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Characterization and Haplotype Analysis of Colletotrichum truncatum in Greenhouse Tomato in Turkey

Yıl 2021, Cilt: 31 Sayı: 3, 776 - 785, 15.09.2021
https://doi.org/10.29133/yyutbd.912293

Öz

Anthracnose caused by Colletotrichum truncatum (Schwein.) Andrus and W.D. Moore, is an economically important disease of most tropical crops. In recent years, it has been reported that it is also pathogenic on tomatoes. In this study, the infected fruits were obtained from Antalya province in 2019. Isolates were purified by taking single spore. Conidia were measured as 22.5-32.5 × 3.75 µm. DNA isolation was carried out using the CTAB method. After the PCR amplification, the PCR product was run on agarose gel, visualized with a UV transilluminator, and sequenced. Phylogenetic analysis was conducted in MEGA 7. Based on morphological and phylogenetic analysis, the CT isolate was identified as C. truncatum. Pathogenicity tests were carried out using tomato leaves and cherry tomatoes. The inoculated leaves and tomatoes were incubated on a moist filter paper in climate chambers under 27 °C temperature and 12:12 h light-dark conditions. Acervuli were observed on infected tissues on the 7th day of inoculation. Haplotype, the number of haplotypes, and nucleotide diversity were analyzed by DnaSP 6.0 software. 8 haplotypes were determined according to the ITS sequence of 46 C. truncatum isolates from different countries. The median-joining network analysis of haplotypes was drawn using the NETWORK 10 program. It was determined that the CT isolate reported with this study from Turkey and the other reference isolates reported on tomatoes were in the H1 which is the most common haplotype.

