A Study on the Presence of Potato Cyst Nematodes: Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 and Globodera pallida Stone 1973 (Tylenchida: Heteroderidae) in Nevşehir Province, Turkey

Year 2019, Volume: 6 Issue: 2, 125 - 129, 22.04.2019

Adem Özarslandan , Dilek Dınçer Refik Bozbuga Mustafa İmren İbrahim Halil Elekcıoğlu

https://doi.org/10.30910/turkjans.556586

Cited By: 2

Abstract

Potato (Solanum tuberosum L.) cyst nematodes (Globodera rostochiensis (Wollenweber)
and Globodera pallida (Stone) cause
significant yield losses and have been listed as quarantine pests for many
countries in the world including Turkey, and the European and Mediterranean
Plant Protection Organization (EPPO, A2). In Turkey, the current potato
production area is decreasing due to contamination by quarantine agents, and
new fields are under threat of invasion by new quarantine organisms. In this
study, samples were collected from Nevşehir province, Turkey, in potato growing
fields during the spring growing season in 2014-2015. Soil samples were washed
using Fenwick’s can and cysts were extracted and identified using molecular
markers. The species-specific molecular markers generated a 435 bp using
PITSr3, PITSp4 and ITS5 primers. The potato golden cyst nematode, G. rostochiensis was identified in
Nevşehir potato production areas and this is the first report for the Central
Anatolia Region. In addition, G.
rostochiensis was found in two samples of the same field in the surveys. The
average cyst nematode density was determined as 60 cysts in 250 g soil.

Keywords

Potato, cyst nematode, molecular identification, density

Nevşehir İlinde Patates Kist Nematodlarının Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 ve Globodera pallida Stone 1973 (Tylenchida: Heteroderidae) Belirlenmesi

Abstract

Patates
(Solanum tuberosum L.) kist
nematodları (Globodera rostochiensis
(Wollenweber) ve Globodera pallida
(Stone) önemli verim kayıplarına neden olmakta, Türkiye de dahil olmak üzere
dünyadaki birçok ülke, Avrupa ve Akdeniz Bitki Koruma Örgütü’nde (EPPO, A2)
karantina zararlıları olarak listelenmiştir. Türkiye'de mevcut patates üretim
alanı karantina zararlısı olarak bulaşmaya bağlı olarak azalmakta ve yeni
alanlar yeni karantina organizmaları tarafından istila tehdidi altında
bulunmaktadır. Bu çalışmada, Türkiye, Nevşehir ilinden 2014-2015 yıllarında
bahar mevsiminde patates yetiştirme alanlarında örnekler toplanmıştır. Toprak örnekleri Fenwick aletinde yıkanarak
bulaşık örneklerdeki kistler elde edilmiş ve kist popülasyonlarının moleküler olarak teşhisleri yapılmıştır. Kist popülasyonlarının
moleküler düzeyde türe özgü PITSr3,
PITSp4 ve ITS5 primerleri kullanılarak yapılan teşhiste söz konusu primerlerin
435 bp de bant oluşturduğu tespit edilmiştir. Bu çalışma sonucunda Nevşehir ili patates ekiliş alanlarında patates kist
nematodu G. rostochiensis saptanmış
olup, bu sonuç İç Anadolu Bölgesi için ilk kayıt olma özelliğini taşımaktadır.
Ayrıca, sürveylerde aynı tarlaya ait iki örnekte G. rostochiensis’e rastlanılmış,
250 g toprakta ortalama 60 kist yoğunluğunda olduğu tespit edilmiştir.

