Effect of Glyphosate on Some Protective Systems in Zea mays L.

Abstract

Glyphosate is an herbicide that is applied after non-selective germination and affects plant growth. In this study, glyphosate was applied to Zea mays L. after germination, at a concentration range from 0.017 to 0.145 M in a growth chamber. The effects of this herbicide on some antioxidant enzymes, lipid peroxidation, total chlorophyll and total carbohydrate content were investigated on days 1, 5 and 10 following the application. Results showed that peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) were increased in the groups treated with glyphosate on days 5 and 10, but the activities of reduced glutathione (GSH) and glutathione reductase (GR) were decreased on day 10. Malondialdehyde (MDA) content indicating lipid peroxidation was increased on days 1, 5 and 10. Changes in total chlorophyll and total carbohydrate contents were found to be time-dependent. These increases and decreases in the antioxidant activities, MDA content, total chlorophyll and total carbohydrate content in Zea mays L. were determined as a symptom of cytotoxicity caused due to glyphosate. 

Keywords

• Antioxidant,Glyphosate,Lipid peroxidation,Total chlorophyll,Total carbohydrate

Glifosatın Mısır (Zea mays L.)’da Bazı Koruyucu Sistemlere Etkisi

Öz

Glifosat bitki gelişimine etki eden seçici olmayan çimlenme sonrası ugulanan bir herbisittir. Bu çalışmada, glifosat Zea mays L.’a çimlenme sonrası 0.017-0.145 M konsantrasyon aralığında iklim odasında uygulanmıştır. Bu herbisitin bazı antioksidan enzimler, lipid peroksidasyonu, total klorofil ve toplam karbohidrat içeriği üzerindeki etkileri uygulamayı takiben 1., 5. ve 10. günlerde araştırılmıştır. Peroksidaz (POD), askorbat peroksidaz (APX), süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon-S-transferaz aktiviteleri glifosat uygulanan gruplarda 1. 5. ve 10. günlerde artış gösterirken, redükte glutatyon (GSH) ve glutatyon redüktaz (GR) aktivitesi 10. günde azalış göstermiştir. Lipid peroksidasyonunu gösteren malondialdehid (MDA) içeriği 1., 5. ve 10. günlerde artış göstermiştir. Total klorofil içeriği ve total karbohidrat içeriği zamana bağlı olarak değişim göstermiştir. Mısır’da antioksidan aktivite, MDA, toplam klorofil ve karbohidrat içeriğinde artış ve azalışın olması glifosattan dolayı bir sitotoksisite semptomu olarak saptanmıştır. 

Anahtar Kelimeler

• Antioksidan,Glifosat,Lipid peroksidasyonu,Toplam klorofil,Toplam karbohidrat

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