Profile of Secondary Metabolites in Different Part of the Butterfly Pea (Clitoria ternatea) Plant with Antioxidant Activity

Yıl 2023, , 231 - 247, 30.06.2023

Waras Nurcholis , Tenku Muhammad Iqbal Sulistiyani Sulıstıyanı Novia Lıwanda

https://doi.org/10.29133/yyutbd.1251495

Cited By: 3

Öz

The butterfly pea flower (Clitoria ternatea) is widely used in traditional medicine and has the potential to be an antioxidant. The study aimed to compare the antioxidant capacity of the ethanol extract of the butterfly pea flower and the metabolite profile of the n-hexane fraction in different parts of the plant. The butterfly pea flower was planted for 12 weeks, and as many as 30 plants were in the Tropical Biopharmaca Research Center Cikabayan experimental garden, Bogor, Indonesia. Plant measurements included plant height, number of leaves, stem diameter, and plant production, which always increased during the experiment. The root had the highest phenolic content of each part of the plant (roots, flowers, leaves, and stems), with a phenolic content of 83.45 mg GAE/g. At the same time, the highest flavonoid content was in the leaves, with a total flavonoid value of 5.96 mg QE/g. Flowers and leaves only have anthocyanin content. The root had the highest antioxidant activity (low IC50 value) of each part of the plant, with an IC50 value of 106.973 µg/mL. The GC-MS results from the roots showed 13 compounds identified: 12 in the flower parts, 11 in the leaf parts, and 9 in the stem parts.

