Hakkari İli Bal Arısı (Apis Mellifera L.) Kolonilerindeki Acute Bee Paralysis Virus (ABPV)’ünün Varlığının Saptanması ve Moleküler Karakterizasyonu

Öz

Hakkari ilinde 2014 yılı Mayıs ve Ağustos ayları arasında 90 farklı arılıkta yürütülen sürvey çalışmalarında Acute bee paralysis virus (ABPV)’ünün varlığı ve yaygınlığı araştırılmıştır. Viral nükleik asidi hassas ve güvenilir biçimde teşhis edebilmek için ABPV’nin genomunda yer alan kılıf protein genine spesifik olarak dizayn edilen ve yaklaşık 476 bç uzunluğunda DNA fragmenti üreten bir çift primer RT-PCR testlerinde kullanılmıştır. Sürvey gerçekleştirilen toplam 90 arılığın sadece iki tanesinde APBV infeksiyonu tespit edilmiş ve virüsün Hakkari ili bal arısı kolonilerinde yaygın olmadığı belirlenmiştir. Tespit edilen ABPV’nin dünyada bilinen diğer izolat ve ırklar ile olan genetik akrabalığını araştırmak için viral genomunda çoğaltılanfragmentler moleküler klonlama yöntemi ile klonlanmış ve DNA dizilemesi gerçekleştirilmiştir. Dizilemesi gerçekleştirilen izolat dünyanın farklı coğrafik bölgelerinde tespit edilen APBV’lere ait diziler ile karşılaştırılmıştır. YürütülenFilogenetik analizler sonucunda Hakkari ilinde tespit edilen APBV izolatının nükleotid dizisinin, Genbankasına kayıtlı dünyadaki diğer izolatlar ile % 85 ile 97 arasında benzerlik gösterdiği tespit edilmiştir. APBV’nin kısmi kılıf protein geni dizisi Gen Bankasına KP259311 ulaşım numarası ile kaydedilmiştir. 

Occurrence and Molecular Characterization of Acute Bee Paralysis Virus (ABPV) in Honeybee (Apis mellifera) Colonies in Hakkari Province

Abstract

A study was carried out during May-August 2014 to investigate the occurrence and prevalence of Acute bee paralysis virus (ABPV) in 90 different apiaries located in Hakkari province (Turkey). To detect the viral nucleic acids, an RT-PCR assay has been implemented for a sensitive and specific diagnosis of ABPV, by using a pair of primer specifically designed to coat protein gene of ABPV, amplifying a 476 bp nucleotide fragment. ABPV was found to occur in 2 samples from two distinct locations among 90 apiaries indicating that ABPV is not widespread in Hakkari province. To understand the molecular relationship between the isolates and the strains of the same virus, the amplified ABPV fragments cloned and sequenced and the sequence was compared with the fragments of ABPV sequences from different geographic origin of the world. The phylogenetic analysis revealed nucleotide identity rates between 85 and 97 %. The partial CP gene of ABPV sequence deposited in the GenBank database with the accession number of KP259311.

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