Kaynakça

  • Almaraz Sánchez, A., Ayala Escobar, V., Landero Valenzuela, N., Tlatilpa Santamaría, I. F., & Nieto Angel, D. (2019). First Report of Colletotrichum truncatum of Solanum lycopersicum in Mexico. Plant Disease 103 (7). doi: 10.1094/PDIS-10-18-1809-PDN
  • Anonymous (2017). https://www.seminis-us.com/resources/disease-guides/tomatoes/anthracnose-2/. Accession date: 15.02.21
  • Belov, G. L., Belosokhov, A. F., Kutuzova, I. A., Statsyuk, N. V., Chudinova, E. M., Alexandrova, A. V., Kokaeva, L. Y., & Elansky, S. N. (2018). Colletotrichum coccodes in potato and tomato leaves in Russia. Journal of Plant Diseases and Protection 125: 311–317. doi:10.1007/s41348-017-0138-0
  • Carbone, I., & Kohn, L. (2004). Inferring process from pattern in fungal population genetics. In: Khachatourians, G. G., Arora, D. K. (eds). Fungal Genomics, vol. 4. Elsevier Science B.V, Amsterdam
  • Damm, U., Woudenberg, J. H. C., Cannon, P. F., & Crous, P. W. (2009). Colletotrichum species with curved conidia from herbaceous hosts. Fungal Diversity 39: 45–87.
  • Damm, U., Cannon, P. F., Woudenberg, J. H., Johnston, P. R., Weir, B. S., Tan, Y. P., Shivas, R. G., & Crous, P. W. (2012). The Colletotrichum boninense species complex. Studies in Mycology 73 (1): 1–36. doi:10.3114/sim0002
  • da Silva, L. L., Moreno, H. L. A., Correia, H. L. N., Santana, M. F., & de Queiroz, M. V. (2020). Colletotrichum: species complexes, lifestyle, and peculiarities of some sources of genetic variability. Applied Microbiology and Biotechnology 104(5):1891-1904.
  • Diao, Y. Z., Zhang, C., Lin, D., & Liu, X. L. (2014). First report of Colletotrichum truncatum causing anthracnose of tomato in China. Plant Disease 98 (5): 687. doi: 10.1094/PDIS-05-13-0491-PDN
  • FAO (2020). Production quantities of tomatoes by country, 1994-2018. http://www.fao.org/faostat/en/#data/QC/visualize
  • He, Y., Chen, Q., Shu, C., Yang, M., & Zhou, E. (2016). Colletotrichum truncatum, a new cause of anthracnose on Chinese flowering cabbage (Brassica parachinensis) in China. Tropical Plant Pathology 41: 183–192. doi:10.1007/s40858-016-0086-4
  • Isaac, S. (1992). Fungal Plant Interaction. Chapman and Hall Press, London, 115 p. Jayawardena, R. S., Hyde, K., Damm, U., Cai, L., Liu, M., Li, X., Zhang, W., Zhao, W., & Yan, J. (2016) Notes on currently accepted species of Colletotrichum. Mycosphere 7(8):1192–1260. https://doi.org/10. 5943/mycosphere/si/2c/9
  • Katoch, A., Prabhakar, C. S., & Sharma, P. N. (2016). Metageographic population analysis of Colletotrichum truncatum associated with chili fruit rot and other hosts using ITS region nucleotide sequences. Journal of Plant Biochemistry and Biotechnology 25 (1): 64–72. doi: 10.1007/s13562-015-0310-1
  • Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111-120.
  • Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA 7: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35: 1547-1549.
  • Lefort, F., Lally, M., Thompson, D., & Douglas, G. C. (1998). Morphological traits microsatellite fingerprinting and genetic relatedness of a stand of elite oaks (Q. robur L.) at Tuallynally, Ireland. Silvae Genetica 47: 5-6.
  • Marcelino, J., Giordano, R., Gouli, S., Gouli, V., Parker, B L., Skinner, M., TeBeest, D., & Cesnik, R. (2008). Colletotrichum acutatum var. fioriniae (teleomorph: Glomerella acutata var. fioriniae var. nov. infection of a scale insect. Mycologia 100 (3): 353–374.
  • Marcelino, J. A., Gouli, S., Parker, B. L., Skinner, M. & Giordano, R. (2009). Entomopathogenic activity of a variety of the fungus, Colletotrichum acutatum, recovered from the elongate hemlock scale, Fiorinia externa. Journal of Insect Science 9: 13. doi:10.1673/031.009.1301
  • Posada, D., & Crandall, K. A. (2001). Intraspecific gene genealogies: trees grafting into networks. Trends in Ecology & Evolution 1: 37–45.
  • Ranathunge, N. P., & Sandani, H. B. P. (2016). Deceptive behaviour of Colletotrichum truncatum: strategic survival as an asymptomatic endophyte on non-host species. Journal of Plant Protection Research 56 (2): 157-162. doi:10.1515/jppr-2016-0026
  • Rao, S., Sharda, S., Oddi, V., & Nandineni, M. R. (2018). The landscape of repetitive elements in the refined genome of Chilli anthracnose fungus Colletotrichum truncatum. Frontiers in Microbiology 9, 2367. doi:10.3389/fmicb.2018.02367
  • Rogério, F., Ciampi-Guillardi, M., Barbieri, M. C., Bragança, C. A., Seixas, C. D., Almeida, A. M., & Massola, N. S. (2017). Phylogeny and variability of Colletotrichum truncatum associated with soybean anthracnose in Brazil. Journal of Applied Microbiology 122 (2): 402-415. doi: 10.1111/jam.13346. PMID: 27859958
  • Saini, T. J., Gupta, S. G., & Anandalakshmi, R. (2017). Detection of tomato anthracnose caused by Colletotrichum truncatum in India. Australasian Plant Disease Notes 12: 48. doi:10.1007/s13314-017-0271-4
  • Shivaprakash, M. R., Appannanavar, S. B., Dhaliwal, M., Gupta, A., Gupta, S., Gupta, A., & Chakrabarti, A. (2011). Colletotrichum truncatum: an unusual pathogen causing mycotic keratitis and endophthalmitis. Journal of Clinical Microbiology 49 (8): 2894–2898. doi:10.1128/JCM.00151-11
  • Torres-Calzada, C., Tapia-Tussell, R., Higuera-Ciapara, I., Huchin-Poot, E., Martin-Mex, R., Nexticapan-Garcez, A., & Perez-Brito, D. (2018). Characterization of Colletotrichum truncatum from papaya, pepper and physic nut based on phylogeny, morphology and pathogenicity. Plant Pathology 67 (4): 821-830.
  • Villafana, R. T., Ramdass, A. C., & Rampersad, S. N. (2018). First report of Colletotrichum truncatum causing anthracnose in tomato fruit in Trinidad. Plant Disease 102 (9): 1857. doi:10.1094/PDIS-02-18-0319-PDN
  • White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M. A., Gelfand, D. H., Sninsky, J. J., & White, T. J. (eds). PCR Protocols: A Guide to Methods and Applications Academic Press, New York, pp 315-322. http://dx.doi.org/10.1016/B978-0-12-372180-8.50042-1
  • Wikee, S., Cai, L., Pairin, N., McKenzie, E. H. C., Su, Y. Y., Chukeatirote, E., Thi, H. N., Bahkali, A. H., Moslem, M. A., Abdelsalam, K., & Hyde, K. D. (2011). Colletotrichum species from Jasmine (Jasminum sambac). Fungal Diversity 46, 171–182

