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A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC

Year 2023, Volume: 51 Issue: 3, 289 - 295, 16.07.2023
https://doi.org/10.15671/hjbc.1270348

Abstract

This study aimed to verify the standard test method TS EN 12630 and determine the sugar contents of commercially available fruit juices by high pressure liquid chromatography. A laboratory should verify the standard test method parameters in order to show its performance for the analysis, under consideration. For this purpose, Sucrose, Glucose and Fructose were analysed in orange juices obtained from the market. The principle of the method is based on the separation of sugars on a cation-exchange resin by isocratic elution with mobile phase, detection using a differential refractive index (RI) detector and external standard method. Accuracy and precision were performed via intraday and inter day studies to determine of accuracy and the precision (generally accepted as repeatability and reproducibility) for the standard test method. The recovery values of the sugars added into juice sample were 92%, 99% and 96% for sucrose, glucose and fructose, respectively.

References

  • M.B. Heyman, S.A. Abrams, Fruit Juice in Infants, Children, and Adolescents: Current Recommendations, Pediatrics, 6 (2017) 139.
  • G.Tyagi, D. K. Jangir, P. Singh, R. Mehrotra, R. Ganesan, Rapid determination of main constituents of packed juices by reverse phase-high performance liquid chromatography: an insight in to commercial fruit drinks, Gopal J. Food Sci. Technol., 51 (2014) 476–484.
  • D.P. Rodrigues, M.L. Mitterer-Daltoé1, M.L.Vanderlei Aparecido de Lima, M.Leite,M.Barreto-Rodrigues, E.A.Pereira, C. Rural, S.Maria, Simultaneous determination of organic acids and sugars in fruit juices by High performance liquid chromatography: characterization and differentiation of commercial juices by principal component analysis, Food Technol., 51 (2021) 103-109.
  • D.K., Mykola, A.K.Volodymyr, G.G.Vitaly, M.P.Yurii, I.K. Victor, Evaluation of type 2 diabetes prevention through diet modification in people with impaired glucose regulation: A population-based study, Prim. Care Diabetes, 1 (2019) 535-541.
  • D.K.Mykola, A.K. Volodymyr, G.G.Vitaly, M.P.Yurii, I.K. Victor, Evaluation of type 2 diabetes prevention through diet modification in people with impaired glucose regulation: A population-based study, Prim. Care Diabetes, 1 (2019) 535-541.
  • C. Martínez-Villaluenga, E.Peñas, B.Hernández-Ledesma, Pseudocereal grains: Nutritional value, health benefits and current applications for the development of gluten-free foods, Food Chem. Toxicol., 137 (2020) 1-26.
  • N.Kumari, S.B.Choudhary, H.K.Sharma, B.K Singh, A.A.K. Kumar, Health-promoting properties of Corchorus leaves: A review, J. Herbal Med., 15 (2019) 1-10.
  • M.A.Faiq, G.Wollstein, J.S. Schuman, K.C. Chan, Cholinergic nervous system and glaucoma: From basic science to clinical applications, Prog. Retin. Eye Res., 72 (2019) 1-27.
  • K.Dutta, D.A.Podolin, M.B.Davidson, A.J.Davidoff, Cardiomyocyte dysfunction in sucrose-fed rats is associated with insulin resistance, Am. Diabetes Assoc., 50 (2001) 1186-1192.
  • Q.Gan, J.Wang, J.Hu, G.Lou, H.Xiong, C.Peng, S.Heng, Q.Huang, The role of diosgenin in diabetes and diabetic complications, J. Steroid. Biochem. Mol. Biol., 198 (2019) 105575.
  • H. Yan Wang, Q.Ming Li, N.Jun Yu, W.Dong Chen, X.Qiang Zha, D. De-Ling Wu, L.Li-Hua Pan, Jun Duan, J.Ping Luo, Dendrobium huoshanense polysaccharide regulates hepatic glucose homeostasis and pancreatic β-cell function in type 2 diabetic mice, Carbohydr. Polym., 211 (2019) 39-48.
  • V. Armoogum, K. Boodhoo, Full Optımızatıon And Valıdatıon Of An Hplc Method For The Quantıtatıve Analysıs Of Total Sugars In A Soft Drınk, Bull. Chem. Soc. Ethiop., 34 (2020) 419-426.
  • A.J.Buglass, Chemical Composition of Beverages and Drinks, HandBook of Food Chem., Springer-Verlag, Berlin Heidelberg, 2015.
  • A.De Olivera, F. e Castilhos, C.M.G.C.Renard, S. Bureau, Comparison of NIR and MIR spectroscopic methods for determination of individual sugars, organic acids and carotenoids in passion fruit, Food Res. Intern., 60 (2014) 154-162.
  • M.P.Costa, B.S. Frasao, B. R. C. da Costa Lima, B. L. Rodrigues, C. A. C. Junior, Simultaneous analysis of carbohydrates and organic acids by HPLC- DAD-RI for monitoring goat’s milk yogurts fermentation, Talanta, 152 (2016) 162-170.
  • I. M.Demiate, G.Wosiacki, C. Czelusniak, A.Nogueira, Analysis of total and reducing sugar infoods. A comparative study between colorimetric and titration techniques, Ciências Agrárias e Engenharias, 8 (2002) 65-78.
  • A.H. Jahren, C. Saudek, E.H. Yeung, W.H. Kao, R.A. Kraft, B. Caballero, An isotopic method for quantifying sweeteners derived from corn and sugar cane, Am. J. Clin. Nutr., 84 (2006) 1380-4.
  • TS EN12630 (Fruit and Vegetable Juices-Glucose, Fructose, Sorbitol and Determination of Sucharose Content-High Performance Liquid Chromatography Method), UNE EN 12630:2000, (2000) 01-21.
  • “How to Meet ISO 17025 Requirements for Method Verification-2007 ALACC Guide” AOAC (Association of Analytical Communities) How to Meet ISO 17025 Requirements for Method Verification- ALACC Guide, (2007).
  • M. F. Neves, V. G. Trombin, V. Marques, L. F. Martinez, Global orange juice market: a 16-year summary and opportunities for creating value, Trop. Plant Pathol., 45 (2020) 166–174.

