Discover the Most Effective Disease Management Strategies for Fusarium Dry Rot of Potato through Comprehensive Bio-assay of Three Techniques (Chemical, Plant extracts, and Bio-control)

Abstract

Potato (Solanum tuberosum) is a multicultural staple food and cash crop. Unfortunately, production of potatoes is predominantly constrained by diseases including dry rots. To evaluate management of dry rots, three in vitro sub-trials were set up using the completely randomized design with each treatment replicated thrice for each trial. Firstly, synthetic fungicides were assayed against Fusarium oxysporum f.sp. tuberosi. Secondly, plant extracts were assessed against F. oxysporum. Finally, the efficacy of Trichoderma harzianum applied against F. oxysporum was evaluated. The colony radii were measured. The inhibition of F. oxysporum by Ketoconazole (at 100% concentration) was significantly (p≤0.05) highest, followed by Ketoconazole (50% concentration), Itraconazole (100% concentration), Itraconazole (50% concentration), Sulphur (100% concentration), Ridomil (100% concentration), Sulphur (50% concentration), and finally Ridomil (50% concentration). Percentage inhibition of the growth of the Fusarium species by fungicides ranged from 39.5-95.7%. Blue gum (Eucalyptus globulus) (at 100% concentration) gave the highest inhibition, followed by blue gum (50% concentration), Sweet alligator-pepper (Aframomum melegueta) at 100% concentration, locust bean (Parkia biglobosa) at 100% concentration, Sweet alligator-pepper (50% concentration), candle bush (Senna alata) (100% concentration), locust bean (50% concentration), and Senna alata (50% concentration) in descending order of percentage inhibition. Plant extracts caused a percentage inhibition of the fungus between 20.6-100% inhibition with time. Inhibition of Fusarium by T. Harzianum isolate BGMZ4 was significantly (p≤0.05) highest, followed by T. Harzianum isolate NSBM then T. Harzianum isolate BGMZ3. Control of F. Oxysporum by T. Harzianum ranged from 23.5-94.1% inhibition. All the methods evaluated successfully inhibited the pathogen compared to the control.

Keywords

• fungicide
• plant extracts
• Trichoderma species
• post-harvest disease
• Solanum tuberosum

Supporting Institution

no

Ethical Statement

Ethics committee This is not applicable to this research. Thanks Ndifon EM, Ph.D

Thanks

thanks Sir

References

1. Adolf, B., Andrade-Piedra, J., Bittara, M. F., Przetakiewicz, J., Hausladen, H., & Kromann, P. (2020). Fungal, oomycete, and plasmodiophorid diseases of potato. Cham: Springer. https://doi.org/10.1007/978-3-030-28683-5
2. Amienyo, C., & Onuze, E. (2015). In vitro evaluation of Carica papaya L. and Lantana camara L. extracts in the control of late blight of potato caused by Phytophthora infestans (Mont.) de Bary. Agricultural Science Research Journal, 5, 187–194.
3. Abdu, H. I., Abubakar, I., Dingari, M., Ibrahim, U. K., & Waja, S. (2020). Fungicidal effect of three plant extracts in control of four phytopathogenic fungi of Irish potato (Solanum Tuberosum.) fruit rot in Mubi Adamawa State. African Scholar Publications and Research International, 18(2). ISSN: 2010-1086.
4. Ab Rahman, A., Hamid, U. Z. A., & Chin, T. A. (2017). Emerging technologies with disruptive effects: A review. Perintis Ejournal, 7, 111–112
5. Ayed, F., Daami-Rema, M., Jabnoun-Kh, H., & Mahjoub, M. E. (2007). In vitro and In vivo evaluation of some biofungicides for potato Fusarium wilt biocontrol. International Journal of Agricultural Research, 2(3), 282–288.
6. Al-Mughrabi, K. I., Vikram, A., Poirier, R., Jayasuriya, K., & Moreau, G. (2016). Management of common scab of potato in the field using biopesticides, fungicides, soil additives, or soil fumigants. Biocontrol Science Technololgy, 26, 125–135. Doi: 10.1080/09583157.2015.1079809
7. Asaturova, A., Shternshis, M., Tsvetkova, V., Shpatova, T., Maslennikova, V., Zhevnova, N., & Homyak, A. (2021). Biological control of important fungal diseases of potato and raspberry by two Bacillus velezensis strains. Peer Journal, 9, e11578. Doi: 10.7717/peerj.11578.
8. Aydın, M. H., & İnal, B. (2018). Comparative susceptibility of some commercial potato cultivars to Fusarium sambucinum and F.solani isolates causing tuber dry rot. Applied Ecology and Environmental Research, 16(4), 4879-4892. http://dx.doi.org/10.15666/aeer/1604_48794892

