Investigation of respiratory syncytial virus (RSV) and parainfluenza-3 (PI-3) virus by histopathological and immunohistochemical methods in sheep and goat

Year 2022, Volume: 7 Issue: 1, 100 - 108, 30.04.2022

Funda Terzi , Ayşe Gül Dal , Serdar Odacı , Yavuz Ulusoy , Bahadır Kılınç

https://doi.org/10.31797/vetbio.976306

Abstract

Koyun ve keçilerde Respiratuar Sinsitiyal Virüsü (RSV) ve Parainfluenza tip 3 (PI3) virüsü ciddi solunum sistemi enfeksiyonlarına neden olmaktadır. Çalışmada, koyun ve keçi RSV ve PI-3 virüsünün akciğer dokusundaki yerleşimi ve dağılımının histopatolojik ve immünohistokimyasal yöntemlerle araştırılması amaçlandı. Çalışma materyalini, 2016-2019 yılları arasında, Samsun ve çevre yörelerinden Samsun Kontrol ve Veteriner Araştırma Enstitüsüne gelen 30 adet koyun ve 24 adet keçi akciğer parafin blok dokusu oluşturdu. Akciğer parafin blok dokularının histopatolojik incelemesinde, bronş ve bronşiyol epitelinde dejenerasyon ve dökülme, interalveolar septumda kalınlaşma, epitelizasyon ve alveolar makrofajlarda artış yaygın olarak görülürken, lenfoid doku da hiperplazi, hiyalin membran oluşumu ve sinsityal hücrelere daha az oranda rastlandı. Ayrıca hiçbir olguda bronşiolitis obliteransa rastlanmadı. İmmünhistokimyasal olarak, koyunların %50'sinde ve keçilerin %54'ünde RSV antijeni, koyunların %40'ında ve keçilerin %50'sinde PI-3 antijeni tespit edildi. Koyun ve keçi akciğer parafin kesitlerinde RSV ve PI-3 antijeni, bronş ve bronşiol epitel hücreleri ile lümende dökülmüş hücrelerinde ve interalveolar septum hücrelerinde istatistiksel olarak benzerdi (P>0.05). Alveolar makrofajlarda keçilerde PI-3 antijeni, koyunlara göre istatistiksel olarak daha yoğun boyandı(P<0.05).
Koyun ve keçi akciğer dokusunda RSV ve PI-3 antijeninin replikasyon alanları benzer olduğu kanısına varıldı. Koyun ve keçi akciğer dokusunda, RSV ve PI-3 virusun neden olduğu sitokin yanıt mekanizmasının moleküler yöntemlerle araştırılması gerektiği düşünülmektedir.

Keywords

RSV, PI-3, Immunohistochemistry, Sheep, Goat.

Supporting Institution

Kastamonu Scientific Research project (Project No: ).

Project Number

KÜ-BAP 01/2019-28

Thanks

We would like to thank Expert Veterinarian İsmail AYDIN of the Samsun Veterinary Control Institute Director.

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