Glaucium Cappadocicum: Screening on Antioxidant, Antimicrobial, Anticholinesterase Inhibition in vitro

Year 2024, Volume: 52 Issue: 1, 1 - 9, 04.01.2024

Sevgi Altın Ekrem Köksal

https://doi.org/10.15671/hjbc.1332418

Abstract

Glaucium cappadocicum is an endemic plant in Turkey, but there have been no research on it. In this work, the total phenolic and flavonoid content of extracts made from this plant were measured. Additionally, their antioxidant, antibacterial, and anticholinesterase activities were assessed. The extracts' antioxidant properties were assessed using the DPPH and FRAP methods, their antibacterial effects were assessed using the disk diffusion method on Gram-positive and Gram-negative bacteria, and their ability to inhibit cholinesterase activity was assessed using the Ellman method. In all biological activity assays, the alkaloid extract had the maximum activity. Alkaloid extract shown higher inhibition than the common inhibitor Tacrine in investigations on enzyme inhibition, in particular. All of these investigations demonstrate the potent anticholinesterase and antibacterial properties of Glaucium cappadocicum as well as its value as a good source of antioxidant system defense.

Keywords

Glaucium cappadocicum, enzyme inhibition, antimicrobial, antioxidant

References

• M. Ghorbanpour, J. Hadian, S.Nikabadi & A. Varma. Importance of medicinal and aromatic plants in human life. Medicinal plants and environmental challenges, (2017) 1-23.
• Ş.Yıldırımlı. Türkiye’nin jipizçin bitki çeşitliliği cenneti: Kepen, Sivrihisar, Eskişehir, 13 yeni üye, Türkiye. OT Sistematik Botanik Dergisi 19 (2012) 34-38.
• C.Aykurt, K.Yıldız, A. Özçandır, F.Mungan, G. Deniz. Glaucium alakirensis (Papaveraceae), a new species from Southern Anatolia, Turkey. Phytotaxa., 295.3 (2017) 255-262.
• M.E. Mohamed, A.M. Arafa, S.S. Soliman, S.I. Eldahmy. Plant germination and production of callus from the yellow hornpoppy (Glaucium flavum): the first stage of micropropagation. Pharmazie, 69.9 (2014) 715-720.
• A.M. Arafa, M.E.S. Mohamed, S.I. Eldahmy. The aerial parts of yellow horn poppy (Glaucium flavum Cr.) growing in Egypt: Isoquinoline alkaloids and biological activities. J. Pharm. Sci. Res.. 8.5 (2016) 323.
• T. Akaberi, K. Shourgashti, S.A. Emami., M. Akaberi. Phytochemistry and pharmacology of alkaloids from Glaucium spp. Phytochemistry., 191 (2021) 112923.
• Z. Ibn Beyṭar. Al-Jameʿe le-Mofradat al-Adwiah wal-Aghdhiyah (comprehensive book in simple drugs and foods in Arabic). Dar al-Kutub al-ʿElmiyah, Beirut (1422).
• A. A. K. H. M Shirâzi. M.S. Ardakani, R. Rahimi, F. Farjadmand .Makhzan al-Adwyah (drug treasure). Tehran: Sabz Arang Publisher (2014) 281-304.
• A. Elbermawi, A. Sallam, H.A. Ghabbour, M.F. Elsebai, M.F. Lahloub, H. E. A. Saad. Bioactive isoquinoline alkaloids from Glaucium arabicum. Phytochem Lett., 28 (2018) 139-144.
• K. Morteza-Semnani, M. Saeedi, and M. R. Mahdavi. Antibacterial studies on extracts of three species of Glaucium. from Iran. Pharm Biol., 43.3 (2005) 234-236.
• I.A. Abdel-Hassan, J.A. Abdel-Barry, and S. T. Mohammeda. The hypoglycaemic and antihyperglycaemic effect of Citrullus colocynthis fruit aqueous extract in normal and alloxan diabetic rabbits. J.Ethnopharmacol., 71.1-2 (2000) 325-330.
• S. Zheng, and S. Zheng. Bocconoline from Glaucium fimbrilligerum Boiss. Induces Apoptosis of Human Breast Cancer MCF-7 Cells via Mitochondria-Dependent Pathway. Lat. Am. J. Pharm., 39.10 (2020) 2022-2028.
• F. Kocanci, B. Hamamcioglu, and B. Aslım. The anti-AChE and anti-proliferative Activities of Glaucium acutidentatum and Glaucium corniculatum Alkaloid Extracts. J. Appl. Pharm. Sci., 7.8 (2017).
• N. Ozsoy, T. Yılmaz-Ozden, P. Aksoy-Sagirli, H. Şahin, & A. Sarı. Antioxidant, Anti-acetylcholinesterase, Anti-inflammatory and DNA Protection Activities of Glaucium grandiflorum var. grandiflorum. Iran J.Pharm.Res: IJPR 17.2 (2018) 677.
• E. Rangriz, Z. Mousavi, P. Najafizadeh, & J. Asgarpanah. Antinociceptive effect of the endemic species Glaucium vitellinum boiss and buhse. Jundishapur J Nat Pharm Prod., 32.6 (2016).
