Çevresel Örneklerde Bulunan Canlı Patojen Mikroorganizmaları Saptama ve Miktarlarını Belirlemede Yeni Yaklaşımlar

Abstract

Gıda endüstrisi, çevre endüstrisi, tıp ve tarım gibi pek çok endüstri ve bilimsel alanda ürün kontaminasyonu halk sağlığı açısından çok kötü sonuçlar doğurabilmektedir. Bu nedenle çevresel ve endüstriyel örneklerde indikatör mikroorganizmaların saptanması ve miktarlarının belirlenmesi halk sağlığı ve güvencesi açısından çok büyük önem taşımaktadır. Patojen mikroorganizmaların canlı olanları büyük ölçüde halk sağlığını tehdit ettiği için sadece canlı hücreleri saptayan ve miktarlarını belirleyen yeni tekniklerin geliştirilmesi çok önemlidir. Bu derleme, bir örnek içerisinde bulunan sadece patojen mikroorganizmaları değil aynı zamanda istenilen diğer canlı hücreleri saptamakta ve miktarlarını belirlemekte kullanılan geleneksel yöntemler ile yeni, güncel moleküler gelişmeleri avantaj ve dezavantajları ile birlikte özetlemektedir.

Keywords

• Propidyum monoazid,Q-PCR,Canlı/Ölü hücre ayrımı,Patojen,Q-PCR

Novel Approaches for Monitoring Viable Pathogenic Microorganisms in Environmental Samples

Abstract

In many industries and scientific fields, for example, food industry, medicine, ecological business and agriculture, contamination of products may have terrible consequences in the manner of public health. Therefore, detection and quantification of indicator microorganisms in many environmental sample and industrial products are the keys for counteractive action and identification of problems related to health issues. Also, since viable portion of the pathogenic microorganisms is likely a threat to public health, developing a novel method to quantify only viable cells is imperative. This review considers the traditional methods and the novel molecular developments with their advantages and drawbacks for detection and quantification of not only pathogenic microorganisms but also any living cells in a sample.

Keywords

• Propidium monoazide,Q-PCR,Live/Dead differentiation,Pathogen,Q-PCR

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