Research Article
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Year 2020, Volume: 7 Issue: 2, 571 - 580, 23.06.2020
https://doi.org/10.18596/jotcsa.646433

Abstract

References

  • 1. McCord JM and Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry. 1969 Nov; 244: 6049–55.
  • 2. Halliwell B and Gutteridge JMC. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochemical Journal. 1984 Apr; 219: 1–14.
  • 3. Kehrer JP. The Haber–Weiss reaction and mechanisms of toxicity. Toxicology. 2000 Aug; 149(1): 43–50.
  • 4. Auchere F and Rusnak F. What is the ultimate fate of superoxide anion in vivo? Journal of Biological Inorganic Chemistry 2002 Jun; 7: 664–7.
  • 5. Wrona MZ and Dryhurst G. Oxidation of serotonin by superoxide radical: implications to neurodegenerative brain disorders. Chemical Research in Toxicology. 1998 Jun; 11(6): 639–50.
  • 6. Vanella A, Di Giacomo C, Sorrenti V, Russo A, Castorina C, Campisi A, Renis M. and Perez-Polo JR. Free radical scavenger depletion in post-ischemic reperfusion brain damage. Neurochemical Research. 1993 Dec; 18(12): 1337–40.
  • 7. Cadenas E and Davies KJA. Mitochondrial free radical generation, oxidative stress, and aging. Free Radical Biology and Medicine. 2000 Aug; 29(3-4): 222–30.
  • 8. Floyd RA. Role of oxygen free radicals in carcinogenesis and brain ischemia. The FASEB Journal. 1990 Jun; 4(9): 2587–97.
  • 9. Kumar H, Lim H-W, More SV, Kim B-W, Koppula S, Kim IS, and Choi D-K. The role of free radicals in the aging brain and Parkinson’s disease: Convergence and parallelism. International Journal of Molecular Sciences. 2012 Aug; 13(8): 10478–504.
  • 10. Zhou Y, Ding J, Liang T, Abdel-Halim ES, Jiang L, Zhu J-J. FITC doped rattle-type silica colloidal particle-based ratiometric fluorescent sensor for biosensing and imaging of superoxide anion. ACS Applied Materials and Interfaces. 2016 Feb; 8: 6423−30.
  • 11. Yildirim O, Derkus B. Triazine-based 2D covalent organic frameworks improve the electrochemical performance of enzymatic biosensors. Journal of Materials Science. 2020; 55:3034-44.
  • 12. Emregul E. Development of a new biosensor for superoxide radicals. Analytical and Bioanalytical Chemistry. 2005 Nov; 383: 947-54.
  • 13. Campanella L, Favero G, Persi L, Tomassetti M. New biosensor for superoxide radical used to evidence molecules of biomedical and pharmaceutical interest having radical scavenging properties. Journal of Pharmaceutical and Biomedical Analysis. 2000; 23: 69-76.
  • 14. Rahimi P, Ghourchian H, Refiee-Pour H-A. Superoxide radical biosensor based on a nano-composite containing cytochrome c. Analyst. 2011 June; 136: 3803-8.
  • 15. Braik M, Barsan MM, Dridi C, Ali MB, Brett CMA. Highly sensitive amperometric enzyme biosensor for detection of superoxide based on conducting polymer/CNT modified electrodes and superoxide dismutase. Sensors and Actuators B. 2016 June; 236:574-82.
  • 16. Crulhas BP, Recco LC, Delella FK, Pedrosa VA. A novel superoxide anion biosensor for monitoring reactive species of oxygen released by cancer cells. Electroanalysis. 2017; 29:1-7.