• R.Suau, B. Cabezudo, R. Rico, F. Nájera, J.M. López-Romero, & A. Cuevas. Phytochemical variations within populations of Platycapnos saxicola Willk. Biochem. Syst. Ecol., 32.6 (2004) 565-572.
• S. Altın, S. Akyüz, E.K.K. Yeniçeri, E. Köksal., A. Altay, & C. Alp. Investigation of Antimicrobial Activity of Lallemantia canescens (L) Fisch & Mey. Erzincan Üniv. Fen Bilim. Enst. derg., 14.2 (2021) 849-856.
• S. Altın, C. Alp, E. Köksal, & S. Akyüz. Determination of Antioxidant, Antimicrobial, and Antiproliferative Activities of Onobrychis argyrea subsp. argyrea Extracts. J. Sci. Technol., 13.2 (2023) 1134-1141.
• M.S. Blois. Antioxidant determinations by the use of a stable free radical. Nature 181.4617 (1958) 1199-1200.
• M. Oyaizu. Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Jap. J. Nutr., 44 (1986) 307-315.
• K. Slinkard, and V.L. Singleton. Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic., 28.1 (1977) 49-55.
• C.C. Chang, M.H. Yang, H.M. Wen, & J.C. Chern. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal., 10.3 (2002).
• A.W. Bauer, W.M.M. Kirby, J.C. Sherris, & M. Turck. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 45.4_ts (1966) 493-496.
• G.L. Ellman, K.D. Courtney, V. Andres, & R.M. Featherstone, A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol., 7.2 (1961) 88-95.
• İ. Gulçin, M. Abbasova, P. Taslimi, Z. Huyut, L. Safarova, A. Sujayev, V. Farzaliyev, Ş. Beydemir ,S.H. Alwasel , & C.T. Supuran. Synthesis and biological evaluation of aminomethyl and alkoxymethyl derivatives as carbonic anhydrase, acetylcholinesterase and butyrylcholinesterase inhibitors. J Enzyme Inhib Med Chem., 32.1 (2017) 1174-1182.
• S. Burmaoglu, A.O. Yilmaz, M.F. Polat, R. Kaya, İ. Gulcin, & O. Algul. Synthesis and biological evaluation of novel tris-chalcones as potent carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase and α-glycosidase inhibitors. Bioorg. Chem., 85 (2019) 191-197.
• H. Cavdar, M. Senturk, M. Guney, S. Durdagi, G. Kayik, C.T. Supuran, & D. Ekinci. Inhibition of acetylcholinesterase and butyrylcholinesterase with uracil derivatives: kinetic and computational studies. J Enzyme Inhib Med Chem., 34.1 (2019) 429-437.
• N. Eruygur, U.M. Koçyiğit, P. Taslimi, M. Ataş, M. Tekin, & İ. Gülçin. Screening the in vitro antioxidant, antimicrobial, anticholinesterase, antidiabetic activities of endemic Achillea cucullata (Asteraceae) ethanol extract. S. Afr. J. Bot., 120 (2019) 141-145.
• M. Boulaaba, F.Z. Kalai, S. Dakhlaoui, Y. Ezzine, S. Selmi, S. Bourgou, A. Smaoui, H. Isoda & R. Ksouri. Antioxidant, antiproliferative and anti-inflammatory effects of Glaucium flavum fractions enriched in phenolic compounds. Med Chem., 28.11 (2019) 1995-2001.
• A. Önder, A.S. Çınar, G. Gençaslan, & T. Çoban. Antioxidant potentials of the extracts from 14 selected medicinal plants. J. med. herbs ethnomed., 6 (2020) 19.
• A. Gülbandılar, Kütahya Yöresinde Burun Mukozasındaki Staphylococcus Aureus Taşıyıcılığının ve Antibiyotik Duyarlılığının Araştırılması. Journal of Science and Technology of Dumlupınar University 018 (2009) 1-6.
• J.P. Rauha, S. Remes, M. Heinonen, A. Hopia, M. Kahkonen, T. Kujala, K. Pihlaja, H. Vuorela, H., & P. Vuorela. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int. J. Food Microbiol., 56 (2000) 3–12.
• M. Hakemi-Vala, M. Mehrara, M. Pourramezan, J. Asgarpanah, N. Rahimifard, S. Khoshnood, & M. Heidary. Comparison the antimicrobial effects of the flowering aerial parts of Glaucium vitellinum Boiss. and Buhse and Gaillonia aucheri Jaub. and Spach. Novelty in Biomedicine 5.1 (2017).
• E.H. Soureshjan, and M. Heidari. In vitro variation in antibacterial activity plant extracts on Glaucium elegans and saffron (Crocus sativus). Bangladesh J Pharmacol., 9.3 (2014) 275-278.
• K. Kucukoglu, H.I. Gul, P. Taslimi, İ. Gulcin, & C.T. Supuran. Investigation of inhibitory properties of some hydrazone compounds on hCA I, hCA II and AChE enzymes. Bioorg. Chem., 86 (2019) 316-321.