Türkiye'de Sera Domatesinde Colletotrichum truncatum’un Karakterizasyonu ve Haplotip Analizi

Yıl 2021, Cilt: 31 Sayı: 3, 776 - 785, 15.09.2021
https://doi.org/10.29133/yyutbd.912293

Öz

C. truncatum'un (Schwein.) Andrus and W.D. Moore neden olduğu antraknoz çoğu tropikal ürünün ekonomik olarak önemli bir hastalığıdır. Son yıllarda domateste de patojen olduğu rapor edilmiştir. Bu çalışmada, enfekteli meyveler 2019 yılında Antalya ilinden elde edilmiştir. İzolatlar tek spor alınarak saflaştırıldı. Konidiler 22.5-32.5 × 3.75 µm olarak ölçülmüştür. DNA izolasyonu CTAB metodu kullanılarak gerçekleştirildi. PCR amplifikasyonundan sonra, PCR ürünleri agaroz jelde koşturuldu, UV cihazı ile görüntülendi ve sekanslandı. Filogenetik analiz MEGA 7'de yapılmıştır. Morfolojik ve filogenetik analizlere dayanarak CT izolatı C. truncatum olarak tanımlanmıştır. Patojenite testleri domates yaprakları ve çeri domatesleri kullanılarak gerçekleştirildi. İnokule edilen yapraklar ve domatesler, 27 °C sıcaklık ve 12:12 saat aydınlık-karanlık koşulları altındaki iklim odasında nemli bir filtre kağıdı üzerinde inkübe edilmiştir. İnokülasyonun 7. gününde enfekteli dokularda acervuli gözlenmiştir. Haplotip, haplotip sayısı ve nükleotid çeşitliliği DnaSP 6.0 yazılımı ile analiz edilmiştir. Farklı ülkelerden 46 C. truncatum izolatının ITS sekansına göre 8 haplotip belirlenmiştir. Haplotiplerin medyan birleştirme ağı analizi NETWORK 10 programı kullanılarak çizilmiştir. Türkiye'den bu çalışma ile rapor edilen CT izolatının ve domateste rapor edilen diğer referans izolatların en yaygın haplotip olan H1'de olduğu belirlenmiştir.

