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Effect of Fusarium Head Blight on Total Protein and Carbonhydrate Contents of Wheat

Yıl 2013, Cilt: 23 Sayı: 2, 149 - 153, 01.06.2013

Öz

Fusarium culmorum is one of the pathogens causing Fusarium head blight (FHB) in winter wheat. The aim of this study was to elucidate the effects of the head scab on its yield as well as total carbohydrate and protein content. Four different wheat varieties (Saggittario, Tosunbey, Golia and Yunak) and a DON chemotype of the fungus were employed in the research. Heads were syringeinoculated with conidial suspension of the fungus during wheat anthesis. Disease severity was assessed by measuring the diseased spikelet relative to the whole head. Carbohydrates and protein contents in Fusarium-damaged and healthy kernels were analyzed using Anthron and Kjeldahl method, respectively. Disease severity differed greatly among varieties, resulting reduced a thousand kernel weight (TKW). Protein contents did not changed in FHB infested kernels compared to control parcels, although carbohydrate ration reduced in FHB infested kernels of two of the varieties compared to control parcels.

Kaynakça

  • Baht CV, Beedu R, Ramakrishna Y, Munshi RL (1989). Outbreak of trichothecene toxicosis associated with consumption of mould-damaged wheat products in Kashmir Valley, India. Lancet, p. 35-37. Bai G (1995). Scab of wheat: epidemiology, inheritance of resistance and molecular markers linked to cultivar resistance. Ph.D. thesis. West Lafayette, IN, USA, Purdue University.
  • Bechtel DB, Kaleikau LA, Gaines RL, Seitz LM (1985). The Effects of Fusarium graminearum infection on wheat kernels. Cereal Chemistry, 62: 191–197.
  • Boyacıoğlu D, Hettiarachchy NS (1995). hanges in some biochemical components of wheat grain that was infected with Fusarium graminearum Journal of Cereal Science Volume 21, Issue 1, January 1995, 57–62.
  • Chrpová J, Šíp V, Štočková L, Milec Z, Bobková L (2010). Resistance of Winter Wheat Varieties Registered in the Czech Republic to Fusarium Head Blight in Relation to the Presence of Specific Rht Alleles. Czech Journal of Genetics and Plant Breeding, 46 (3): 122-134.
  • Dexter JE, Marchylo BA, Clear RM, Clarke JM (1997). Effect of Fusarium head blight on semolina milling and pasta-making quality of durum wheat. Cereal Chem. 74(5):519–525.
  • Dubin HJ, Gilchrist L, Reeves J, McNab A (1997). Fusarium head scab: global status and future prospects. Mexico, DF, CIMMYT.
  • Gerhardt P, Murray RGE, Wood WA, Krieg NR (1994). Methods for General and Molecular Bacteriology, ASM, Washington DC. ISBN 1-55581-048-9:518
  • Gilchrist L, Rajaram S, Mujeeb-Kazi A, van Ginkel M, Vivar H, Pfeiffer,, W (1997). In H.J. Dubin, L. Gilchrist, J. Reeves & A. McNab, eds. Fusarium head scab: global status and future prospects. Mexico, DF, CIMMYT.
  • ICC (1980). ICC Standard No: 105/1. Method for the Determination of Crude Protein in Cereals and Cereal Products for Food and for Feed. Standard Methods of the International Association for Cereal Chemistry (ICC). Verlag Moritz Schafer. Detmold
  • Ireta MJ; Gilchrist L (1994). Fusarium head scab of wheat (Fusarium graminearum Schwabe). Wheat Special Report No. 21b. Mexico, DF, CIMMYT.
  • Işık V (2012). Fusarium culmorum’un Farklı Buğday Çeşitlerinde Zearalenone Üretimi ve Hastalık Şiddetiyle Korelasyonunun Saptanması. YL tezi, ÇOMÜ, Fen Bilimleri Enstitüsü, Çanakkale
  • Khalil IH, Carver BF, Krenzer EG, MacKown CT, Horn GW (2002). Genetic trends in winter wheat yield and test weight under dualpurpose and grain only management. Crop Sci. 710-715.
  • Luo XY (1988). Fusarium toxin contamination in China. Proc. Japan Assoc. Mycotoxicology Suppl., 1: 97Marasas WFO, Jaskiewickz K, Venter FS Schalkwyk DJ (1988). Fusarium moniliforme contamination of maize in oesophageal cancer areas in Transkei. S. AF. Med. J., 74: 110-114.
  • Miedaner T, Schneider B, Geiger HH (2003). Deoxynivalenol (DON) Content and Fusarium Head Blight Resistance in Segregating Populations of Winter Rye and Winter Wheat. Crop Science, 43 (2): 519-5
  • Miedaner T, Schilling AG, Geiger HH (2004). Competition effects among isolates of Fusarium culmorum differing in aggressiveness and mycotoxin production on heads of winter rye. European Journal of Plant Pathology 110:63–70.
  • Nightingale M, Marchylo BA, Clear RM, Dexter JE, Preston KR (1999). Fusarium head blight: Effect of fungal protease on wheat storage protein. Cereal Chem., 76, 150-158.
  • SAS 1999. SAS V8 User Manual, SAS Institute, Cary NC.
  • Snidjers CHA (1989). Current status of breeding wheat for Fusarium head blight resistant and mycotoxin problem in the Netherlands. Foundation of Agricultural Plant Breeding, Wageningen. In M.M. Kohli, ed. Taller sobre la Fusariosis de la espiga en America del Sur. Mexico, DF, CIMMYT.
  • Snijders CHA Perkowski J (1990). Effects of Head Blight Caused by Fusarium culmorum on Toxin Content and Weight of Wheat Kernels. Phytopathology, 80: 566–570.
  • Tunalı B, Büyük O, Erdurmuş D, Ozseven I, Demirci A (2006). Fusarium Head Blight and Deoxynivalenol Accumulation of Wheat in Marmara Region and Reactions of Wheat Cultivars and Lines to F. graminearum and F.culmorum. J. Plant Pathol., 5(2): 150-156.
  • Wilcoxson RD, Busch RH, Ozmon EA (1992). Fusarium Head Blight Resistance in Spring Wheat Cultivars. Plant Disease, 76 (7): 658–661.

