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Endemik Anadolu Sığla Ağacı (Liquidambar orientalis Mill. ) Bitki Ekstraktlarının Bazı Bitki Patojeni Funguslara Karşı Antifungal Etkilerinin Belirlenmesi

Year 2018, Volume: 8 Issue: 2, 202 - 208, 31.07.2018
https://doi.org/10.17714/gumusfenbil.318796

Abstract

Geçmişten
günümüze kadar, bitkilerden elde edilen doğal antifungal maddeler alternatif
mücadele yöntemi olarak kullanılmaktadır. Bu çalışmada, ülkemizde endemik
olarak yetişen
Liquidambar orientalis Mill. bitkisinden elde edilen reçine ve yaprak methanol ekstraktının
antifungal etkileri araştırılmıştır. Çalışmamızda, hıyar ve elma bitkilerinde
sorun olan fungal hastalık etmenleri  
Fusarium
oxysporum f.sp. cucumerinum (FOC) ve Monilinia
fructigena’ya  karşı agar petri metodu kullanılarak farklı
bitki ekstraktlarının farklı konsantrasyonlardaki antifungal etkinlikleri
araştırılmıştır.  Çalışmalarda  bitki ekstraktlarının 0 (negatif kontrol), 5,
10 ve 20 mg/ml konsantrasyonları kullanılmış ve elde edilen veriler sonucunda,
miselyum gelişim engellemesi (MGE) ve Letal doz (LD50-90) değerleri
belirlenmiştir.  Ekstraktların fungal
etmenlere  karşı kullanılan her
konsantrasyonunda antifungal aktivite göstermiştir.  Konsantrasyon miktarı artıkça MGE değerlerinin
(%) arttığı belirlenmiştir.  
L.
orientalis reçine ekstraktı için en
yüksek MGE oranı,
M. fructigena’da
%100,
FOC’da ise %74 olarak
hesaplanmıştır. Bu değerlerin
, L. orientalis yaprak ekstraktı uygulamasında ise M. fructigena’da %66 ve FOC’da %51 olarak belirlenmiştir.  LD50
değerleri reçine ekstraktında
M. fructigena için 7.32 mg/ml ve FOC için 4.42  mg/ml, yaprak ekstraktında da M.
fructigena için 16.98 mg/ml  ve FOC
için 7.65mg/ml olarak belirlenmiştir.
LD90
değerleri ise reçine ekstraktında
M. fructigena için
7.68 mg/ml ve
FOC için 51.48 mg/ml
arasında, yaprak ekstraktında ise
M.
fructigena için
121.91 mg/ml ve FOC için 364.59 mg/ml olarak belirlenmiştir. Bu sonuçlara göre,
test organizmalarına karşı reçine ekstraktı, yaprak ekstraktından daha etkili
bulunmuştur. 

