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Bioprospecting of Fragrant Ginger (Zingiber aromaticum) Endophytic Bacteria from Enggano Island, Indonesia as Antimicrobial Compounds Producer

Yıl 2024, Cilt: 34 Sayı: 2, 263 - 270, 30.06.2024
https://doi.org/10.29133/yyutbd.1429698

Öz

Fragrant Ginger or Lempuyang wangi (Zingiber aromaticum Val.) is one of the plants from the Zingiberaceae family that Indonesians widely use as traditional medicine. Endophytic bacteria living in the healthy plant are potentially carrying antimicrobial properties and good secondary metabolites. This study aims to determine the potential of endophytic bacteria from fragrant ginger plants from Enggano Island as antimicrobial. Antimicrobial activity was analyzed using the disc diffusion method from pallets and supernatant of bacteria. The results showed that five of 44 isolates consisting of Providencia strain LWERG 29, Stenotrophomonas strain LWERG 30, Bacillus strain LWEBG 39, Bacillus strain LWEBG 41, and Pseudomonas strain LWEBG 42 isolates were able to suppress pathogenic bacteria such as B. subtilis, P. aeruginosa, and E. coli. Interestingly, those selected species could show their ability to inhibit tested pathogens with a strong category. This is the first study that showed the potential of endophytic bacteria as antimicrobial agents isolated from fragrant ginger (Lempuyang Wangi) in Enggano Island, Indonesia.

Kaynakça

  • Abednego, R. N., & Silago, V. (2023). Antibacterial activity of soil-isolated Bacillus altitudinis/pumilus complex against methicillin-resistant Staphylococcus aureus from Mwanza, Tanzania. African Journal of Laboratory Medicine, 12, a2167.
  • Aji, O. R., & Zakkiyah, H. C. (2021). Antifungal Activity of 96% Ethanol Extract of Lempuyang Wangi Rhizome (Zingiber aromaticum Val.) against Pythium sp. Fungi in Vitro. Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 6(1), 58–63.
  • Andeas, A. F., Wibowo, R. H., Darwis, W., Sipriyadi, Supriati, R., Hidayah, T., & Supriyanto, A. P. (2023). Isolation and identification of endophytic bacteria in lempuyang wangi (Zingiber zerumbeth var. aromaticum Val.) from Enggano Island, Bengkulu Province. AIP Conference Proceedings, 2606(1), 050007.
  • Anggraini, L., Marlida, Y., Wizna, W., Jamsari, J., Mirzah, M., Adzitey, F., & Huda, N. (2018). Molecular identification and phylogenetic analysis of GABA-producing lactic acid bacteria isolated from indigenous dadih of West Sumatera, Indonesia. F1000Research, 7, 1663.
  • Cappucino, J., & Welsh, C. (2018). Microbiology: a laboratory manual (17th ed.). Pearson Education Limited.
  • Dahlan, M. S. (2014). Statistics for Medicine and Health. Jakarta: Epidemiology Indonesia. Indonesia Health Profile Data and Information, 6, 7-33.
  • Duhan, P., Bansal, P., & Rani, S. (2020). Isolation, identification and characterization of endophytic bacteria from medicinal plant Tinospora cordifolia. South African Journal of Botany, 134, 43-49.
  • Fani, E., Rahmawati, R., & Kurniatuhadi, R. (2022). Identification And Detection Of Proteolytic Activity Of Endophite Bacterial Isolated From Avicennia Marina Leaves In Mempawah Mangrove Center. LenteraBio: Berkala Ilmiah Biologi, 11(2), 293–299.
