Effects of Endophytic Bacteria on Some Physiological Traits and Nutrient Contents in Pepper Seedlings under Drought Stress

Abstract

The present study was conducted to determine the effects of the endophytic bacteria (EB) on some physiological traits and nutrient contents in pepper (Capsicum annuum L.) seedlings grown under drought stress. The pepper cv. Mostar F1 and two EB isolates [Bacillus thurigiensis (CA41/1) and Ochrobactrum sp. (CB36/1)] were employed under drought stress condition. The first EB application was at a density of 108 CFU mL-1 as 10 mL plant-1 at the first cotyledon leaf stage and the second one was two weeks later. The seedlings were irrigated by gravimetric method on a regular basis every two days. Twenty days after EB application, irrigation was terminated completely in half of the applications in order to form drought stress for 7 days. Among the studied traits, membrane damage index, leaf relative water content, amount of malondialdehyde, catalase enzyme activity, ascorbate peroxidase enzyme activity, and the contents of some mineral elements (K, Ca and Mg) were significantly different in drought stressed seedlings compared the control (regularly irrigated) seedlings. EB (Especially CA41/1) had generally positive effects on most studied traits, whereas drought stress had generally negative effects on the mentioned traits. There might be a high potential of EB fighting against drought stress in pepper; however, one keeps in mind that there is variation in the performance of EB; therefore, the best EB combinations have to be determined even for cultivars in each plant species in future studies.

Keywords

• drought
• EBs
• Enzyme activity
• Growth
• Mineral matters

Endofitik Bakterilerin Kuraklık Stresi Altındaki Biber Fidelerinin Bazı Fizyolojik Özellikleri ve Besin İçerikleri Üzerine Etkileri

Abstract

Bu çalışma, kuraklık stresi altında yetiştirilen biber (Capsicum annuum L.) fidelerinde endofitik bakterilerin (EB) bazı fizyolojik özellikler ve besin içerikleri üzerine etkilerini belirlemek amacıyla yapılmıştır. Mostar F1 Biber çeşidi, iki EB izolatı [Bacillus thurigiensis (CA41 / 1) ve Ochrobactrum sp. (CB36 / 1)] kuraklık stresi altında kullanılmıştır. İlk EB uygulaması, ilk kotiledon yaprağı aşamasında 10 mL/bitki olarak 108 CFU/mL yoğunlukta ve ikincisi ise iki hafta sonra yapılmıştır. Fideler, iki günde bir düzenli olarak gravimetrik yöntemle sulanmıştır. EB uygulamasından yirmi gün sonra, 7 günlük kuraklık stresi oluşturmak için uygulamaların yarısında sulamaya tamamen son verilmiştir. Çalışılan özellikler arasında, kuraklık stresi altındaki fidelerde kontrol (düzenli olarak sulanan) fideler ile karşılaştırıldığında, membran zararlanma indeksi, yaprak oransal su içeriği, malondialdehit miktarı, katalaz enzim aktivitesi, askorbat peroksidaz enzim aktivitesi ve bazı mineral elementlerin içerikleri (K, Ca ve Mg) önemli ölçüde farklı bulunmuştur. EB'ler (Özellikle CA41 / 1), incelenen özelliklerin çoğunda genel olarak olumlu etkilere sahipken, kuraklık stresi belirtilen özellikler üzerinde genellikle olumsuz etkilere yol açmıştır. Biberde kuraklık stresine karşı savaşan yüksek bir EB potansiyeli olabilir; ancak, EB'lerin performansında farklılıklar olduğu unutulmamalıdır; bu nedenle, en iyi EB kombinasyonları, gelecekteki çalışmalarda her bitki türündeki çeşitler için belirlenmelidir.

Keywords

• biber
• EBs
• Enzim aktivitesi
• Büyüme
• Kuraklık
• Mineral maddeler

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