References

  • Ahn I.P, Chung H.S., ve Lee Y.H., 1997. Vegetative compat-ibility groups and pathogenicity among isolates of Fusarium oxysporum f. sp. cucumerinum. Plant Disease. 82, 244–246.
  • Aslan, İ. Çalmaşur, Ö., Şahin, F. ve Çağlar, Ö., 2005. Insecticidal effects of essential plant oils against Ephestia kuehniella (Zell.), Lasioderma serricorne (F.) and Sitophilus granarius (L.). Journal of Plant Diseases and Protection, 112, 257-267.
  • Basim, H., Yeğen, O. ve Zeller, W., 2000. Antibacterial effect of essential oil of T. Spicata L. var. spicata on some plant pathogenic bacteria. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, 107, 279-284.
  • Basim, E. ve Basim, H., 2013. Antibacterial activity of Turkish endemic sığla (Liquidambar orientalis Mill. var. orientalis) storax against agricultural plant pathogenic bacteria and its use as a seed protectant. Journal of Food, Agriculture & Environment, 11(3&4), 2447-2450.
  • Baydar, H., Sağdıç, O., Ozkan, G. ve Karadoğan, T., 2004. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control, 15, 169-172.
  • Benjilali, B., Tantadui-Elaraki, A., Ayadi, A. ve Ihlal, M., 1984. Method to Study Antimicrobial Effects of Essential Oils: Application to the Antifungal Activity of Six Moroccan Essences. Journal of Food Protection, 47, 748-752.
  • Dülger, B. ve Hacıoğlu, N., 2008. Antifungal Activity of Endemic Salvia tigrina in Turkey. Tropical Journal of Pharmaceutical Research, 7(3), 1051-1054.
  • Erdogan, O., Celik, A. ve Zeybek, A., 2016. In Vitro Antifungal Activity of Mint, Thyme, Lavender Extracts and Essential Oils on Verticillium dahliae Kleb. Fresenius Environmental Bulletin, 25(11), 4856-4862.
  • Gökçe, A., Whalon, M.E., Çam, H., Yanar, Y., Demiştaş, İ. ve Gören, N., 2006. Plant extract contact toxicities to various developmental stages of Colorado potato beetles (Coleoptera: Chrysomelidae). Annals of applied Biology, 149, 197-202.
  • Günal, N., 1994. Liquidambar orientalis (Anadolu Sığla Ağacı)’in Güneybatı Anadolu’daki Yayılışında Relief -İklim İlişkileri. Türk Coğrafya Dergisi, 29, 175- 190.
  • Hatipoğlu, A. ve Kaşkavalcı, G., 2007. Kök-ur nematodları [Meloidogyne incognita (Kofoid&White) Chitwood]’na karşı savaşta bazı bitki kısımlarının etkileri üzerine araştırmalar. Türkiye Entomoloji Dergisi, 31 (2), 139-151.
  • Hill, A. F., 1952. Economic Botany: A Textbook of Useful Plants and Products. Vol. 2. New York: McGraw Hill; p. 560.
  • Kalhoro, M. A., Farheen, S. ve Aqsa, N.U., 2014. The Antimicrobial activity of ethanol extract of Vitex agnus castus. American International Journal of Contemporary Research, 1(1), 47-50.
  • Kalkışım, Ö., 2012. In vitro antifungal evaluation of various plant extracts against walnut anthracnose (Gnomonia leptostyla (Fr.) Ces et de Not.). Journal of Food, Agriculture & Environment, 10(3&4), 309-313.
  • Kanat, M. ve Alma, M.H., 2004. Insecticidal effects of essential oils from various plants against larvae of pine processionary moth (Thaumetopoea pityocampa Schiff) (Lepidoptera: Thaumetopoeidae). Pest management science, 60(2), 173-177.
  • Karakoç, Ö. C., Tüfekçi, A.R., Demirtaş, İ. ve İpek, A., 2013. Salvia tchihatcheffii ve Salvia cryptantha Uçucu Yağlarının ve Ekstraktlarının İki Önemli Depo Zararlısı Üzerindeki İnsektisidal Aktiviteleri. Tarım Bilimleri Araştırma Dergisi, 6 (1), 155-158.
  • Kaşkavalcı, G. ve Civelek, H.S., 2009. Effects of Two Plant Extracts on the Damage of Meloidogyne incognita in Tomato Plants. Ekoloji, 18 (72), 16-22.
  • Kim, J., Seo, S.M., Lee, S. G., Shin, S. C. ve Park, I.K., 2008. Nematicidal activity of plant essential oils and components from coriander (Coriandrum sativum), oriental sweetgum (Liquidambar orientalis), and valerian (Valeriana wallichii) essential oils against pine wood nematode (Bursaphelenchus xylophilus). Journal of Agricultural and Food Chemistry, 56(16), 7316-7320.
  • Kordali, Ş., Çakır, A., Akcin, T.A., Mete, E., Akcin, A., Aydın, T. ve Kılıç, H., 2009. Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Industrial Crops and Products, 29, 562-570.
  • Lee, Y. S., Kim, J., Lee, S. G., Oh, E., Shin, S. C. ve Park, I. K., 2009. Effects of plant essential oils and components from Oriental sweetgum (Liquidambar orientalis) on growth and morphogenesis of three phytopathogenic fungi. Pesticide Biochemistry and Physiology, 93(3), 138-143.
  • Nwosu, M. O. ve Okafor, J. l., 1995. Preliminary studies of the antifungal activites of some medicinal plants against Basidiobolus and some other pathogenic fungi. Mycoses, 38, 191-195.
  • Onaran, A., Bayan, Y. 2016. Antifungal Activity of Liquidambar orientalis L., and Myrtus communis L. Against some Plant Pathogenıc Fungi. Scientific papers-series a-agronomy, 59, 360-364.
  • Onaran, A., ve Yılar, M., 2012. Antifungal activity of Trachystemon orientalis L. aqueous extracts against plant pathogens. Journal of Food, Agriculture & Environment, 10(3&4), 287-291.