9. Aydın, M. H. (2019). Evaluation of some Trichoderma species in biological control of potato dry rot caused by Fusarium sambucinum Fuckel isolates. Applied Ecology and Environmental Research, 17 (1), 533-546. http://dx.doi.org/10.15666/aeer/1701_533546
10. Barnett, H. L., & Hunter, B. B. (1972). Illustrated genera of imperfect fungi. 3rd Edition, Burgess Publishing Co., Minneapolis. 241 pages.
11. Bojanowski, A., Avis, T. J., Pelletier, S., & Tweddell, R. J. (2013). Management of potato dry rot. Postharvest Biology and Technology, 84, 99-109. DOI:10.1016/j.postharvbio.2013.04.008
12. Chatterjee, D. (1990). Inhibition of fungal growth and infection in maize grains by spice oils. Letter on Applied Microbilogy, 11, 148-151
13. Chatri, M., Advinda, L., Oktıana, F., Malona, P., Lathifah, S., & Putri, D. H. (2024). Exploration and investigation of antifungal activity of plant leaf extracts on growth of Scletorium rolfsii sacc. Yuzuncu Yıl University Journal of Agricultural Sciences, 34(2), 199-210. https://doi.org/10.29133/yyutbd.1381441
14. Chen, D., Nahar, K., Bizimungu, B., Soucy, S., Peters, R. D., De Koeyer, D., & Dickison, V. (2020). A Simple and efficient inoculation method for fusarium dry rot evaluations in potatoes. American Journal of Potato Research, 97, 265–271.
15. Cullen, D. W., Toth, I. K., Pitkin, Y., Boonham, N., Walsh, K., Barker, I., & Lees, A. K. (2005). Use of quantitative molecular diagnostic assays to investigate Fusarium dry rot in potato stocks and soil. Phytopathology, 95, 1462–1471.
16. Devaux, A., Goffart, J. P., Petsakos, A., Kromann, P., Gatto, M., Okello, J., Suarez, V., & Hareau, G. (2019). Global food security, contributions from sustainable potato agri-food systems. In: The potato crop: its agricultural, nutritional and social contribution to humankind. Springer International Publishing: Cham, Switzerland. pp. 3–35. ISBN 9783030286835.
17. Draper, M. A., Secor, G. A., & Lamey, H. A. (1994). Management of potato diseases in the home garden. PP.756 (Revised). www.ag.ndsu.edu.
18. Du, M., Ren, X., Sun, Q., Wang, Y., & Zhang, R. (2012). Characterization of Fusarium spp. causing potato dry rot in China and susceptibility evaluation of Chinese potato germplasm to the pathogen. Potato Research, 55, 175–184.
19. Ehiobu, J., Idamokoro, E., & Afolayan, A. (2022). Biofungicides for improvement of potato (Solanum tuberosum L) production. A review. Article ID: 1405900. https://doi.org/10.1155/2022/1405900
20. Gopal, J., & Khurana, P. S. M. (2006). Handbook of potato production, improvement, and postharvest. Haworth. http://books.google.com/?id=hxy8pkP26NEC&pg.
21. Gupta, C. P., Sharma, A., Dubey, R. C., & Maheshwari, D. K. (1999). Pseudomonas aeruginosa (GRCI) as a strong antagonist of Macrophomina phaseolina and Fusarium oxysporum. Cytobios, 99, 183-189.
22. Horton, D. (1992). La papa: Produccion, commercialization y programas. In: Potato viruses and their control L. F. Salazar, (ed). CIP, Lima, Peru. 214 pages.
23. Kankoranta T. (1996). Impact of globe warming on potato late blight: risk, yield loss and control. Agricultural and Food Science in Finland, 5, 311-327.
24. Khare, K. B., Loeto, D., Wale, K., & Salani, M. (2016). Seed-borne fungi of cowpea (Vigna unguiculata (L.) Walp) and their possible control in vitro using locally available fungicides in Botswana. International Journal of Bioassays, 5(11), 5021-5024.