Fusarium Başak Yanıklığının Buğdayda Toplam Protein ve Karbonhidrat İçeriğine Etkisi

Yıl 2013, Cilt: 23 Sayı: 2, 149 - 153, 01.06.2013

Öz

Fusarium culmorum Fusarium başak yanıklığına (Fusarium head blight; FHB) sebep olan önemli bir fungustur. Bu çalışmanın amacı F. culmorum ile enfeksiyon sonucu verime ve protein ile karbonhidrat içeriğine olan etkisini araştırmaktır. Çalışmada 4 buğday çeşidi (Saggittario, Tosunbey, Golia ve Yunak) ve fungusun virülensliği tespit edilmiş bir izolatı kullanılmıştır. Fungusun spor süspansiyonu çiçeklenme döneminde başaklara şırınga ile enfekte edilmiştir. Hastalık şiddeti hastalıklı başakçık alanının tüm başağa oranı alınarak hesaplanmıştır. Hastalıklı ve sağlıklı tanelerde karbonhidrat ve protein içeriği sırasıyla Anthron ve Kjeldahl metodu kullanılarak saptanmıştır. Hastalık şiddeti çeşitler arasında büyük oranda farklılıklar göstermiş ve başak enfeksiyonu bin dane ağırlığının (BDA) düşmesini sonuçlamıştır. FHB ile bulaşık danelerde, kontrollere kıyasla protein oranı açısından bir farklılık oluşmadığı, fakat iki çeşitte karbonhidrat içeriğinin FHB ile bulaşık tohumlarda istatistiki olarak düştüğü görülmüştür.