Determination of Antifungal Effects of Endemic Anatolia Sığla Tree (Liquidambar orientalis Mill.) Plant Extracts Against Some Plant Pathogenic Fungi

Year 2018, Volume: 8 Issue: 2, 202 - 208, 31.07.2018
https://doi.org/10.17714/gumusfenbil.318796

Abstract

Natural antifungal substances obtained from
plants are used as an alternative method of struggle from past to present day.
In this study, the antifungal effects of different concentrations of  methanol extract obtained from resin and
leaves of
Liquidambar orientalis Mill.
plant, endemically grown in Turkey, was investigated by using agar plate method
against fungal disease agents
Fusarium oxysporum f. sp. cucumerinum (FOC) and Monilinia fructigena plant pathogens causing damage cucumber and
apple plants, respectively were used. Mycelial growth inhibition (MGI) and
lethal doses (LD50-90) were obtained by using concentrations of
plant extarcts at 0 (negative control), 5, 10 and 20 mg/ml. Plant extract
showed antifungal effects at the all concentrations used. MGI (%) values
increased as the amount of concentration increased. Thus, the highest MGI ratio
for
L. orientalis resin is calculated
100% against
M. fructigena and 74%
for FOC. These values were 66% for
M. fructigena and 51% for FOC for L. orientalis leaf extract. In addition, LD50 values were 7.32 mg/ml for M.
fructigena, 4.42 mg/ml for FOC in case of
resin extract and 16.98 mg/ml for
M. fructigena, 7.65 mg/ml for FOC in case of leaf extract.
LD90
values were found between 7.68 mg/ml for
M. fructigena to
51.48 mg/ml for FOC in resin extract and 121.91 mg/ml for
M. fructigena to 364.59 mg/ml for FOC in leaf extract. As
a result, the resin extract against the test organisms was found to be more
effective than the leaf extract.

