Porphyridium cruentum’un Kapalı Alan Tübüler Fotobiyoreaktör Kültüründe Fikoeritrin Birikimi

Öz

Mikroalgler, akuakültür ile birlikte farmasötik, yem, gıda, tarım ve enerji gibi endüstriyel alanlarda kullanılmaktadır. Mikroalg pigmentleri potansiyel gıda renklendirme ajanı olmakla birlikte, yapılarında çoklu doymamış yağ asitleri gibi biyoaktif bileşenler içermektedir. Porphyridium cruentum fikoeritrin, klorofil ve karotenoidler gibi değerli pigmentleri biriktirebilme yeteneğine sahip kırmızı bir alg olarak bilinmektedir. Bu çalışmada, P. cruentum’un 51 gün boyunca kapalı alanda pilot ölçekli tübüler fotobiyoreaktörde kültürü yapılmıştır. En yüksek hücre sayısı 31.84 x 106 hücre mL-1 olarak belirlenmiş olup, en yüksek spesifik büyüme oranı 0.80 olarak belirlenmiştir. Toplam fikobiliprotein ve fikoeritrin miktarları erken üssel fazda sırasıyla 0.252 ± 0.009 mg mL-1 ve 0.224 ± 0.007 mg mL-1’ye ulaşmıştır.

Anahtar Kelimeler

• Fikoeritrin
• Pigment
• Porphyridium cruentum
• Tübüler fotobiyoreaktör

Phycoerythrin Accumulation of Porphyridium cruentum Culture at Indoor Tubular Photobioreactor

Abstract

Microalgae are used in aquaculture and various industrial fields such as pharmaceuticals, feed, food, agriculture, and energy. Microalgae is a potential natural food coloring agent as pigments and contain bioactive components such as polyunsaturated fatty acids (PUFA) in their composition. Porphyridium cruentum is a red alga with the ability to accumulate valuable pigments biomolecules such as phycoerythrin (PE), chlorophyll, and other carotenoids. In this study, P. cruentum was cultured for 51 days at the indoor pilot scale tubular photobioreactor (PBR). The highest cell number was 31.84 x 106 cells mL-1 and the highest specific growth rate was determined as 0.80. Total phycobiliprotein and phycoerythrin amounts were reached 0.252 ± 0.009 mg mL-1 and 0.224 ± 0.007 mg mL-1 at the early exponential phase, respectively.

Keywords

• Phycoerythrin
• Porphyridium cruentum
• pigment
• tubular photobioreactor

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