25. Kim, S., Hong, S. J, Won, H. S., Kim, B. S., & Gwon, S. H. (2024). Identification and pathogenicity of species isolated from stored potato tubers showing symptoms of dry rot disease. Potato Research. 1-12. https://link.springer.com/article/10.1007/s11540-024-09709-0
26. Larkin, R. P., & Lynch, R. P. (2018). Use and effects of different brassica and other rotation crops on soilborne diseases and yield of potato. Horticulturae, 4, 37.
27. Leach, S. S., & Nelson, L. W. (1975). Elimination of fusarial contamination on seed potatoes. American Potato Journal, 9, 211-218.
28. Lee, T. R., Anderson, S. J., Tran-Nguyen, L. T., Sallam, N., Le Ru, B. P., & Conlong, D. (2019). Towards a global DNA barcode reference library for quarantine identifications of lepidopteran stemborers, with an emphasis on sugarcane pests. Science Report, 9, 1–19. Doi: 10.1038/s41598-019-42995-0
29. Liu, J., Sun, Z., Zou, Y., Li, W., He, F., & Huang, X. (2020). Pre- and postharvest measures used to control decay and mycotoxigenic fungi in potato (Solanum tuberosum L.) during storage. Critical Review of Food Science and Nutrition. pages 1–14. Doi: 10.1080/10408398.2020.1818688
30. Lugtenberg, B. (2018). Putting concerns for caution into perspective: microbial plant protection products are safe to use in agricultura. Journal of Plant Diseases and Protection, 125(2), 127–129. Doi: 10.1007/s41348-018-0149-5.
31. Masum, M. M. I., Islam, S. M. M., Islam, M. S., & Kabir, M. H. (2011). Estimation of loss due to post harvest diseases of potato in markets of different districts in Bangladesh. African Journal of Biotechnology, 10, 11892–11902.
32. Md. Mahi, I .M., & Nayem, H. (2023). Efficacy of Trichoderma harzianum, as a biological fungicide against fungal diseases of potato, late blight and early blight. Journal of Natural Pesticide Research, 5. 100047. www.journals.elsevier.com/journal-of-natural-pesticide-research.
33. Mengui, K. C., Oh, S., & Lee, S. H. (2019). The technical efficiency of smallholder Irish potato producers in Santa Subdivision, Cameroon. Agriculture, 9(12), 259. https://doi.org/10.3390/agriculture9120259
34. Merlington, A., Hanson, L. E., Bayma, R., Hildebrandt, K., Steere, L., & Kirk, W. W. (2014). First report of Fusarium proliferatum causing dry rot in Michigan commercial potato (Solanum tuberosum) production. Plant Disease, 98, 843.
35. Ministry of Agriculture. (2006). Strategic Plan 2006–2010; Mekong River Commission: Vientiane, Laos.
36. Mulema, J., Karanja, L., Otieno, W., Karanja, D., Macharia, I., Obare, I., Chepng’eno, M., Chemutai, C., Mugambi, I., Nyaundi, O., Wanjiku, J., Kagondu, M., Munguti, F., Ngundo, G., & Ochilo, W. (2021). Potato diseases surveillance in Kenya, final project report. CAB International (CABI) and Kenya Plant Health Inspectorate Services (KEPHIS), Nairobi, Kenya. 156 Pages.
37. Muthoni, J., Shimelis, H., & Crop, J.M-A. J. (2022). Production and availability of good quality seed potatoes in the East African region: A review. Australian Journal of Crop Science, 16, 907–915.
38. Ndifon, E. M. (2024). Compatibility of integrated management (chemical x biological, and botanical x biological) strategies deployed against Athelia rolfsii, causing Sclerotium rots. Bulgarian Journal of Crop Science, 61(3) 109-115. https://doi.org/10.61308/MMCO3405
39. Ndifon, E. M. (2023). Antimycotic inhibition of Phytophthora colocasiae (taro leaf blight) using diverse disease management paradigms. Indonesian Journal of Agricultural Research, 6(2), 126-136. DOI:10.32734/injar.v6i2.11913.