References

  • Ahn I.P, Chung H.S., ve Lee Y.H., 1997. Vegetative compat-ibility groups and pathogenicity among isolates of Fusarium oxysporum f. sp. cucumerinum. Plant Disease. 82, 244–246.
  • Aslan, İ. Çalmaşur, Ö., Şahin, F. ve Çağlar, Ö., 2005. Insecticidal effects of essential plant oils against Ephestia kuehniella (Zell.), Lasioderma serricorne (F.) and Sitophilus granarius (L.). Journal of Plant Diseases and Protection, 112, 257-267.
  • Basim, H., Yeğen, O. ve Zeller, W., 2000. Antibacterial effect of essential oil of T. Spicata L. var. spicata on some plant pathogenic bacteria. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, 107, 279-284.
  • Basim, E. ve Basim, H., 2013. Antibacterial activity of Turkish endemic sığla (Liquidambar orientalis Mill. var. orientalis) storax against agricultural plant pathogenic bacteria and its use as a seed protectant. Journal of Food, Agriculture & Environment, 11(3&4), 2447-2450.
  • Baydar, H., Sağdıç, O., Ozkan, G. ve Karadoğan, T., 2004. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control, 15, 169-172.
  • Benjilali, B., Tantadui-Elaraki, A., Ayadi, A. ve Ihlal, M., 1984. Method to Study Antimicrobial Effects of Essential Oils: Application to the Antifungal Activity of Six Moroccan Essences. Journal of Food Protection, 47, 748-752.
  • Dülger, B. ve Hacıoğlu, N., 2008. Antifungal Activity of Endemic Salvia tigrina in Turkey. Tropical Journal of Pharmaceutical Research, 7(3), 1051-1054.
  • Erdogan, O., Celik, A. ve Zeybek, A., 2016. In Vitro Antifungal Activity of Mint, Thyme, Lavender Extracts and Essential Oils on Verticillium dahliae Kleb. Fresenius Environmental Bulletin, 25(11), 4856-4862.
  • Gökçe, A., Whalon, M.E., Çam, H., Yanar, Y., Demiştaş, İ. ve Gören, N., 2006. Plant extract contact toxicities to various developmental stages of Colorado potato beetles (Coleoptera: Chrysomelidae). Annals of applied Biology, 149, 197-202.
  • Günal, N., 1994. Liquidambar orientalis (Anadolu Sığla Ağacı)’in Güneybatı Anadolu’daki Yayılışında Relief -İklim İlişkileri. Türk Coğrafya Dergisi, 29, 175- 190.
  • Hatipoğlu, A. ve Kaşkavalcı, G., 2007. Kök-ur nematodları [Meloidogyne incognita (Kofoid&White) Chitwood]’na karşı savaşta bazı bitki kısımlarının etkileri üzerine araştırmalar. Türkiye Entomoloji Dergisi, 31 (2), 139-151.
  • Hill, A. F., 1952. Economic Botany: A Textbook of Useful Plants and Products. Vol. 2. New York: McGraw Hill; p. 560.
  • Kalhoro, M. A., Farheen, S. ve Aqsa, N.U., 2014. The Antimicrobial activity of ethanol extract of Vitex agnus castus. American International Journal of Contemporary Research, 1(1), 47-50.
  • Kalkışım, Ö., 2012. In vitro antifungal evaluation of various plant extracts against walnut anthracnose (Gnomonia leptostyla (Fr.) Ces et de Not.). Journal of Food, Agriculture & Environment, 10(3&4), 309-313.
  • Kanat, M. ve Alma, M.H., 2004. Insecticidal effects of essential oils from various plants against larvae of pine processionary moth (Thaumetopoea pityocampa Schiff) (Lepidoptera: Thaumetopoeidae). Pest management science, 60(2), 173-177.
  • Karakoç, Ö. C., Tüfekçi, A.R., Demirtaş, İ. ve İpek, A., 2013. Salvia tchihatcheffii ve Salvia cryptantha Uçucu Yağlarının ve Ekstraktlarının İki Önemli Depo Zararlısı Üzerindeki İnsektisidal Aktiviteleri. Tarım Bilimleri Araştırma Dergisi, 6 (1), 155-158.
  • Kaşkavalcı, G. ve Civelek, H.S., 2009. Effects of Two Plant Extracts on the Damage of Meloidogyne incognita in Tomato Plants. Ekoloji, 18 (72), 16-22.
  • Kim, J., Seo, S.M., Lee, S. G., Shin, S. C. ve Park, I.K., 2008. Nematicidal activity of plant essential oils and components from coriander (Coriandrum sativum), oriental sweetgum (Liquidambar orientalis), and valerian (Valeriana wallichii) essential oils against pine wood nematode (Bursaphelenchus xylophilus). Journal of Agricultural and Food Chemistry, 56(16), 7316-7320.
  • Kordali, Ş., Çakır, A., Akcin, T.A., Mete, E., Akcin, A., Aydın, T. ve Kılıç, H., 2009. Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Industrial Crops and Products, 29, 562-570.
  • Lee, Y. S., Kim, J., Lee, S. G., Oh, E., Shin, S. C. ve Park, I. K., 2009. Effects of plant essential oils and components from Oriental sweetgum (Liquidambar orientalis) on growth and morphogenesis of three phytopathogenic fungi. Pesticide Biochemistry and Physiology, 93(3), 138-143.
  • Nwosu, M. O. ve Okafor, J. l., 1995. Preliminary studies of the antifungal activites of some medicinal plants against Basidiobolus and some other pathogenic fungi. Mycoses, 38, 191-195.
  • Onaran, A., Bayan, Y. 2016. Antifungal Activity of Liquidambar orientalis L., and Myrtus communis L. Against some Plant Pathogenıc Fungi. Scientific papers-series a-agronomy, 59, 360-364.
  • Onaran, A., ve Yılar, M., 2012. Antifungal activity of Trachystemon orientalis L. aqueous extracts against plant pathogens. Journal of Food, Agriculture & Environment, 10(3&4), 287-291.
There are 23 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Articles
Authors

Abdurrahman Onaran

Publication Date July 31, 2018
Submission Date June 6, 2017
Acceptance Date January 4, 2018
Published in Issue Year 2018 Volume: 8 Issue: 2

Cite

APA Onaran, A. (2018). Endemik Anadolu Sığla Ağacı (Liquidambar orientalis Mill. ) Bitki Ekstraktlarının Bazı Bitki Patojeni Funguslara Karşı Antifungal Etkilerinin Belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 8(2), 202-208. https://doi.org/10.17714/gumusfenbil.318796