Investigation of Total Flavonoid, DPPH Radical Scavenging, Lipid Peroxidation and Antimicrobial Activity of Allium schoenoprasum L. Plant Growing in Van Region

Yıl 2020, , 147 - 155, 31.03.2020

Yılmaz Koçak , Gökhan Oto , İsmet Meydan , Hamdullah Seçkin

https://doi.org/10.29133/yyutbd.674507

Cited By: 7

Öz

This study, the effect of ethanol extract of Allium schoenoprasum plant was investigated on some antioxidant parameters and pathogenic microorganisms that affect human health. In this coverage, DPPH (1,1-diphenyl-2-picrilhydrazyl) method was used to determine the antioxidant effect of A. schoenoprasum ethanol extract. Lipid peroxidation prevention activity was evaluated by TBA (Thiobarbituric acid) method. Besides, the antimicrobial activity of plant extract on some pathogenic bacteria was investigated via disk diffusion method. Quercetin was used to determine total flavonoid components. According to our findings, the total flavonoid component amount found in 0.2 mg/ml ethanol extract was found equivalent to quercetin with 44.465 ± 0.204 μg/ml. In addition, A. schoenoprasum showed that it was effective compared to BHA (Beta hydroxy acid), which is the positive control of lipid peroxidation activity. While the lowest and highest concentration values of DPPH radical Scavenging activity were 60,125 - 96,285%, these values were found to be 82.178 – 97.357% for BHA. Ethanol extract of A. schoenoprasum, was determined to show antimicrobial activity against some pathogenic microorganisms such as Bacillus subtilis, Escherichiacoli, Pseudomonas aeruginosa, Enterococcus faecalis, Candida albicans.

Anahtar Kelimeler

Antimicrobial, A. schoenoprasum L., DPPH, Flavonoid, Lipid Peroxidation

Van Bölgesinde Yetişen Allium schoenoprasum L. Bitkisinin Toplam Flavonoid, DPPH Radikal Söndürme, Lipid Peroksidasyonu ve Antimikrobiyal Aktivitesinin Araştırılması

Öz

Bu çalışma, Allium schoenoprasum bitkisinin etanol ektresinin, insan sağlığını etkileyen bazı antioksidan parametreler ve patojen mikroorganizmalar üzerindeki etkisi araştırılmıştır. Bu kapsamda, A. schoenoprasum etanol ekstresinin antioksidan etkisini belirlemek için DPPH (1,1-difenil-2-pikrilhidrazil) yöntemi kullanılmıştır. Lipid peroksidasyonunu önleme aktivitesi, TBA (Tiyobarbitürik asit) yöntemi ile değerlendirilmiştir. Ayrıca bitki ekstresinin bazı patojenik bakteriler üzerindeki antimikrobiyal etkinliği disk difüzyon yöntemi ile araştırılmıştır. Toplam flavonoid bileşenleri belirlemek içinde kuersetin kullanılmıştır. Bulgularımıza göre, 0.2 mg/ml etanol ekstresinde bulunan toplam flavonoid bileşen miktarı 44.465 ± 0.204 μg/ml ile kuersetine eşdeğer olarak bulunmuştur. Ayrıca bitki ekstresinin lipid peroksidasyonunu önleme aktivitesi, pozitif kontrol olan BHA (Beta hidroksi asit) ile kıyaslandığında A. schoenoprasum etkili olduğu görülmüştür. DPPH radikal söndürme aktivitesi en düşük ve en yüksek konsantrasyon değerleri, % 60,125 - 96,285 iken BHA için bu değerler % 82,178 - 97,357 olduğu bulunmuştur. A. schoenoprasum’un etanol ekstresinin, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Candida albicans gibi bazı patojen mikroorganizmalara karşı antimikrobiyal aktivite gösterdiği belirlenmiştir.