40. Ndifon, E. M., & Inyang, P. (2022). In vitro efficacy of disparate fungicides against Lasiodiplodia theobromae root rots of orange-fleshed sweet potato varieties. Indonesian Journal of Agricultural Science, 23(2), 56–64. DOI: http//dx.doi.org/10.21082/ijas.v.23.n2.2022.p.56–64
41. Ogunsola, J. F., & Aduramigba-Modupe, A. O. (2014). Evaluation of four plant extracts in the control of postharvest fungi tuber rot of Irish potato (Solanum tuberosum). African Journal of Root and Tuber Crops, 11(1), 1-9.
42. Orina, A. S., Gavrilova, O. P., Trubin, I. I., & Gagkaeva, T. Y. (2024). Effect of fungicides on Fusarium fungi caused potato dry rot. Agrohimiâ, 3:37-42. DOİ: 10.31857/S0002188124030057
43. Özakın, S., Taşkın, B., & İnce Bostancı, E. (2021). Identification and antimicrobial activity of actinobacteria isolated from rhizosphere of the local endemic plants. Yuzuncu Yıl University Journal of Agricultural Sciences, 31(4), 876-885. https://doi.org/10.29133/yyutbd.957756
44. Özcan, A. G. (2024). In vitro antifungal activity of Mentha piperita and Thymus vulgaris essential oils against ochratoxigenic Aspergillus carbonarius isolated from Bozcaada Çavuş Grape. Yuzuncu Yıl University Journal of Agricultural Sciences 34(1), 166-175. https://doi.org/10.29133/yyutbd.1403366
45. Riaz, M., Mahmood, R., Antar, M., Akhtar, N., Khan, S. N., & Anjum, M. A. (2022). A bacterial consortium and synthetic fertilizer based biocontrol approach against potato rot disease Neocosmospora rubicola. Lausanne: Frontiers Media SA.
46. Sagar, V., Sharma, S., Jeevalatha, A., Chakrabarti, S. K., & Singh, B. P. (2011). First report of Fusarium Sambucinum causing dry rot of potato in India. New Disease Report, 24, 5.
47. Shahnaz, D., Maimona, K., & Summiaya, R. (2015). Seed borne fungi associated with cowpea (Vigna unguiculata (l.) Walp. International Journal of Biology and Biotechnology, 12(4), 565-569.
48. Singh, A., & Singh, J. (2018). Integrated management of fungal diseases in potato. International Journal of Current Microbiology and Applied Sciences, 7(8), https://doi.org/10.20546/ijcmas.2018.708.469.
49. Tiwari, R. K., Kumar, R., Sharma, S., Naga, K.C., Subhash, S., & Sagar, V. (2020). Continuous and emerging challenges of silver scurf disease in potato. International Journal of Pest Management, 68, 89–101.
50. Tiwari, R. K., Lal, M. K., Kumar, R., Sharma, S., Sagar, V., Kumar, A., Singh, B., & Aggarwal, R. (2023). Impact of Fusarium infection on potato quality, starch digestibility, in vitro glycemic response, and resistant starch content. Journal of Fungi, 9(4), 466. https://doi.org/10.3390/jof9040466
51. Waterworth, K. (2023). Blight control in potatoes: how to treat early and late potato blight. Gardening Know How, Future US LLC. https://potato%20diseases%202023/potato-blight-diseases.htmL.
52. Xue, H., & Yang, Z. (2021). Potato dry rot caused by Fusarium spp. and mycotoxins accumulation and management. https://www.intechopen.com/chapters/79480. DOI: 10.5772/intechopen.100651
53. Xue, H., Liu, Q., & Yang, Z. (2023). Pathogenicity, mycotoxin production, and control of potato dry rot caused by Fusarium spp.: A Review. Journal of Fungi, 9(8), 843. https://doi.org/10.3390/jof9080843
54. Zongur, A. (2024). Efficacy of Beta vulgaris essential oils against potato dry rot disease and deoxynivalenol (DON) mycotoxin. SpringerLink Potato Research https://link.springer.com/article/10.1007/s11540-024-09742-z