Ticari Meyve Sularında HPLC ile Şeker Analizi için Standart Bir Metot Teyidi

Year 2023, Volume: 51 Issue: 3, 289 - 295, 16.07.2023
https://doi.org/10.15671/hjbc.1270348

Abstract

Bu çalışmada, TS EN 12630 standart metodunun doğrulanması ve piyasada bulunan meyve sularının şeker içeriklerinin yüksek basınçlı sıvı kromatografisi ile belirlenmesi amaçlanmıştır. Laboratuvar söz konusu standart test metodundaki performansını göstermek için, standart metodun parametrelerini teyit etmelidir. Bu amaçla piyasadan temin edilen portakal sularında Sükroz, Glikoz ve Fruktoz analizi yapılmıştır. Metodun prensibi, şekerlerin hareketli faz yardımı ile izokratik elüsyon ile bir katyon-değiştirici reçine üzerinde ayrılması, diferansiyel kırılma indisi (RI) dedektörü ve dış standart metodu kullanılarak saptanması esasına dayanmaktadır. Gün içi ve günler arası analizler ile yöntemin doğruluk ve kesinliği belirlenerek metot verifikasyonu gerçekleştirilmiştir. Meyve suyu örneğine eklenen şekerlerin geri kazanım değerleri sukroz, glikoz ve fruktoz için sırasıyla %92, %99 ve %96 bulunmuştur.

