Year 2015, Volume 16, Issue 1, Pages 9 - 15 2016-03-16

MARMARA BÖLGESİNDE BAZI KÜLTÜR BİTKİLERİNDE DOĞAL ENFEKSİYONA NEDEN OLAN CUCUMBER MOSAİC VİRUS (CMV)’UNUN ARAŞTIRILMASI
Detection of Cucumber Mosaic Virus (Cucumber Mosaic Virus, Cmv) Causing Natural Infection on Some Cultured Plants in Marmara Region

Nesrin UZUNOĞULLARI [1] , Mustafa Gümüş [2]

901 1169

Bu çalışmada 2013 yılı Haziran ve Temmuz aylarında Çanakkale (Ezine)’den 48 domates, Bursa (İznik, Orhangazi, Yenişehir)’dan 15 domates, 25 biber, 35 glayöl, Yalova (Merkez)’dan 12 hıyar, 8 kabak, 16 biber, İstanbul (Pendik)’dan 25 biber, Bilecik (Osmaneli)’den 14 domates, 15 biber ve Sakarya (Pamukova)’dan 22 biber olmak üzere toplam 235 adet bitki yaprak örneği toplanmıştır. Örnekler DAS-ELISA yöntemiyle analiz edilmiştir. Toplanan biber örneklerinin % 69’u, domates örneklerinin % 87’si, kabak örneklerinin % 75’i, salatalık örneklerinin % 58’i ve glayöl örneklerinin % 69’u Cucumber mosaic virus (CMV) ile enfekteli bulunmuştur. Real-Time PCR analizinde glayöl, biber, domates, kabak ve hıyar örnekleri 84.0-88.7 değerleri arasında pik verirken negatif kontrol 76.0 değerinde pik vermiştir. PCR analizi sonucunda 540 bp’lik DNA bantları % 1’ lik agaroz jel elektroforezde gözlenmiştir. Moleküler ve serolojik analizler paralellik göstermiştir. Bu çalışma, CMV’nin glayöl bitkisinde tespitinin Türkiye’deki ilk kaydıdır.

In this study, a total of 235 plant leaf samples were collected from 48 tomatoes from Çanakkale (Ezine), 15 tomatoes, 25 peppers and 35 gladiolus from Bursa (İznik, Yenişehir, Orhangazi), 12 cucumbers, 8 zucchini and 16 peppers from Yalova (Center), 25 peppers from Istanbul (Pendik), 14 tomatoes and 15 peppers from Bilecik (Osmaneli) and from 22 peppers from Sakarya (Pamukova) in June and July 2013. Samples were analysed by DAS-ELISA technique. 69 % of pepper samples, 87 % of tomato samples, 75 % of zucchini samples, 58 % of cucumbers sample and 69 % of gladiolus samples were found to be infected with Cucumber mosaic virus (CMV). Real-Time PCR analysis in the gladiolus, peppers, tomatoes, zucchini and cucumber samples revealed peak values between 84.0-88.7, while the negative control gave a peak value of 76.0. The expected amplicon sizes of 540 bp were observed after electrophoresis of PCR products in 1% agarose gel. The results of ELISA and PCR showed parallelism. This study is the first report of CMV in gladiolus in Turkey. 

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Subjects Biology
Journal Section Research Article/Araştırma Makalesi
Authors

Author: Nesrin UZUNOĞULLARI

Author: Mustafa Gümüş

Bibtex @ { trkjnat267858, journal = {Trakya University Journal of Natural Sciences}, issn = {2147-0294}, eissn = {2528-9691}, address = {Trakya University}, year = {2016}, volume = {16}, pages = {9 - 15}, doi = {}, title = {Detection of Cucumber Mosaic Virus (Cucumber Mosaic Virus, Cmv) Causing Natural Infection on Some Cultured Plants in Marmara Region}, key = {cite}, author = {UZUNOĞULLARI, Nesrin and Gümüş, Mustafa} }
APA UZUNOĞULLARI, N , Gümüş, M . (2016). Detection of Cucumber Mosaic Virus (Cucumber Mosaic Virus, Cmv) Causing Natural Infection on Some Cultured Plants in Marmara Region. Trakya University Journal of Natural Sciences, 16 (1), 9-15. Retrieved from http://dergipark.org.tr/trkjnat/issue/25380/267858
MLA UZUNOĞULLARI, N , Gümüş, M . "Detection of Cucumber Mosaic Virus (Cucumber Mosaic Virus, Cmv) Causing Natural Infection on Some Cultured Plants in Marmara Region". Trakya University Journal of Natural Sciences 16 (2016): 9-15 <http://dergipark.org.tr/trkjnat/issue/25380/267858>
Chicago UZUNOĞULLARI, N , Gümüş, M . "Detection of Cucumber Mosaic Virus (Cucumber Mosaic Virus, Cmv) Causing Natural Infection on Some Cultured Plants in Marmara Region". Trakya University Journal of Natural Sciences 16 (2016): 9-15
RIS TY - JOUR T1 - Detection of Cucumber Mosaic Virus (Cucumber Mosaic Virus, Cmv) Causing Natural Infection on Some Cultured Plants in Marmara Region AU - Nesrin UZUNOĞULLARI , Mustafa Gümüş Y1 - 2016 PY - 2016 N1 - DO - T2 - Trakya University Journal of Natural Sciences JF - Journal JO - JOR SP - 9 EP - 15 VL - 16 IS - 1 SN - 2147-0294-2528-9691 M3 - UR - Y2 - 2019 ER -
EndNote %0 Trakya University Journal of Natural Sciences Detection of Cucumber Mosaic Virus (Cucumber Mosaic Virus, Cmv) Causing Natural Infection on Some Cultured Plants in Marmara Region %A Nesrin UZUNOĞULLARI , Mustafa Gümüş %T Detection of Cucumber Mosaic Virus (Cucumber Mosaic Virus, Cmv) Causing Natural Infection on Some Cultured Plants in Marmara Region %D 2016 %J Trakya University Journal of Natural Sciences %P 2147-0294-2528-9691 %V 16 %N 1 %R %U
ISNAD UZUNOĞULLARI, Nesrin , Gümüş, Mustafa . "Detection of Cucumber Mosaic Virus (Cucumber Mosaic Virus, Cmv) Causing Natural Infection on Some Cultured Plants in Marmara Region". Trakya University Journal of Natural Sciences 16 / 1 (March 2016): 9-15.