artificial insemination,rainbow trout

Gökkuşağı Alabalığı (Oncorhynchus mykiss) Suni Döllemesinde Aktivasyon Solüsyonları ve Kuluçka Suyunun Karşılaştırılması

Abstract

Gökkuşağı alabalığı üretimin, genellikle döllenmiş yumurtaların inkübasyonu için kullanılan mevcut su kaynağı aynı zamanda, suni döllemede sperm hücrelerinin aktivasyonu içinde kullanılır. Bu çalışma laboratuvarda hazırlanmış aktivasyon solüsyonlarının ve kuluçka suyunun doğrusal motilite yüzdesi (%), doğrusal motilite süresi (s) ve döllenme oranlarına etkilerinin değerlendirilmesini amaçlamıştır. Bu amaçla, biri 60 mM NaHCO₃, 50 mM Tris pH=9,0 içeriğine sahip (A1), diğeri 20 mM Tris, 30 mM glycine, 125 mM NaCl, pH=9.0 içeriğine sahip (A2) iki farklı aktivasyon solüsyonu ve kuluçka suyu (HW) spermatozoa aktivasyonu ve suni dölleme de kullanılmıştır. HW, A1 ve A2 ile aktive edilmiş örneklerin ortalama motilite yüzdeleri, istatistiki fark olmaksızın > %90 olarak gözlenmişken, doğrusal motilite süreleri, sırasıyla 22.5±0.7 s, 30.0±1.4 s ve 30.5±0.7 s olarak kaydedilmiş ve önemli derecede fark olduğu bulunmuştur. En düşük ortalama döllenme oranı (% 64.6±1.4) HW kullanılarak elde edilmişken, bu oranların A1 ve A2 kullanıldığında %89.4±5.1 ve 91.3±0.6 olarak kaydedilmiştir. Sonuç olarak, A1 ve A2 kullanılarak elde edilen hem motilite süreleri hem de döllenme oranları, HW kullanılarak elde edilenlerden önemli derecede yüksek olduğu belirlenmiştir. 

Keywords

• Aktivasyon solüsyonları,kuluçka suyu,suni dölleme,gökkuşağı alabalığı

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