References

  • M.B. Heyman, S.A. Abrams, Fruit Juice in Infants, Children, and Adolescents: Current Recommendations, Pediatrics, 6 (2017) 139.
  • G.Tyagi, D. K. Jangir, P. Singh, R. Mehrotra, R. Ganesan, Rapid determination of main constituents of packed juices by reverse phase-high performance liquid chromatography: an insight in to commercial fruit drinks, Gopal J. Food Sci. Technol., 51 (2014) 476–484.
  • D.P. Rodrigues, M.L. Mitterer-Daltoé1, M.L.Vanderlei Aparecido de Lima, M.Leite,M.Barreto-Rodrigues, E.A.Pereira, C. Rural, S.Maria, Simultaneous determination of organic acids and sugars in fruit juices by High performance liquid chromatography: characterization and differentiation of commercial juices by principal component analysis, Food Technol., 51 (2021) 103-109.
  • D.K., Mykola, A.K.Volodymyr, G.G.Vitaly, M.P.Yurii, I.K. Victor, Evaluation of type 2 diabetes prevention through diet modification in people with impaired glucose regulation: A population-based study, Prim. Care Diabetes, 1 (2019) 535-541.
  • D.K.Mykola, A.K. Volodymyr, G.G.Vitaly, M.P.Yurii, I.K. Victor, Evaluation of type 2 diabetes prevention through diet modification in people with impaired glucose regulation: A population-based study, Prim. Care Diabetes, 1 (2019) 535-541.
  • C. Martínez-Villaluenga, E.Peñas, B.Hernández-Ledesma, Pseudocereal grains: Nutritional value, health benefits and current applications for the development of gluten-free foods, Food Chem. Toxicol., 137 (2020) 1-26.
  • N.Kumari, S.B.Choudhary, H.K.Sharma, B.K Singh, A.A.K. Kumar, Health-promoting properties of Corchorus leaves: A review, J. Herbal Med., 15 (2019) 1-10.
  • M.A.Faiq, G.Wollstein, J.S. Schuman, K.C. Chan, Cholinergic nervous system and glaucoma: From basic science to clinical applications, Prog. Retin. Eye Res., 72 (2019) 1-27.
  • K.Dutta, D.A.Podolin, M.B.Davidson, A.J.Davidoff, Cardiomyocyte dysfunction in sucrose-fed rats is associated with insulin resistance, Am. Diabetes Assoc., 50 (2001) 1186-1192.
  • Q.Gan, J.Wang, J.Hu, G.Lou, H.Xiong, C.Peng, S.Heng, Q.Huang, The role of diosgenin in diabetes and diabetic complications, J. Steroid. Biochem. Mol. Biol., 198 (2019) 105575.
  • H. Yan Wang, Q.Ming Li, N.Jun Yu, W.Dong Chen, X.Qiang Zha, D. De-Ling Wu, L.Li-Hua Pan, Jun Duan, J.Ping Luo, Dendrobium huoshanense polysaccharide regulates hepatic glucose homeostasis and pancreatic β-cell function in type 2 diabetic mice, Carbohydr. Polym., 211 (2019) 39-48.
  • V. Armoogum, K. Boodhoo, Full Optımızatıon And Valıdatıon Of An Hplc Method For The Quantıtatıve Analysıs Of Total Sugars In A Soft Drınk, Bull. Chem. Soc. Ethiop., 34 (2020) 419-426.
  • A.J.Buglass, Chemical Composition of Beverages and Drinks, HandBook of Food Chem., Springer-Verlag, Berlin Heidelberg, 2015.
  • A.De Olivera, F. e Castilhos, C.M.G.C.Renard, S. Bureau, Comparison of NIR and MIR spectroscopic methods for determination of individual sugars, organic acids and carotenoids in passion fruit, Food Res. Intern., 60 (2014) 154-162.
  • M.P.Costa, B.S. Frasao, B. R. C. da Costa Lima, B. L. Rodrigues, C. A. C. Junior, Simultaneous analysis of carbohydrates and organic acids by HPLC- DAD-RI for monitoring goat’s milk yogurts fermentation, Talanta, 152 (2016) 162-170.
  • I. M.Demiate, G.Wosiacki, C. Czelusniak, A.Nogueira, Analysis of total and reducing sugar infoods. A comparative study between colorimetric and titration techniques, Ciências Agrárias e Engenharias, 8 (2002) 65-78.
  • A.H. Jahren, C. Saudek, E.H. Yeung, W.H. Kao, R.A. Kraft, B. Caballero, An isotopic method for quantifying sweeteners derived from corn and sugar cane, Am. J. Clin. Nutr., 84 (2006) 1380-4.
  • TS EN12630 (Fruit and Vegetable Juices-Glucose, Fructose, Sorbitol and Determination of Sucharose Content-High Performance Liquid Chromatography Method), UNE EN 12630:2000, (2000) 01-21.
  • “How to Meet ISO 17025 Requirements for Method Verification-2007 ALACC Guide” AOAC (Association of Analytical Communities) How to Meet ISO 17025 Requirements for Method Verification- ALACC Guide, (2007).
  • M. F. Neves, V. G. Trombin, V. Marques, L. F. Martinez, Global orange juice market: a 16-year summary and opportunities for creating value, Trop. Plant Pathol., 45 (2020) 166–174.
There are 20 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Tuğba Doğan 0000-0003-2892-1541

Tamay Şeker 0000-0001-7606-7226

Early Pub Date July 14, 2023
Publication Date July 16, 2023
Acceptance Date June 8, 2023
Published in Issue Year 2023 Volume: 51 Issue: 3

Cite

APA Doğan, T., & Şeker, T. (2023). A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC. Hacettepe Journal of Biology and Chemistry, 51(3), 289-295. https://doi.org/10.15671/hjbc.1270348
AMA Doğan T, Şeker T. A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC. HJBC. July 2023;51(3):289-295. doi:10.15671/hjbc.1270348
Chicago Doğan, Tuğba, and Tamay Şeker. “A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC”. Hacettepe Journal of Biology and Chemistry 51, no. 3 (July 2023): 289-95. https://doi.org/10.15671/hjbc.1270348.
EndNote Doğan T, Şeker T (July 1, 2023) A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC. Hacettepe Journal of Biology and Chemistry 51 3 289–295.
IEEE T. Doğan and T. Şeker, “A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC”, HJBC, vol. 51, no. 3, pp. 289–295, 2023, doi: 10.15671/hjbc.1270348.
ISNAD Doğan, Tuğba - Şeker, Tamay. “A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC”. Hacettepe Journal of Biology and Chemistry 51/3 (July 2023), 289-295. https://doi.org/10.15671/hjbc.1270348.
JAMA Doğan T, Şeker T. A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC. HJBC. 2023;51:289–295.
MLA Doğan, Tuğba and Tamay Şeker. “A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC”. Hacettepe Journal of Biology and Chemistry, vol. 51, no. 3, 2023, pp. 289-95, doi:10.15671/hjbc.1270348.
Vancouver Doğan T, Şeker T. A Standard Method Verification for Determination of Sugar Content of Commercial Fruit Juices by HPLC. HJBC. 2023;51(3):289-95.

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