  • Ghequire, M. G. K., & Öztürk, B. (2018). A Colicin M-Type Bacteriocin from Pseudomonas aeruginosa Targeting the HxuC Heme Receptor Requires a Novel Immunity Partner. Applied and Environmental Microbiology, 84(18), e00716-18.
  • Hamidy, A., Keim, A., Sihotang, V., Lupiyaningdyah, P., Irham, M., Ardiyani, M., Kertonegoro, A., Wiantoro, S., Khusniati, T., Susanti, R., Wardani, W., & Royyani, M. (2017). Ekspedisi Pulau Enggano. In I. Maryanto, A. Hamidy, A. Keim, V. Sihotang, P. Lupiyaningdyah, M. Irham, & M. Ardiyani (Eds.), LIPI Press.
  • Hanafy, A. M., Alreedy, R. M., Elbahloul, Y., & El-Sayed, W. S. (2023). Diversity of Bacteriocin-encoding Gene Families and the Activity Spectrum among Bacillus amyloliquefaciens Isolates. Egyptian Journal of Botany, 63, 69-83.
  • Hossain, C. M., Ryan, L. K., Gera, M., Choudhuri, S., Lyle, N., Ali, K. A., & Diamond, G. (2022). Antifungals and Drug Resistance. Encyclopedia, 2, 1722-1737.
  • Leong-Skornickova, J., Souvannakhoummane, K., & Tran, H. (2019). Zingiber zerumbet (Shampoo Ginger). The IUCN Red List of Threatened Species 2019: E.T96816015A96816082.
  • Mamangkey, J., Mendes, L. W., Sibero, M. T., & Nadapdap, P. E. (2020). Bioprospecting For Bacterial Endophytes Associated With Zingiberaceae Family Rhizomes In Sibolangit Forest, North Sumatera. International Journal of Science, Technology & Management, 1(1), 27-36.
  • Nurjannah, L., Azhari, A., & Supratman, U. (2023). Secondary Metabolites of Endophytes Associated with the Zingiberaceae Family and Their Pharmacological Activities. Scientia Pharmaceutica, 91, 3.
  • Ouchari, L., Boukeskasse, A., Bouizgarne, & Ouhdouch, Y. (2019). Antimicrobial Potential Of Actinomycetes Isolated From The Unexplored Hot Merzouga Desert and Their Taxonomic Diversity. Biology Open, 8(2), 1-7.
  • Raio, A., Brilli, F., Neri, L., Baraldi, R., Orlando, F., Pugliesi, C., Chen, X., & Baccelli, I. (2023). Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes. Frontiers in plant science, 14, 1235669.
  • Reygaert, W. C. (2018). An overview of the antimicrobial resistance mechanisms of bacteria. AIMS Microbiology, 4(3), 482–501.
  • Rori, C. A., Kandou, F. E. F., & Agustina, M. T. (2020). Extracellular Enzyme Activity of Mangrove Plant Endophytic Bacteria Avicennia marina. Jurnal Bios Logos, 10(2), 48–55.
  • Uçar, C. P., Selem, E., Tunçtürk, R., Tunçtürk, M., & Akköprü, A. (2023). The effect of some endophytic bacteria on seedling growth and physiological properties of Salvia officinalis L. Yuzuncu Yil University Journal of Agricultural Sciences, 33(1), 130-139.
  • Wibowo, R. H., Sipriyadi, Darwis, W., Putri, D. A., Yudha, S., Mashudi, Ilsan, N. A., Renta, P. P., & Masrukhin. (2023). Isolation, characterization and identification of sponge-associated bacteria producing antimicrobial compounds. Biodiversitas, 24, 3616-3623.
  • Xie, Y., Peng, Q., Ji, Y., Xie, A., Yang, L., Mu, S., Li, Z., He, T., Xiao, Y., Zhao, J., & Zhang, Q. (2021). Isolation and identification of antibacterial bioactive compounds from Bacillus megaterium L2. Frontiers in Microbiology, 12, 645484.
Yıl 2024, Cilt: 34 Sayı: 2, 263 - 270, 30.06.2024
https://doi.org/10.29133/yyutbd.1429698