Anahtar Kelimeler

Antioxidant, Ethanol extract, Pea flower, GC-MS, Secondary metabolites

Kaynakça

• Adwas, A. A., Elsayed, A., Azab, A. E., & Quwaydir, F. A. (2019). Oxidative stress and antioxidant mechanisms in human body. J. Appl. Biotechnol. Bioeng, 6(1), 43-47. https://doi.org/10.15406/jabb.2019.06.00173
• Alam, M. A., Islam, P., Subhan, N., Rahman, M. M., Khan, F., Burrows, G. E., ... & Sarker, S. D. (2021). Potential health benefits of anthocyanins in oxidative stress related disorders. Phytochemistry reviews, 20(4), 705-749. https://doi.org/10.1007/s11101-021-09757-1
• Alp, Ş., Zarıfıkhosroshahı, M. , Yaşa Öztürk, G. & Ercisli, S. (2022). Extraction and Identification of Volatile Compounds in Rosa laxa Retz var harputense T. Baytop "Kişmiri rose". Yuzuncu Yıl University Journal of Agricultural Sciences , 32 (4) , 734-739 . DOI: 10.29133/yyutbd.1136189
• Artiyani, A., Roostrianawaty, N., Dwiratna, C., Andjar, S., & Ibrahim, M. (2023). Development of Telang Flower Management for PKK Empowerment at Perum Puri Cempaka Putih 1 RT 04 RW 05 Malang City Guan Economic Improvement. Asian Journal of Community Services, 2(1), 117-124. https://doi.org/10.55927/ajcs.v2i1.2863
• Borges, L. L., Alves, S. F., Sampaio, B. L., Conceição, E. C., Bara, M. T. F., & Paula, J. R. (2013). Environmental factors affecting the concentration of phenolic compounds in Myrcia tomentosa leaves. Revista Brasileira de Farmacognosia, 23(2), 230-238. https://doi.org/10.1590/S0102-695X2013005000019
• BPOM RI Drug and Food Control Agency of the Republic of Indonesia. 2008. Mutu keamanan dan kemanfaatan suatu produk bahan alam 2. Naturakos, 8(8), 1-3.
• Depkes RI 2008. Farmakope Herbal Indonesia (1st Ed.). Jakarta, ID: Ministry of Health of the Republic of Indonesia.
• El-Maati, M. F. A., Mahgoub, S. A., Labib, S. M., Al-Gaby, A. M., & Ramadan, M. F. (2016). Phenolic extracts of clove (Syzygium aromaticum) with novel antioxidant and antibacterial activities. European Journal of Integrative Medicine, 8(4), 494-504. https://doi.org/10.1016/j.eujim.2016.02.006
• Erviani, A. E., & Arif, A. R. (2017). Rendemen analysis and phytochemical screening of Perinereis aibuhitensis extract. International Journal of Current Research and Academic Review, 5(11), 25-29. https://doi.org/10.20546/ijcrar.2017.511.005
• Esmail, A. (2016). Pharmacological importance of Clitoria ternatea – A review. Journal of Pharmacy, 6(3), 68-83.
• Ferraz, R. C., Foss-Freitas, M. C., Vidal, T. R., Griffo, T. N., Concalves, N. B., Jordau, A. A., & Foss, M. C. 2018. Alpha-linolenic acid supplementation is associated with changes in inflammatory markers and endoplasmic reticulum stress in diabetic rats. Journal of Food Processing and Technology, 9(3), 1-3. https://doi.org/10.4172/2157-7110.1000720
• Fidrianny, I., Rizkiya, A., & Ruslan, K. (2015). Antioxidant activities of various fruit extracts from three Solanum sp. using DPPH and ABTS method and correlation with phenolic, flavonoid and carotenoid content. Journal of Chemical and Pharmaceutical Research, 7(5), 666-672.
• Gengaihi, S. E., Ella, F. M. A., Shalaby, E., & Doha, H. (2014). Antioxidant activity of phenolic compounds from different grape wastes. Journal Food Process Technology, 5(2), 1-5. http://dx.doi.org/10.4172/2157-7110.1000296
• Ghazali, Q., & Yasin, N. H. M. (2016). The effect of organic solvent, temperature and mixing time on the production of oil Moringa oleifera seeds. IOP Conference Series: Earth and Environmental Science, 36(1), 1-7. https://doi.org/10.1088/1755-1315/36/1/012053
• Kadam, V. B., & Ahire, P. P. (2011). Determination of extractive percentage of Clitoria ternatea. Life Science Bulletin, 8(1), 85-86.
• Kamiloglu, S., Capanoglu, E., Grootaert, C., & Camp, J. V. (2015). Anthocyanin absorption metabolism by human intestinal Caco-2 Cells-A review. International Journal of Molecular Sciences, 16, 21555-21574. https://www.mdpi.com/1422-0067/16/9/21555#
• Kaur, N., Chahal, K. K., Kumar, A., Singh, R., & Bhardwaj, U. (2019). Antioxidant activity of Anethum graveolens L. essential oil constituents and their chemical analogues. Journal of Food Biochemistry, 43(4), 12782. https://doi.org/10.1111/jfbc.12782
• Kumar S, Singh R, Gond DK. 2017. Diversity of rust fungus Puccinia on Justicia. Plant Pathology & Quarantine. 7(1): 53-58.
• Lee, P. M., Abdullah, R., & Hung, K. L. (2011). Thermal degradation of blue anthocyanin extract of Clitoria ternatea flower. International Proceeding of Chemical, Biological and Environmental Engineering, 7(1), 49-53.
