Micropropagation of Endemic Astragalus trojanus Stev. with Nodal Culture

Yıl 2017, Cilt: 27 Sayı: 2, 268 - 275, 30.06.2017

Emine Atalay , Münüre Tanur Erkoyuncu , Semiha Erişen , Mustafa Yorgancılar

https://doi.org/10.29133/yyutbd.268674

Cited By: 1

Öz

The clonal propagation with
nodal cultures of the medicinal endemic plant species Astragalus trojanus Stev. was aimed in this study. Seeds were
germinated in Murashige and Skoog (MS) medium, and the nodal segments obtained
from seedlings were used as an explant. Firstly, the effects of MS and (Woody
Plant Medium) WPM nutrient media on shoot multiplication were investigated. The
explants were cultured in MS or WPM nutrient media containing at different
concentrations (0.2, 0.5, 1.0, 2.0 mg L^-1) 6-benzyl amino purine
(BAP). After 4 weeks from culture, the highest number of
shoots (2.80 number/explant) was obtained in WPM medium containing 0.5 mg L^-1
BAP. Nodal segments were cultured in WPM medium containing different
concentrations of BAP, thidiazuron (TDZ), kinetin (KIN) (0.2, 0.5, 1.0, 2.0 mg L^-1)
to determine the effect of cytokinin source and concentrations on shoot
multiplication. At the end of 4 weeks, it was determined that TDZ and KIN had
similar effect on number of shoots. The highest number of shoots (2.09
number/explant) was obtained from WPM media containing BAP. Regenerated shoots
were transferred to WPM media containing 1 or 3 mg L^-1
indole-3-butyric acid (IBA), naphtalene acetic acid (NAA) or indole-3-acetic
acid (IAA) for rooting. After 4 weeks from culture, the most root number (4.33
number/explant) were obtained from the WPM medium containing 3 mg L^-1
IAA, while the highest rooting percentage was obtained from WPM medium
containing 3 mg L^-1 IBA with 80%. Rooted seedlings were transferred
to plastic cups containing peat and perlite in a ratio of 1:1 and successfully
acclimatized. Plant culture was completed at 15 weeks. The effective clonal
propagation method was described for the endemic species Astragalus trojanus Stev.

Anahtar Kelimeler

Astragalus, Clonal propagation, Rooting, Nodal segment, WPM

Endemik Astragalus trojanus Stev’in Nodal Kültür ile Mikroçoğaltımı

Öz

Bu araştırmada, tıbbi özelliklere sahip endemik Astragalus
trojanus Stev. bitkisinin nodal kültürler ile klonal çoğaltımı
amaçlanmıştır. Tohumlar Murashige ve Skoog (MS) besin ortamında çimlendirilmiş
ve elde edilen steril fidelerin nodal segmentleri eksplant olarak
kullanılmıştır. İlk olarak MS ve Woody Plant Medium (WPM) besin ortamlarının
sürgün çoğaltımına etkileri araştırılmıştır. Eksplantlar farklı
konsantrasyonlarda (0.2, 0.5, 1.0, 2.0 mg/L) 6-benzil amino purin (BAP) içeren
MS ya da WPM besin ortamlarında kültüre alınmıştır. Kültürden 4 hafta sonra en
fazla sürgün sayısı (2.80 adet/eksplant) 0.5 mg/L BAP içeren WPM ortamında elde
edilmiştir. Çalışmanın devamında nodal segmentler, sitokinin kaynağı ve
konsantrasyonlarının sürgün çoğaltımına etkisini belirlemek amacıyla BAP,
thidiazuron (TDZ), kinetin (KIN)’in farklı konsantrasyonlarını (0.2, 0.5, 1.0,
2.0 mg/L) içeren WPM temel besin ortamında kültüre alınmıştır. 4 hafta sonunda
elde edilen sürgün sayıları bakımından TDZ ve KIN’in benzer etki gösterdiği, en
yüksek sürgün sayısının (2.09 adet/eksplant) BAP’dan elde edildiği tespit
edilmiştir. Rejenere sürgünler köklenme için 1 veya 3 mg/L indol-3-butirik asit
(IBA), naftalen asetik asit (NAA) veya indol-3-asetik asit (IAA) içeren WPM
ortamlarına aktarılmıştır. Kültürden 4 hafta sonra en yüksek köklenme yüzdesi
(%80) 3 mg/L IBA içeren WPM ortamından elde edilirken, en fazla kök sayısı
(4.33 adet/eskplant) 3 mg/L IAA içeren WPM ortamından elde edilmiştir. Köklenen
bitkicikler 1:1 oranında torf ve perlit içeren plastik bardaklara aktarılarak
dış ortama başarıyla alıştırılmış ve tam bitki gelişimi 15 hafta sonunda
tamamlanmıştır. Bu araştırma ile, Astragalus trojanus Stev. endemik
türünde etkili bir klonal çoğaltım metodu tanımlanmıştır.

Anahtar Kelimeler

Astragalus, Klonal çoğaltım, Köklenme, Nodal segment, WPM

Kaynakça

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