Öz

Kaynakça

  • Abednego, R. N., & Silago, V. (2023). Antibacterial activity of soil-isolated Bacillus altitudinis/pumilus complex against methicillin-resistant Staphylococcus aureus from Mwanza, Tanzania. African Journal of Laboratory Medicine, 12, a2167.
  • Aji, O. R., & Zakkiyah, H. C. (2021). Antifungal Activity of 96% Ethanol Extract of Lempuyang Wangi Rhizome (Zingiber aromaticum Val.) against Pythium sp. Fungi in Vitro. Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 6(1), 58–63.
  • Andeas, A. F., Wibowo, R. H., Darwis, W., Sipriyadi, Supriati, R., Hidayah, T., & Supriyanto, A. P. (2023). Isolation and identification of endophytic bacteria in lempuyang wangi (Zingiber zerumbeth var. aromaticum Val.) from Enggano Island, Bengkulu Province. AIP Conference Proceedings, 2606(1), 050007.
  • Anggraini, L., Marlida, Y., Wizna, W., Jamsari, J., Mirzah, M., Adzitey, F., & Huda, N. (2018). Molecular identification and phylogenetic analysis of GABA-producing lactic acid bacteria isolated from indigenous dadih of West Sumatera, Indonesia. F1000Research, 7, 1663.
  • Cappucino, J., & Welsh, C. (2018). Microbiology: a laboratory manual (17th ed.). Pearson Education Limited.
  • Dahlan, M. S. (2014). Statistics for Medicine and Health. Jakarta: Epidemiology Indonesia. Indonesia Health Profile Data and Information, 6, 7-33.
  • Duhan, P., Bansal, P., & Rani, S. (2020). Isolation, identification and characterization of endophytic bacteria from medicinal plant Tinospora cordifolia. South African Journal of Botany, 134, 43-49.
  • Fani, E., Rahmawati, R., & Kurniatuhadi, R. (2022). Identification And Detection Of Proteolytic Activity Of Endophite Bacterial Isolated From Avicennia Marina Leaves In Mempawah Mangrove Center. LenteraBio: Berkala Ilmiah Biologi, 11(2), 293–299.
  • Ghequire, M. G. K., & Öztürk, B. (2018). A Colicin M-Type Bacteriocin from Pseudomonas aeruginosa Targeting the HxuC Heme Receptor Requires a Novel Immunity Partner. Applied and Environmental Microbiology, 84(18), e00716-18.
  • Hamidy, A., Keim, A., Sihotang, V., Lupiyaningdyah, P., Irham, M., Ardiyani, M., Kertonegoro, A., Wiantoro, S., Khusniati, T., Susanti, R., Wardani, W., & Royyani, M. (2017). Ekspedisi Pulau Enggano. In I. Maryanto, A. Hamidy, A. Keim, V. Sihotang, P. Lupiyaningdyah, M. Irham, & M. Ardiyani (Eds.), LIPI Press.
  • Hanafy, A. M., Alreedy, R. M., Elbahloul, Y., & El-Sayed, W. S. (2023). Diversity of Bacteriocin-encoding Gene Families and the Activity Spectrum among Bacillus amyloliquefaciens Isolates. Egyptian Journal of Botany, 63, 69-83.
  • Hossain, C. M., Ryan, L. K., Gera, M., Choudhuri, S., Lyle, N., Ali, K. A., & Diamond, G. (2022). Antifungals and Drug Resistance. Encyclopedia, 2, 1722-1737.
  • Leong-Skornickova, J., Souvannakhoummane, K., & Tran, H. (2019). Zingiber zerumbet (Shampoo Ginger). The IUCN Red List of Threatened Species 2019: E.T96816015A96816082.
  • Mamangkey, J., Mendes, L. W., Sibero, M. T., & Nadapdap, P. E. (2020). Bioprospecting For Bacterial Endophytes Associated With Zingiberaceae Family Rhizomes In Sibolangit Forest, North Sumatera. International Journal of Science, Technology & Management, 1(1), 27-36.
  • Nurjannah, L., Azhari, A., & Supratman, U. (2023). Secondary Metabolites of Endophytes Associated with the Zingiberaceae Family and Their Pharmacological Activities. Scientia Pharmaceutica, 91, 3.
  • Ouchari, L., Boukeskasse, A., Bouizgarne, & Ouhdouch, Y. (2019). Antimicrobial Potential Of Actinomycetes Isolated From The Unexplored Hot Merzouga Desert and Their Taxonomic Diversity. Biology Open, 8(2), 1-7.
  • Raio, A., Brilli, F., Neri, L., Baraldi, R., Orlando, F., Pugliesi, C., Chen, X., & Baccelli, I. (2023). Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes. Frontiers in plant science, 14, 1235669.
  • Reygaert, W. C. (2018). An overview of the antimicrobial resistance mechanisms of bacteria. AIMS Microbiology, 4(3), 482–501.
  • Rori, C. A., Kandou, F. E. F., & Agustina, M. T. (2020). Extracellular Enzyme Activity of Mangrove Plant Endophytic Bacteria Avicennia marina. Jurnal Bios Logos, 10(2), 48–55.
  • Uçar, C. P., Selem, E., Tunçtürk, R., Tunçtürk, M., & Akköprü, A. (2023). The effect of some endophytic bacteria on seedling growth and physiological properties of Salvia officinalis L. Yuzuncu Yil University Journal of Agricultural Sciences, 33(1), 130-139.
  • Wibowo, R. H., Sipriyadi, Darwis, W., Putri, D. A., Yudha, S., Mashudi, Ilsan, N. A., Renta, P. P., & Masrukhin. (2023). Isolation, characterization and identification of sponge-associated bacteria producing antimicrobial compounds. Biodiversitas, 24, 3616-3623.
  • Xie, Y., Peng, Q., Ji, Y., Xie, A., Yang, L., Mu, S., Li, Z., He, T., Xiao, Y., Zhao, J., & Zhang, Q. (2021). Isolation and identification of antibacterial bioactive compounds from Bacillus megaterium L2. Frontiers in Microbiology, 12, 645484.
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Tarımda Bitki Bakteriolojisi
Bölüm Makaleler
Yazarlar

Risky Hadı Wıbowo 0000-0002-5917-4625

Sipriyadi Sipriyadi 0000-0003-1042-2576

Welly Darwıs 0000-0002-5982-8983

Eddy Sukmawinata 0000-0002-8749-0270

Masrukhin Masrukhin 0000-0001-8853-6034

Mashudi Mashudi 0009-0001-4819-505X

Muhammad Asrıl 0000-0001-8637-4190

Thoriqul Hıdayah 0009-0005-2719-3046

Aldy Trıanda 0009-0004-2608-7527

Erken Görünüm Tarihi 16 Haziran 2024
Yayımlanma Tarihi 30 Haziran 2024
Gönderilme Tarihi 2 Şubat 2024
Kabul Tarihi 22 Mart 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 34 Sayı: 2

Kaynak Göster

APA Hadı Wıbowo, R., Sipriyadi, S., Darwıs, W., Sukmawinata, E., vd. (2024). Bioprospecting of Fragrant Ginger (Zingiber aromaticum) Endophytic Bacteria from Enggano Island, Indonesia as Antimicrobial Compounds Producer. Yuzuncu Yıl University Journal of Agricultural Sciences, 34(2), 263-270. https://doi.org/10.29133/yyutbd.1429698

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