Determination of Quality Status of Persimmon Pollen Stored at Low Temperatures

Year 2020, Volume: 30 Issue: 2, 345 - 354, 30.06.2020

Akide Özcan , Şakir Burak Bükücü

https://doi.org/10.29133/yyutbd.667521

Cited By: 1

Abstract

This study was carried out to determine the pollen quality status of 3 foreign pollinator and 2 native Persimmons (Diospyros kaki L.) genotypes at different storage temperatures for 1 year. Pollen was stored at +4 °C, 0 °C , -20 °C and -80 °C for 1 year, rate of viability and germination were determined every 4 months. 2, 3, 5 Triphenyltetrazolium Chloride (TTC) solution was used to determine the viability of the pollen, and agar in petri dish method was used to determine the germination rate of the pollen. The initial measurement of the genotypes ranged from 58.12% to 76.65% in pollen viability rates. At the end of the 12th month, the highest pollen viability at +4 °C, 0 °C, -20 °C, and -80 °C was determined to be 13.00 %, 42.01 %, 60.72 %, and 59.45 %, respectively. The pollen germination rates of the genotypes were found to be between 52.84 % and 67.49 % in the initial measurement. At the end of the 12th month, the highest germination rates were determined as 1% at +4 °C, 13.24 % at 0 °C, 23.87 % at -20 °C, and 24.99 % at -80 °C. At the end of the storage period, pollination potentials of genotypes were found to be inadequate at +4 °C and 0 °C, and relatively acceptable at pollination ability at -20 °C and -80 °C.

Keywords

Persimmon, Pollen, Viability, Germination, Low Temperatures

Düşük Sıcaklıklarda Depolanan Trabzon Hurması Çiçek Tozlarının Kalite Durumlarının Belirlenmesi

Abstract

Bu çalışma, 3 yabancı tozlayıcı çeşit ve 2 yerli Trabzon hurması (Diospyros kaki L.) genotipine ait çiçek tozlarının farklı depo sıcaklıklarında 1 yıl süre ile saklanması sonunda kalite durumlarının belirlenmesi amacıyla yapılmıştır. Çiçek tozları +4 °C, 0 °C, -20 °C ve -80 °C ’de 1 yıl süre ile saklanmış ve 4 ayda bir canlılık ve çimlenme durumları belirlenmiştir. Çiçek tozu canlılığının tespitinde 2, 3, 5 Triphenyl Tetrazolium Clorid (TTC) çözeltisi, çiçek tozlarının çimlenme oranlarının belirlenmesinde ise petride agar yöntemi kullanılmıştır. Genotiplerin ilk ölçüm çiçek tozu canlılık oranları % 58.12 ile % 76.65 değerleri arasında değişmektedir. Son ölçüm olan 12. ayın sonunda +4 °C, 0 °C , -20 °C ve -80 °C sıcaklıklarda en yüksek çiçek tozu canlılık oranlarının sırasıyla %13.00, %42.01, % 60.72 ve % 59.45 olduğu belirlenmiştir. Genotiplere ait çiçek tozlarının çimlenme oranları ise ilk ölçümde % 52.84 ile % 67.49 değerleri arasında olduğu tespit edilmiştir. 12. ayın sonunda en yüksek çiçek tozu çimlenme oranlarının +4 °C ’de % 1, 0 °C ’de % 13.24, -20 °C ’de % 23.87 ve -80 °C ’de ise % 24.99 olarak belirlenmiştir. Depolama süresi sonunda genotiplerin tozlayıcılık potansiyellerinin, +4 °C ve 0 °C’lerde yetersiz, -20 °C ve -80 °C’lerde ise tozlayıcılık yeteneği yönüyle nispeten kabul edilebilir düzeyde olduğu tespit edilmiştir.

Keywords

Trabzon hurması, Çiçek tozu, Canlılık, Çimlenme, Düşük sıcaklık

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