Anahtar Kelimeler

A. schoenoprasum L., , DPPH, , Lipid peroksidasyon, , Antimikrobiyal, , Flavonoid

Kaynakça

• Alam, MD. N., Bristi, N. J., & Rafiquzzaman, MD. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21: 143-52.
• Aslani, B. A., & Sirous G. (2016). Studies on oxidants and antioxidants with a brief glance at their relevance to the immune system. Life sciences, 146: 163-73. Atasoy, N. (2010). Van Bölgesinde Yetişen Endemik Bitkilerde Pro-Vitamin A (-Karoten) Tayini. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 15: 134-42.
• Barazani, O. N., Dudai, UR, K., & Goldhirsh, A. G. (2004). Cadmium accumulation in Allium schoenoprasum L. grown in an aqueous medium. Chemosphere, 57: 1213-18.
• Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181: 1199-200.
• Ceylan, Ş., Saral, Ö., Özcan, M., & Harşıt B. (2017). Yaban mersininin (Vaccinium myrtillus L.) farklı çözücü ekstraktlarındaki antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 18: 21-27.
• Chen, Shuxia, Shen, X., Cheng, S., Li, P., Du, J., Chang, Y., & Meng, H. (2013). Evaluation of garlic cultivars for polyphenolic content and antioxidant properties. PLoS One, 8(11), 1-12.
• Egert, M., & Tevini, M. 2002. Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environmental and Experimental Botany, 48: 43-49.
• El Shabrawy, M. O. A., Hosni, H.A., El Garf, İ. A., Marzouk, M. M., Kawashty, S. A., & Saleh, N. A. M., (2014). Flavonoids from Allium myrianthum Boiss. Biochemical Systematics and Ecology, 56: 125-28.
• Fırat, M. (2015). The Ethnobotanical Usage of Some East Anatolian (Turkey) Allium L. Species. Manas Journal of Agriculture Veterinary and Life Sciences, 5: 80-86.
• Haro, G., Sinaga, S. M., Iksen, I., Nerdy, N., & Theerachetmongkol, S. (2017). Protective effects of chives leaves (Allium schoenoprasum L.) infusion against ethylene glycol and ammonium chloride induced nephrolithiasis in rats. Journal of Applied Pharmaceutical Science, 7: 222-25.
• Lo, K. M., Cheung, C. K. (2005). Antioxidant activity of extracts from the fruiting bodies of Agrocybe aegerita var, Food Chemistry 89(4): 533–539.
• Losin, A., Raba, D. N., Moldovan, C., Popa, V. M., & Dumbravă, D. G. (2017). The influence of freezing on the content of vitamin C, chlorophylls and carotenoids in chives (Allium schoenoprasum l.). Scientific and Technical Bulletin: 49.
• Myint, A. A., Aregay, M. G., Kang, M., Kim, B.S., Lee, Y. W., & Kim, J. (2020). Comprehensive study on the formation mechanism of highly bioactive compounds from Allium hookeri root using subcritical water and their antioxidant and anticancer effects. The Journal of Supercritical Fluids, 157: 104709.
• Naeimi, A. F., & Alizadeh, M., (2017). Antioxidant properties of the flavonoid fisetin: An updated review of in vivo and in vitro studies. Trends in food science & technology, 70: 34-44.
• Ökmen, Gülten, Ali Arslan, Mustafa Vurkun, Mahabbat Mammadkhanli, and Olcay Ceylan. 2017. 'Farklı Baharatların Antimikrobiyal ve Antioksidan Aktiviteleri', Elektronik Mikrobiyoloji Dergisi TR, 15: 16-28.
• Parvu, A.E., Parvu, M., Vlase, L., Miclea, P., Mot, A.C., & Silaghi-Dumitrescu, R. (2014). Anti-inflammatory effects of Allium schoenoprasum L. leaves. J Physiol Pharmacol, 65: 309-15.