Keywords

Patates, kist nematodu, moleküler teşhis, yoğunluk

References

• Bates, J.A., Taylor, E.J., Gans, P.T., Thomas, J.E. 2002. Determination of relative proportions of Globodera species in mixed populations of potato cyst nematodes using PCR product melting peak analysis. Molecular Plant Pathology, 3: 153-161.
• Brown, E.B. 1969. Assessment of the damage caused to potatoes by potato cyst eelworm, Heterodera rostochiensis Woll. Annals of Applied Biology, 63: 493-502. Bulman, S.R., Marshall, J.W. 1997. Differentiation of Australian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR). New Zealand Journal of Crop and Horticultural Science, 25: 123-129.
• Erdogan, P., Kilic T., Ulutas E., Kepenekci, I., Toktay, H., Cakir, E., Aydin, M.H., Karahan, A., Ustun, N., Ozdemir, S., Akbas, B., Yurtmen, M., Basaran, S., Elibuyuk, E.A., Velioglu, A.S., Erdogan, C., Kodan, M., Sabahoglu, Y., Aydar, A., Akbas, R., Hantas, C. 2011. Editors: Atlamaz A., Gokce A.Y. Patates Entegre Mücadele Teknik Talimatı. Gıda Tarım ve Hayvancılık Bakanlığı, Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü, Bitki Sağlığı Araştırmaları Daire Başkanlığı. Ankara, p. 119.
• EPPO, 2004. Diagnostic protocols for regulated pests. Globodera rostochiensis and Globodera pallida. EPPO Bulletin, 34: 309-314.
• Evans, K., Trudgill. D.L. 1992. Pest aspects of potato production Part 1. The nematode pests of potatoes. Ed: P.Harris. "Potato Crop" Chapman, Hill London, pp. 438-475. FAO, 2013. Food and Agricultural Organization of United Nations. http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567#ancor.
• Fenwick, D.W. 1940. Methods for the recovery and counting of cysts of Heterodera schachtii from soil. Journal of Helminthology, 18: 155-172.
• Fullaondo, A., Barrena, E., Viribay, M., Barrena, I., Salazar, A., Ritter, E. 1999. Identification of potato cyst nematode species Globodera rostochiensis and G. pallida by PCR using specific primer combinations. Nematology, 2: 157-163.
• Holgado, R., Magnusson, C. 2012. Nematodes as a Limiting Factor in Potato Production in Scandinavia. Potato Research, 55: 269-278.
• Jatala, P. 1978. Parasitic Nematodes of potatoes CIP technical Information Bulletin. 8: 17.
• Jensen, H.J. 1978. Nematode pests of vegetable and related crops Ed:Webster "Economic nematology" Academic Press., London and New York, pp. 377-408.
• Madani, M., Ward, L.J., De Boer, S.H. 2008. Multiplex real-time polymerase chain reaction for identifying potato cyst nematodes, Globodera pallida and Globodera rostochiensis, and the tobacco cyst nematode, Globodera tabacum. Canadian Journal of Plant Pathology, 30: 554-564.
• Mendoza, H.A., Jatala, P. 1978. Breeding for resistance to the root-knot nematode. International Potato, Center Report of the planning conference on the developments in the control of nematode pest of potatoes II. International Potato center, Lima, Peru, pp. 111-116.
• Ozarslandan, A., Devran, Z., Mutlu, N., Elekcioglu, I.H. 2009. First Report of Columbia Root-Knot Nematode (Meloidogyne chitwoodi) in Potato in Turkey. Plant Disease, 93: 316.
• Phillips, M.S., Hackett, C.A. and Trudgill, D.L. 1991. The relationship between the initial and final population densities of the potato cyst nematode Globodera pallida for partially resistant potatoes. Journal of Applied Ecology, 28: 109-119.
• Powers, T. 2004. Nematode molecular diagnostics: from bands to barcodes. Annual Review of Phytopathology, 42: 367-383.
• CABI, 2013. Globodera pallida. Crop Protection Compendium. Accessed September 23, 2013 from: www.cabi.org/cpc
• Pylypenko, L.A., Uehara, T., Phillips, M.S., Sigareva, D.D., Blok, V.C. 2005. Identification of Globodera rostochiensis and G. pallida in the Ukraine by PCR. European Journal of Plant Pathology, 111: 39-46.
• Skantar, A.M., Handoo, Z.A., Carta, L.K., Chitwood, D.J. 2007. Morphological and molecular identification of Globodera pallida associated with potato in Idaho. Journal of Nematology, 39: 133-144.
• Tovar, A., Cid Del Prado, I., Sandoval, J., Martínez, A., Nicol, J., Evans, K. 2006. Los nematodos formadores de quistes en México. Revista Mexicana de Fitopatología, 24:145-151.
• TUIK, 2012. T.C. Başbakanlık Türkiye İstatistik Kurmu, Ankara (www.tuik.gov.tr, Access date: June, 2014).
• Turner, S.J., Evans, K. 1998. The origins, global distribution and biology of potato cyst nematodes (Globodera rostochiensis (Woll.) and Globodera pallida Stone). pp. 7-26. In: R.J. Marks and B.B. Brodie (Eds.). Potato Cyst Nematodes. Biology, distribution and control. CAB International, Wallingford, UK.
• Ulutas E, Özarslandan, A., Kaşkavalcı, G., Elekcioğlu, İ.H. 2012. Molecular diagnosis of Globodera rostochiensis Wollenweber (Tylenchida: Heteroderidae) in the potato growing areas of Aegean Region, Turkey. Turkish Journal of Entomology, 36(1): 155-160.
• Vejl, P., Skupinová, S., Sedlak, P., Domkarova, J. 2002. Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region´s polymorphism. Rostlinna výroba 48: 486-489.
• Waeyenberge, L., Ryss, A., Moens, M., Pinochet, J., Vrain. T.C. 2000. Molecular characterization of 18 Pratylenchus species using rDNA restriction fragment length polymorphism. Nematology, 2: 135-142.
• White, T. J., Bruns, T., Lee, S., Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics In: M.A. Innis, D.H. Gelfand, J.J. Sninsky, and T.J. White (Eds.). PCR Protocols: A guide to methods and applications. Academic Press, San Diego, California, USA, pp. 315-322.
• Winslow, R.D., Willis, R.J. 1972. Nematode Diseases of Potatoes. Economic Nematology J.M. Webster, Academic Press London, pp: 17-48.
• Zawislak, K., Niewiadomski, W., Gronowicz, H. 1981. Continuous cultivation of potatoes and the golden nematode problem. Zeszyty Naukowe Akademii Rolniczo-Technicznej Olsztyn, Rolnictwo, 29: 259-270.