Increased fat graft survival with mesenchymal stem cell recruiting effect of PRP: in vitro and in vivo study of application techniques

Year 2022, Volume: 32 Issue: 2, 207 - 214, 29.04.2022

Gokce Yildiran Osman Akdağ Gulsemin Cicek Zeliha Esin Celik Hasibe Artaç Hulya Ucaryilmaz Selcuk Duman Mustafa Sutcu Tahsin Murad Aktan Zekeriya Tosun

https://doi.org/10.54005/geneltip.1074859

Abstract

Aim: Adding platelet-rich plasm (PRP) or mesenchymal stem cells are the most accepted methods to increase fat graft’s permanence. However, there is no consensus on timing and whether the effect of stem cells or PRP is observed more in the recipient area or in the donor area.
It is aimed to present the application method and localization of PRP to be combined with fat graft to increase the survival.
Material and Methods: in vitro part: Fat grafts were kept in a medium containing PRP. Cell output from the fat graft to the Petri dishes was examined every day. The time to recruit the maximum number of stem cells to the medium was determined (day 10). in vivo part: Eight group of rats were received PRP in different concentrations either to recipient or donor areas of fat grafts. After ten days, fat grafts were transferred to the recipient area. Ninety days after the transfer, histopathological examination and immunohistochemical staining were performed.
Results: The group which had received the full-dose PRP to the recipient area showed the highest cellular integrity and vascularity (p< 0.05). Vascularity was superior in the half-dose PRP group compared to the control group (p< 0.05); cellular integrity did not increase. Donor site groups did not show increase in cellular integrity.
Conclusion: Although PRP starts to increase fat graft permanence by increasing vascularity, it acts by preserving the cellular integrity of the fat cells as the concentrations increase. The PRP injection to the recipient area 10 days before fat grafting may provide higher survival rates.

Keywords

fat graft, PRP, stem cell, survival

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştırma Projeleri

Project Number

17102016 ve 19401147

Thanks

This study was produced from the first author's thesis. Thank you to the staff at the SUDAM animal laboratory and SU scientific research project unit for the support.

PRP’nin mezenkimal kök hücre çağırıcı etkisi ile yağ grefti sağkalımında artış: Uygulama tekniklerinin in vitro ve in vivo çalışması

Abstract

Amaç: Yağ greftinin kalıcılığını artırmak için trombositten zengin plazma (PRP) veya mezenkimal kök hücre eklenmesi en çok kabul gören yöntemlerdir. Ancak zamanlama ve bölge açısından kök hücrenin mi yoksa PRPnin mi etkisinin daha çok gözlendiği konusunda bir fikir birliği yoktur.
Sağkalımı artırmak için yağ grefti ile kombine edilecek PRP'nin uygulama yöntemi ve lokalizasyonunun sunulması amaçlanmaktadır.
Gereç ve Yöntem: in vitro çalışma: Yağ greftleri PRP içeren besiyerinde tutulmuştur. Yağ greftinden Petri kaplarına hücre çıkışı her gün incelendi. Ortama en fazla sayıda kök hücre alma zamanı belirlendi (10. gün).
in vivo çalışma: Sekiz grup sıçana, yağ greftlerinin alıcı veya verici alanlarına farklı konsantrasyonlarda PRP uygulandı. On gün sonra alıcı bölgeye yağ greftleri transfer edildi. Transferden doksan gün sonra histopatolojik inceleme ve immünohistokimyasal boyama yapıldı.
Bulgular: Alıcı alana tam doz PRP uygulanan grup en yüksek hücresel bütünlük ve damarlanma gösterdi (p< 0.05). Yarım doz PRP grubunda vaskülarite kontrol grubuna göre daha üstündü (p< 0.05); hücresel bütünlük artmadı. Alıcı alan grupları, hücresel bütünlükte artış göstermedi.
Sonuç: PRP damarlanmayı artırarak yağ grefti kalıcılığını artırmaya başlasa da konsantras-yonlar arttıkça yağ hücrelerinin hücresel bütünlüğünü koruyarak etki eder. Yağ greftlemesinden 10 gün önce alıcı bölgeye PRP enjeksiyonu daha yüksek hayatta kalma oranları sağlayabilir.

Keywords

kök hücre, PRP, sağkalım, yağ grefti

Project Number

17102016 ve 19401147

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