• Pokorny, J. (2001). Introduction, In: Antioxidants in Food Pratical Applications, Pokonry, J., Yanishlieva, N., Gordon, M. (Eds), Woodhead Publishing Limited, (pp 1-3) Cambridge, England: CRC Press.
• Sengupta, A., Ghosh, S., & Bhattacharjee, S. (2004). Allium vegetables in cancer prevention: an overview. Asian Pacific Journal of Cancer Prevention, 5: 237-45.
• Sharma, Om. P., & Bhat, T.K. (2009). DPPH antioxidant assay revisited. Food chemistry, 113: 1202-05.
• Shichiri, M., Yoshida, Y., & Niki, E. (2014). Chapter 4 - Unregulated Lipid Peroxidation in Neurological Dysfunction. In Ronald Ross Watson and Fabien De Meester (eds.), Omega-3 Fatty Acids in Brain and Neurological Health (Academic Press: Boston).
• Shirshova, T.I., Beshlei, I.V., Deryagina, V.P., Ryzhova, N. I., & Matistov, N.V. (2013). Chemical composition of Allium schoenoprasum leaves and inhibitory effect of their extract on tumor growth in mice. Pharmaceutical Chemistry Journal, 46: 672-75.
• Shrivastava, A., Chaturvedi, UP. MA., Singh, S. V., Kumar J., & Bhatia, G. (2012). A mechanism based pharmacological evaluation of efficacy of Allium sativum in regulation of dyslipidemia and oxidative stress in hyperlipidemic rats. Asian J Pharm Clin Res, 5: 123-26.
• Singh, V., Chauhan, G., Krishan, P., & Shri, R. (2018). Allium schoenoprasum L.: A review of phytochemistry, pharmacology and future directions. Natural product research, 32: 2202-16.
• Singh, V., Krishan, P., & Shri, R. (2018). Antioxidant-mediated neuroprotection by Allium schoenoprasum L. leaf extract against ischemia reperfusion-induced cerebral injury in mice. Journal of basic and clinical physiology and pharmacology, 29: 403-10.
• Stajner, D., Čanadanović‐Brunet, J., & Pavlović, A. (2004). Allium schoenoprasum L., as a natural antioxidant. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 18: 522-24.
• Stajner, D., & Popović, B. (2009). Comparative study of antioxidant capacity in organs of different Allium species. Open Life Sciences, 4: 224-28.
• Stajner, D., Popović, B. M., Ćalić‐Dragosavac, D., Malenčić, D., & Zdravković‐Korać, S. (2011). 'Comparative study on Allium schoenoprasum cultivated plant and Allium schoenoprasum tissue culture organs antioxidant status', Phytotherapy Research, 25: 1618-22.
• Stajner, D., & Varga, I. S. I. (2003). An evaluation of the antioxidant abilities of Allium species. Acta Biologica Szegediensis, 47: 103-06.
• Şapcı, H., & Vural, C. (2017). Echinops phaeocephalus (Asteraceae) Türünün Antimikrobiyal ve Antioksidan Aktivitesi. KSÜ Doğa Bilimleri Dergisi, 20: 355-60. Timite, G., Mitaine-Offer, A. C., Miyamoto, T., Tanaka, C., Mirjolet, J. M., Duchamp, O., et al. (2013). 'Structure and cytotoxicity of steroidal glycosides from Allium schoenoprasum', Phytochemistry, 88: 61-66.
• Trifunschi, S., Munteanu, M.F., Agotici, V., Pintea, S., & Gligor, R. (2015). Determination of flavonoid and polyphenol compounds in Viscum album and Allium Sativum extracts. International Current Pharmaceutical Journal, 4: 382-85.
• Wen, L., Zhao, Y., Jiang, Y., Yu, L., Zeng, X., Yang, J., et al. (2017). Identification of a flavonoid C-glycoside as potent antioxidant. Free Radical Biology and Medicine, 110: 92-101.
• Zhishen, J. Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food chemistry, 64: 555-59.