• Lijon, M. B., Meghla, N. S., Jahedi, E., Rahman, M. A., & Hossain, I. (2017). Phytochemistry and pharmacological activities of Clitoria ternatea. International Journal of Natural and Social Sciences, 4(1), 1-10.
• Mohandas, G. G., & Kumaraswamy. (2018). Antioxidant activities of terpenoid from Thuidium tamariscellum (C.Muell.) Bosch. and Sande-Lac. a Moss. Pharmacognosy Journal, 10(4), 645-649. http://dx.doi.org/10.5530/pj.2018.4.106
• Mus, A. A., Goh, L. P. W., Marbawi, H., & Gansau, J. A. (2022). The Biosynthesis and Medicinal Properties of Taraxerol. Biomedicines, 10(4), 807. https://doi.org/10.3390/biomedicines10040807
• Neda, G. D., Rabeta, M. S., & Ong, M. T. (2013). Chemical composition and anti-proliferative properties of flowers of Clitoria ternatea. Internation Food Research Journal, 20(3), 1229-1234.
• Parvathi, M., & Ravishankar, K. (2013). Evaluation of antidepressant, motor coordination and locomotor activities of ethanolic root extract of Clitoria ternatea. Journal of Natural Remedies, 19-24. https://doi.org/10.18311/jnr/2013/113
• Rabeta, M. S., & Nabil, Z. (2013). Total phenolic compounds and scavenging activity in Clitoria ternatea and Vitex negundo linn. International Food Research Journal, 20(1), 495-500.
• Setford, P. C., Jeffery, D. W., Grbin, P. R., & Muhlack, R. A. (2017). Factors affecting extraction and evolution of phenolic compounds during red wine maceration and the role of process modelling. Trends in Food Science & Technology, 69, 106-117. https://doi.org/10.1016/j.tifs.2017.09.005
• Sholikhah, E. N. (2016). Indonesian medicinal plants as sources of secondary metabolites for pharmaceutical industry. Journal of Medical Sciences, 48(4), 226-239. http://dx.doi.org/10.19106/JMedSci004804201606
• Suarna, I. W., & Wijaya, I. M. S. (2021). Butterfly pea (Clitoria ternatea L.: Fabaceae) and its morphological variations in Bali. Journal of Tropical Biodiversity and Biotechnology, 6(2), 63013. https://doi.org/10.22146/jtbb.63013
• Šulc, M., Kotíková, Z., Paznocht, L., Pivec, V., Hamouz, K., & Lachman, J. (2017). Changes in anthocyanidin levels during the maturation of color-fleshed potato (Solanum tuberosum L.) tubers. Food Chemistry, 237, 981-988. https://doi.org/10.1016/j.foodchem.2017.05.155
• Surapaneni, S., & Prakash, T. (2018). Comprhenesive assignment of extraction, isolation and characterization of taraxerol from bark Annona reticula L. and chemopreventive effect on human prostate cancer cell lines (LNDaP and PC-3). Journal Carcinogenesis and Mutagenesis, 9(1), 1-3.
• Taslim, N. A., Yuliana, I., Djide, M. N., & Rifai, Y. (2021). Antioxidant activity of binahong (Anredera cordifolia (Tenore) Steen) simplicia Leaves. Nveo-Natural Volatiles & Essential Oils Journal| NVEO, 8(5), 4413-4419.
• Thirumurugan, D., Cholarajan, A., Raja, S. S., & Vijayakumar, R. (2018). An introductory chapter: secondary metabolites. Secondary Metabolites-sources and Applications, 3-21. http://dx.doi.org/10.5772/intechopen.79766
• Thuy, N. M., Ben, T. C., Minh, V. Q., & Van Tai, N. (2021). Effect of extraction techniques on anthocyanin from butterfly pea flowers (Clitoria ternatea L.) cultivated in Vietnam. Journal of Applied Biology and Biotechnology, 9(6), 173-180. http://dx.doi.org/10.7324/JABB.2021.96022
• Tonutare, T., Moor, U., & Szajdak, L. (2014). Strawberry anthocyanin determination by pH differential spectroscopic method–how to get true results? Acta Scientiarum Polonorum Hortorum Cultus, 13(3), 35-47.
• Treml, J., & Smejkal, K. (2016). Flavonoids as potent scavengers of hydroxyl radical. Comprehensive Reviews in Food Science and Food Safety, 15(4), 720- 738. https://doi.org/10.1111/1541-4337.12204
• Vats, S. (2014). Antioxidant activity of Clitoria ternatea L. and Origanum vulgare L.: a comparative analysis. Researcher, 6(11), 56-58.
• Wijit, N., Prasitwattanaseree, S., Mahatheeranont, S., Wolschann, P., Jiranusornkul, S., & Nimmanpipug, P. (2017). Estimation of retention time in GC/MS of volatile metabolites in fragrant rice using principle components of molecular descriptors. Analytical Sciences, 33(11), 1211-1217. https://doi.org/10.2116/analsci.33.1211
• Yara-Varon, E., Fabiano-Tixier, A. S., Balcells, M., Canela-Garayoa, R., Bily, A., & Chemat, F. (2016). Is it possible to substitute hexane with green solvents for extraction of carotenoids? A theoretical versus experimental solubility study. RSC Advances, 6(33), 27750-27759. https://doi.org/10.1039/C6RA03016E