Kaynakça

  • Baht CV, Beedu R, Ramakrishna Y, Munshi RL (1989). Outbreak of trichothecene toxicosis associated with consumption of mould-damaged wheat products in Kashmir Valley, India. Lancet, p. 35-37. Bai G (1995). Scab of wheat: epidemiology, inheritance of resistance and molecular markers linked to cultivar resistance. Ph.D. thesis. West Lafayette, IN, USA, Purdue University.
  • Bechtel DB, Kaleikau LA, Gaines RL, Seitz LM (1985). The Effects of Fusarium graminearum infection on wheat kernels. Cereal Chemistry, 62: 191–197.
  • Boyacıoğlu D, Hettiarachchy NS (1995). hanges in some biochemical components of wheat grain that was infected with Fusarium graminearum Journal of Cereal Science Volume 21, Issue 1, January 1995, 57–62.
  • Chrpová J, Šíp V, Štočková L, Milec Z, Bobková L (2010). Resistance of Winter Wheat Varieties Registered in the Czech Republic to Fusarium Head Blight in Relation to the Presence of Specific Rht Alleles. Czech Journal of Genetics and Plant Breeding, 46 (3): 122-134.
  • Dexter JE, Marchylo BA, Clear RM, Clarke JM (1997). Effect of Fusarium head blight on semolina milling and pasta-making quality of durum wheat. Cereal Chem. 74(5):519–525.
  • Dubin HJ, Gilchrist L, Reeves J, McNab A (1997). Fusarium head scab: global status and future prospects. Mexico, DF, CIMMYT.
  • Gerhardt P, Murray RGE, Wood WA, Krieg NR (1994). Methods for General and Molecular Bacteriology, ASM, Washington DC. ISBN 1-55581-048-9:518
  • Gilchrist L, Rajaram S, Mujeeb-Kazi A, van Ginkel M, Vivar H, Pfeiffer,, W (1997). In H.J. Dubin, L. Gilchrist, J. Reeves & A. McNab, eds. Fusarium head scab: global status and future prospects. Mexico, DF, CIMMYT.
  • ICC (1980). ICC Standard No: 105/1. Method for the Determination of Crude Protein in Cereals and Cereal Products for Food and for Feed. Standard Methods of the International Association for Cereal Chemistry (ICC). Verlag Moritz Schafer. Detmold
  • Ireta MJ; Gilchrist L (1994). Fusarium head scab of wheat (Fusarium graminearum Schwabe). Wheat Special Report No. 21b. Mexico, DF, CIMMYT.
  • Işık V (2012). Fusarium culmorum’un Farklı Buğday Çeşitlerinde Zearalenone Üretimi ve Hastalık Şiddetiyle Korelasyonunun Saptanması. YL tezi, ÇOMÜ, Fen Bilimleri Enstitüsü, Çanakkale
  • Khalil IH, Carver BF, Krenzer EG, MacKown CT, Horn GW (2002). Genetic trends in winter wheat yield and test weight under dualpurpose and grain only management. Crop Sci. 710-715.
  • Luo XY (1988). Fusarium toxin contamination in China. Proc. Japan Assoc. Mycotoxicology Suppl., 1: 97Marasas WFO, Jaskiewickz K, Venter FS Schalkwyk DJ (1988). Fusarium moniliforme contamination of maize in oesophageal cancer areas in Transkei. S. AF. Med. J., 74: 110-114.
  • Miedaner T, Schneider B, Geiger HH (2003). Deoxynivalenol (DON) Content and Fusarium Head Blight Resistance in Segregating Populations of Winter Rye and Winter Wheat. Crop Science, 43 (2): 519-5
  • Miedaner T, Schilling AG, Geiger HH (2004). Competition effects among isolates of Fusarium culmorum differing in aggressiveness and mycotoxin production on heads of winter rye. European Journal of Plant Pathology 110:63–70.
  • Nightingale M, Marchylo BA, Clear RM, Dexter JE, Preston KR (1999). Fusarium head blight: Effect of fungal protease on wheat storage protein. Cereal Chem., 76, 150-158.
  • SAS 1999. SAS V8 User Manual, SAS Institute, Cary NC.
  • Snidjers CHA (1989). Current status of breeding wheat for Fusarium head blight resistant and mycotoxin problem in the Netherlands. Foundation of Agricultural Plant Breeding, Wageningen. In M.M. Kohli, ed. Taller sobre la Fusariosis de la espiga en America del Sur. Mexico, DF, CIMMYT.
  • Snijders CHA Perkowski J (1990). Effects of Head Blight Caused by Fusarium culmorum on Toxin Content and Weight of Wheat Kernels. Phytopathology, 80: 566–570.
  • Tunalı B, Büyük O, Erdurmuş D, Ozseven I, Demirci A (2006). Fusarium Head Blight and Deoxynivalenol Accumulation of Wheat in Marmara Region and Reactions of Wheat Cultivars and Lines to F. graminearum and F.culmorum. J. Plant Pathol., 5(2): 150-156.
  • Wilcoxson RD, Busch RH, Ozmon EA (1992). Fusarium Head Blight Resistance in Spring Wheat Cultivars. Plant Disease, 76 (7): 658–661.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

F. Mert-türk Bu kişi benim

F. Kahriman Bu kişi benim

R. Gencer Bu kişi benim

C. O. Egesel Bu kişi benim

Yayımlanma Tarihi 1 Haziran 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 23 Sayı: 2

Kaynak Göster

APA Mert-türk, F., Kahriman, F., Gencer, R., Egesel, C. O. (2013). Fusarium Başak Yanıklığının Buğdayda Toplam Protein ve Karbonhidrat İçeriğine Etkisi. Yuzuncu Yıl University Journal of Agricultural Sciences, 23(2), 149-153.

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