Microalga Chlorella sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation

Marcelita Jeva; J-nadine Jalilul; Jurmin Sarri; Melodina Hairol

doi:10.29133/yyutbd.1666510

Microalga Chlorella sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation

Abstract

Microalgae, such as those from the genus Chlorella, produce biochemical compositions such as lipids, protein, and pigment. This research investigated the effects of different salinity levels in a nutrient medium on the growth and pigment synthesis of Chlorella sorokiniana. Microalga C. sorokiniana was cultured and grown in 500 mL glass bottle with varying concentrations of sodium chloride (10,15, 20, and 25 g L-1 NaCl) in a BG-11 medium, starting at an initial cell density of 2.68 x 105 cell mL-1. The cultures were maintained at 20 ± 1 °C, under continuous aeration, with a light intensity of 200 µmol photons m⁻² s⁻¹, a 24 h light photoperiod, and pH 7.5 ± 0.2. The results revealed that the optimal salinity concentration for enhancing the cell density, and the specific growth was 10 g L-1, demonstrating the highest cell density, exceeding the control group by 1.27-fold cell mL-1 at day 15 of the culture period. Additionally, the specific growth rate (SGR) was significantly higher in the 10 g L-1 of salinity concentration, achieving (0.05 ±0.14 day-1) as early as day 6 of the culture period compared to the other experimental groups. Cell size also increased significantly with 20 g L-1 of salinity concentration (49.91 ± 2.39 µm). Regarding the pigment accumulation, total carotenoid levels and chlorophyll-a, the elevated salinity concentration of 20 g L-1 suppresses chlorophyll-a accumulation and exhibited a reduction in total carotenoid pigment accumulation. Thus, these findings suggest that lower salinity levels (10 g L⁻¹ NaCl) can effectively enhance the growth of Chlorella sorokiniana, while higher salinity levels (20 g L⁻¹ NaCl) tend to suppress pigment production, particularly chlorophyll-a and total carotenoids accumulation.

Keywords

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Details

Primary Language

English

Subjects

Aquaculture and Fisheries (Other)

Journal Section

Research Article

Authors

Marcelita Jeva This is me
0009-0002-4314-0591
Philippines

J-nadine Jalilul
0009-0008-7388-1511
Philippines

Jurmin Sarri ^*
0000-0002-4798-0566
Philippines

Melodina Hairol This is me
0000-0002-2800-667X
Philippines

Publication Date

December 25, 2025

Submission Date

March 27, 2025

Acceptance Date

September 10, 2025

Published in Issue

Year 2025 Volume: 35 Number: 4

DOI

https://doi.org/10.29133/yyutbd.1666510

IZ

https://izlik.org/JA97PH79GT

Cite

RIS / Bibtex

APA

Jeva, M., Jalilul, J.- nadine, Sarri, J., & Hairol, M. (2025). Microalga Chlorella sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation. Yuzuncu Yıl University Journal of Agricultural Sciences, 35(4), 587-602. https://doi.org/10.29133/yyutbd.1666510

AMA

1.Jeva M, Jalilul J nadine, Sarri J, Hairol M. Microalga Chlorella sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation. YYU J AGR SCI. 2025;35(4):587-602. doi:10.29133/yyutbd.1666510

Chicago

Jeva, Marcelita, J-nadine Jalilul, Jurmin Sarri, and Melodina Hairol. 2025. “Microalga Chlorella Sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation”. Yuzuncu Yıl University Journal of Agricultural Sciences 35 (4): 587-602. https://doi.org/10.29133/yyutbd.1666510.

EndNote

Jeva M, Jalilul J- nadine, Sarri J, Hairol M (December 1, 2025) Microalga Chlorella sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation. Yuzuncu Yıl University Journal of Agricultural Sciences 35 4 587–602.

IEEE

[1]M. Jeva, J.- nadine Jalilul, J. Sarri, and M. Hairol, “Microalga Chlorella sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation”, YYU J AGR SCI, vol. 35, no. 4, pp. 587–602, Dec. 2025, doi: 10.29133/yyutbd.1666510.

ISNAD

Jeva, Marcelita - Jalilul, J-nadine - Sarri, Jurmin - Hairol, Melodina. “Microalga Chlorella Sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation”. Yuzuncu Yıl University Journal of Agricultural Sciences 35/4 (December 1, 2025): 587-602. https://doi.org/10.29133/yyutbd.1666510.

JAMA

1.Jeva M, Jalilul J- nadine, Sarri J, Hairol M. Microalga Chlorella sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation. YYU J AGR SCI. 2025;35:587–602.

MLA

Jeva, Marcelita, et al. “Microalga Chlorella Sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation”. Yuzuncu Yıl University Journal of Agricultural Sciences, vol. 35, no. 4, Dec. 2025, pp. 587-02, doi:10.29133/yyutbd.1666510.

Vancouver

1.Marcelita Jeva, J-nadine Jalilul, Jurmin Sarri, Melodina Hairol. Microalga Chlorella sorokiniana Response to Salinity: Effects on Cell Density, Size, and Pigment Accumulation. YYU J AGR SCI. 2025 Dec. 1;35(4):587-602. doi:10.29133/yyutbd.1666510