Kaynakça

  • Almaraz Sánchez, A., Ayala Escobar, V., Landero Valenzuela, N., Tlatilpa Santamaría, I. F., & Nieto Angel, D. (2019). First Report of Colletotrichum truncatum of Solanum lycopersicum in Mexico. Plant Disease 103 (7). doi: 10.1094/PDIS-10-18-1809-PDN
  • Anonymous (2017). https://www.seminis-us.com/resources/disease-guides/tomatoes/anthracnose-2/. Accession date: 15.02.21
  • Belov, G. L., Belosokhov, A. F., Kutuzova, I. A., Statsyuk, N. V., Chudinova, E. M., Alexandrova, A. V., Kokaeva, L. Y., & Elansky, S. N. (2018). Colletotrichum coccodes in potato and tomato leaves in Russia. Journal of Plant Diseases and Protection 125: 311–317. doi:10.1007/s41348-017-0138-0
  • Carbone, I., & Kohn, L. (2004). Inferring process from pattern in fungal population genetics. In: Khachatourians, G. G., Arora, D. K. (eds). Fungal Genomics, vol. 4. Elsevier Science B.V, Amsterdam
  • Damm, U., Woudenberg, J. H. C., Cannon, P. F., & Crous, P. W. (2009). Colletotrichum species with curved conidia from herbaceous hosts. Fungal Diversity 39: 45–87.
  • Damm, U., Cannon, P. F., Woudenberg, J. H., Johnston, P. R., Weir, B. S., Tan, Y. P., Shivas, R. G., & Crous, P. W. (2012). The Colletotrichum boninense species complex. Studies in Mycology 73 (1): 1–36. doi:10.3114/sim0002
  • da Silva, L. L., Moreno, H. L. A., Correia, H. L. N., Santana, M. F., & de Queiroz, M. V. (2020). Colletotrichum: species complexes, lifestyle, and peculiarities of some sources of genetic variability. Applied Microbiology and Biotechnology 104(5):1891-1904.
  • Diao, Y. Z., Zhang, C., Lin, D., & Liu, X. L. (2014). First report of Colletotrichum truncatum causing anthracnose of tomato in China. Plant Disease 98 (5): 687. doi: 10.1094/PDIS-05-13-0491-PDN
  • FAO (2020). Production quantities of tomatoes by country, 1994-2018. http://www.fao.org/faostat/en/#data/QC/visualize
  • He, Y., Chen, Q., Shu, C., Yang, M., & Zhou, E. (2016). Colletotrichum truncatum, a new cause of anthracnose on Chinese flowering cabbage (Brassica parachinensis) in China. Tropical Plant Pathology 41: 183–192. doi:10.1007/s40858-016-0086-4
  • Isaac, S. (1992). Fungal Plant Interaction. Chapman and Hall Press, London, 115 p. Jayawardena, R. S., Hyde, K., Damm, U., Cai, L., Liu, M., Li, X., Zhang, W., Zhao, W., & Yan, J. (2016) Notes on currently accepted species of Colletotrichum. Mycosphere 7(8):1192–1260. https://doi.org/10. 5943/mycosphere/si/2c/9
  • Katoch, A., Prabhakar, C. S., & Sharma, P. N. (2016). Metageographic population analysis of Colletotrichum truncatum associated with chili fruit rot and other hosts using ITS region nucleotide sequences. Journal of Plant Biochemistry and Biotechnology 25 (1): 64–72. doi: 10.1007/s13562-015-0310-1
  • Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111-120.
  • Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA 7: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35: 1547-1549.
  • Lefort, F., Lally, M., Thompson, D., & Douglas, G. C. (1998). Morphological traits microsatellite fingerprinting and genetic relatedness of a stand of elite oaks (Q. robur L.) at Tuallynally, Ireland. Silvae Genetica 47: 5-6.
  • Marcelino, J., Giordano, R., Gouli, S., Gouli, V., Parker, B L., Skinner, M., TeBeest, D., & Cesnik, R. (2008). Colletotrichum acutatum var. fioriniae (teleomorph: Glomerella acutata var. fioriniae var. nov. infection of a scale insect. Mycologia 100 (3): 353–374.
