Germination and Emergence of Wild Rhubarb (Rheum ribes L.) Seeds In Vitro Culture Conditions

Abstract

Rheum ribes L. is a perennial wild plant that is of medical importance as flowers shoots and stalk can be consumed as vegetables. Due to their uncontrolled collection from their natural habitats, it is important to ensure the preservation and sustainability of these species that may be in danger of future extinction. In tissue culture techniques, in vitro micro-propagation studies with seed, in vitro optimum germination mechanism of the species should be known. This study was conducted to determine the in vitro germination and emergence of rhubarb seeds groups (seed tips were cut off or not). These groups were germinated in nutrient mediacombinations, including Murashige and Skoog (MS), Gamborg (B5), White (WH) and Schenk and Hildebrandt (SH) media with or without 200 mg/l gibberellic acid (GA₃). As a result of the research, it was determined that the seeds of this species have less severe physiological dormancy and the seed-tip cut applications are more successful in terms of germination and emergence parameters. The highest germination rate (84%) was obtained from MS+200 mg l^-1 GA₃ nutrient medium, and the best emergence rate (44%) was obtained from WH+200 mg l^-1 GA₃ medium.

Keywords

• Tissue culture,Rheum ribes L.,Seed

İn Vitro Kültür Koşullarında Yabani Ravent (Rheum ribes L.) Tohumlarında Çimlenme ve Çıkış

Öz

Yabani Ravent (Rheum ribes L.), çiçek sürgünleri ve sapları sebze olarak tüketilebilen tıbbi öneme sahip çok yıllık yabani bir bitkidir. Doğal habitatlarından kontrolsüz toplanmaları durumunda, ileride yok olma tehlikesiyle karşı karşıya kalabilecek bu türlerin korunması ve sürdürülebilirliğinin sağlanması önem taşımaktadır. Doku kültürü teknikleriyle tohumla in vitro mikro çoğaltım çalışmalarının yapılabilmesi için, türün in vitro koşullarda optimum çimlenme mekanizmasının bilinmesi gerekmektedir. Bu çalışma, uçları kesilmiş ve kesilmemiş yabani ravent tohum gruplarının in vitro koşullarda çimlenme ve çıkış durumlarının belirlenmesi amacıyla yapılmıştır. Bu gruplar, 200 mg/l giberellik asit (GA₃) ilavesi yapılmış veya yapılmamış, Murashige ve Skoog (MS), Gamborg (B5), White (WH) ve Schenk ve Hildebrandt (SH) ortam kombinasyonlarında in vitro olarak çimlendirilmiştir. Araştırma sonucunda, bu türün tohumlarında az şiddetli fizyolojik dormansinin görüldüğü ve tohum ucu kesilmemiş uygulamaların çimlenme ve çıkış parametreleri yönünden daha başarılı olduğu tespit edilmiştir. En yüksek çimlenme oranı (%84), MS+200 mg/l GA₃ besin ortamından, en iyi çıkış oranı ise (%44), WH+200 mg/l GA₃ ortamından sağlanmıştır.

Anahtar Kelimeler

• Rheum ribes L.,Tohum,Doku kültürü

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