  • Marcelino, J. A., Gouli, S., Parker, B. L., Skinner, M. & Giordano, R. (2009). Entomopathogenic activity of a variety of the fungus, Colletotrichum acutatum, recovered from the elongate hemlock scale, Fiorinia externa. Journal of Insect Science 9: 13. doi:10.1673/031.009.1301
  • Posada, D., & Crandall, K. A. (2001). Intraspecific gene genealogies: trees grafting into networks. Trends in Ecology & Evolution 1: 37–45.
  • Ranathunge, N. P., & Sandani, H. B. P. (2016). Deceptive behaviour of Colletotrichum truncatum: strategic survival as an asymptomatic endophyte on non-host species. Journal of Plant Protection Research 56 (2): 157-162. doi:10.1515/jppr-2016-0026
  • Rao, S., Sharda, S., Oddi, V., & Nandineni, M. R. (2018). The landscape of repetitive elements in the refined genome of Chilli anthracnose fungus Colletotrichum truncatum. Frontiers in Microbiology 9, 2367. doi:10.3389/fmicb.2018.02367
  • Rogério, F., Ciampi-Guillardi, M., Barbieri, M. C., Bragança, C. A., Seixas, C. D., Almeida, A. M., & Massola, N. S. (2017). Phylogeny and variability of Colletotrichum truncatum associated with soybean anthracnose in Brazil. Journal of Applied Microbiology 122 (2): 402-415. doi: 10.1111/jam.13346. PMID: 27859958
  • Saini, T. J., Gupta, S. G., & Anandalakshmi, R. (2017). Detection of tomato anthracnose caused by Colletotrichum truncatum in India. Australasian Plant Disease Notes 12: 48. doi:10.1007/s13314-017-0271-4
  • Shivaprakash, M. R., Appannanavar, S. B., Dhaliwal, M., Gupta, A., Gupta, S., Gupta, A., & Chakrabarti, A. (2011). Colletotrichum truncatum: an unusual pathogen causing mycotic keratitis and endophthalmitis. Journal of Clinical Microbiology 49 (8): 2894–2898. doi:10.1128/JCM.00151-11
  • Torres-Calzada, C., Tapia-Tussell, R., Higuera-Ciapara, I., Huchin-Poot, E., Martin-Mex, R., Nexticapan-Garcez, A., & Perez-Brito, D. (2018). Characterization of Colletotrichum truncatum from papaya, pepper and physic nut based on phylogeny, morphology and pathogenicity. Plant Pathology 67 (4): 821-830.
  • Villafana, R. T., Ramdass, A. C., & Rampersad, S. N. (2018). First report of Colletotrichum truncatum causing anthracnose in tomato fruit in Trinidad. Plant Disease 102 (9): 1857. doi:10.1094/PDIS-02-18-0319-PDN
  • White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M. A., Gelfand, D. H., Sninsky, J. J., & White, T. J. (eds). PCR Protocols: A Guide to Methods and Applications Academic Press, New York, pp 315-322. http://dx.doi.org/10.1016/B978-0-12-372180-8.50042-1
  • Wikee, S., Cai, L., Pairin, N., McKenzie, E. H. C., Su, Y. Y., Chukeatirote, E., Thi, H. N., Bahkali, A. H., Moslem, M. A., Abdelsalam, K., & Hyde, K. D. (2011). Colletotrichum species from Jasmine (Jasminum sambac). Fungal Diversity 46, 171–182
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm Makaleler
Yazarlar

Esra Gül 0000-0002-8001-3412

Yayımlanma Tarihi 15 Eylül 2021
Kabul Tarihi 13 Ağustos 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 31 Sayı: 3

Kaynak Göster

APA Gül, E. (2021). Characterization and Haplotype Analysis of Colletotrichum truncatum in Greenhouse Tomato in Turkey. Yuzuncu Yıl University Journal of Agricultural Sciences, 31(3), 776-785. https://doi.org/10.29133/yyutbd.912293

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