  • 17. Derkus B, Acar Bozkurt P. Multilayer graphene oxide-silver nanoparticle nanostructure as efficient peroxidase mimic. Hacettepe Journal of Biology and Chemistry. 2018; 46(2): 159-67.
  • 18. Yilmaz MS, Derkus B, Emregul E. Investigation of the use of collagen-gelatin-gold nanoparticle nanocomposite system as an aptasensor matrix. Hacettepe Journal of Biology and Chemistry. 2019; 46(4): 523-31.
  • 19. Derkus B, Emregul E, Emregul KC. Copper–zinc alloy nanoparticle based enzyme-free superoxide radical sensing on a screen-printed electrode. Talanta. 2015;134:206-14.
  • 20. Derkus B, Emregul E, Emregul KC, Yucesan C. Alginate and alginate-titanium dioxide nanocomposite as electrode materials for anti-myelin basic protein immunosensing. Sensors and Actuators B. 2014; 192: 294-302.
  • 21. Bao S-J, Li CM, Zang J-F, Cui X-Q, Qiao Y, Guo J. New nanostructured TiO2 for direct electrochemistry and glucose sensor applications. Advanced Functional Materials. 2008 Feb; 18(4): 591-9.
  • 22. Luo Y, Liu H, Rui Q, Tian Y. Detection of Extracellular H2O2 Released from Human Liver Cancer Cells Based on TiO2 Nanoneedles with Enhanced Electron Transfer of Cytochrome c. Analytical Chemistry. 2009 March; 81(8): 3035-41.
  • 23. Emregul E, Kocabay O, Derkus B, Yumak T, Emregul KC, Sinag A, Polat K. A novel carboxymethylcellulose–gelatin–titanium dioxide–superoxide dismutase biosensor; electrochemical properties of carboxymethylcellulose–gelatin–titanium dioxide–superoxide dismutase. Bioelectrochemistry. 2013 Apr; 90:8-17. 24.Wang X, Han M, Bao J, Tu W and Dai Z. A superoxide anion biosensor based on direct electron transfer of superoxide dismutase on sodium alginate sol–gel film and its application to monitoring of living cells. Analytica Chimica Acta. 2012; 717: 61– 6.
  • 25. Liou G-Y, Storz P. Reactive oxygen species in cancer. Free Radical Research. 2010 May; 44(5): 479-96.
  • 26. Keshavarzian A, Zapeda D, List T, Mobarhan S. High levels of reactive oxygen metabolites in colon cancer tissue: Analysis by chemiluminescence prob. Nutrition and Cancer. 1992 Jan; 17(3): 243-9.
  • 27. Mavrikou S, Tsekouras V, Karageorgou M-A, Moschopoulou G, Kintzios S. Detection of superoxide alterations induced by 5-Fluorouracil on HeLa cells with a cell-based biosensor. Biosensors. 2019 Oct; 9(4):126-38.
  • 28. Han M, Guo P, Wang X, Tu W, Bao J, Dai Z. Mesoporous SiO2–(L)-lysine hybrid nanodisks: direct electron transfer of superoxide dismutase, sensitive detection of superoxide anions and its application in living cell monitoring. RSC Advances. 2013 Aug; 3(43): 20456-63.
  • 29. Tang J, Zhu X, Niu X, Liu T, Zhao H, Lan M. Anamperometric superoxide anion radicalbiosensor based on SOD/PtPd-PDARGO modified electrode. Talanta. 2015 Jan; 137: 18-24.
  • 30. Ye Q, Li W, Wang Z, Zhang L, Tan X, Tian Y. Direct electrochemistry of superoxide dismutases (Mn-, Fe-, and Ni-) from human pathogen Clostridium difficile: Toward application to superoxide biosensor. Journal of Electroanalytical Chemistry. 2014 July; 729: 21-6.

Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals

Year 2020, Volume: 7 Issue: 2, 571 - 580, 23.06.2020
https://doi.org/10.18596/jotcsa.646433

Abstract

In this work, a biosensor that uses
gelatin and alginate hydrogels in addition to titanium dioxide (TiO2)
nanoparticles (NPs) as sensor matrix was developed in order to detect
superoxide radicals (O2•-), which play role in carcinogenesis when
present in excess levels. Parameters affecting the performance of the biosensor
such as amount of gelatin-alginate ratio, amount of TiO2 NPs,
concentration of SOD enzymes and glutaraldehyde cross-linker were investigated.
Electrochemical Impedance Spectroscopy (EIS) and chronoamperometry were used as
electrochemical technique for the development of biosensor as well as
characterisation steps. The developed biosensor exhibited two linear ranges
between 0.0009 mM – 0.125 mM and 0.25 mM – 2 mM which were utilized as
calibration curves. Detection limit of the biosensor was found 0.9 μM, which
was at appropriate level for the detection of O2•- in tumour
samples. Finally, the constructed biosensor showed significant analytical performance
such as high selection for
O2•-,
low detection limit, and long-term stability.

References

  • 1. McCord JM and Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry. 1969 Nov; 244: 6049–55.
  • 2. Halliwell B and Gutteridge JMC. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochemical Journal. 1984 Apr; 219: 1–14.
  • 3. Kehrer JP. The Haber–Weiss reaction and mechanisms of toxicity. Toxicology. 2000 Aug; 149(1): 43–50.
  • 4. Auchere F and Rusnak F. What is the ultimate fate of superoxide anion in vivo? Journal of Biological Inorganic Chemistry 2002 Jun; 7: 664–7.
  • 5. Wrona MZ and Dryhurst G. Oxidation of serotonin by superoxide radical: implications to neurodegenerative brain disorders. Chemical Research in Toxicology. 1998 Jun; 11(6): 639–50.
  • 6. Vanella A, Di Giacomo C, Sorrenti V, Russo A, Castorina C, Campisi A, Renis M. and Perez-Polo JR. Free radical scavenger depletion in post-ischemic reperfusion brain damage. Neurochemical Research. 1993 Dec; 18(12): 1337–40.
  • 7. Cadenas E and Davies KJA. Mitochondrial free radical generation, oxidative stress, and aging. Free Radical Biology and Medicine. 2000 Aug; 29(3-4): 222–30.
  • 8. Floyd RA. Role of oxygen free radicals in carcinogenesis and brain ischemia. The FASEB Journal. 1990 Jun; 4(9): 2587–97.
  • 9. Kumar H, Lim H-W, More SV, Kim B-W, Koppula S, Kim IS, and Choi D-K. The role of free radicals in the aging brain and Parkinson’s disease: Convergence and parallelism. International Journal of Molecular Sciences. 2012 Aug; 13(8): 10478–504.
  • 10. Zhou Y, Ding J, Liang T, Abdel-Halim ES, Jiang L, Zhu J-J. FITC doped rattle-type silica colloidal particle-based ratiometric fluorescent sensor for biosensing and imaging of superoxide anion. ACS Applied Materials and Interfaces. 2016 Feb; 8: 6423−30.
  • 11. Yildirim O, Derkus B. Triazine-based 2D covalent organic frameworks improve the electrochemical performance of enzymatic biosensors. Journal of Materials Science. 2020; 55:3034-44.
  • 12. Emregul E. Development of a new biosensor for superoxide radicals. Analytical and Bioanalytical Chemistry. 2005 Nov; 383: 947-54.
  • 13. Campanella L, Favero G, Persi L, Tomassetti M. New biosensor for superoxide radical used to evidence molecules of biomedical and pharmaceutical interest having radical scavenging properties. Journal of Pharmaceutical and Biomedical Analysis. 2000; 23: 69-76.
  • 14. Rahimi P, Ghourchian H, Refiee-Pour H-A. Superoxide radical biosensor based on a nano-composite containing cytochrome c. Analyst. 2011 June; 136: 3803-8.
  • 15. Braik M, Barsan MM, Dridi C, Ali MB, Brett CMA. Highly sensitive amperometric enzyme biosensor for detection of superoxide based on conducting polymer/CNT modified electrodes and superoxide dismutase. Sensors and Actuators B. 2016 June; 236:574-82.
  • 16. Crulhas BP, Recco LC, Delella FK, Pedrosa VA. A novel superoxide anion biosensor for monitoring reactive species of oxygen released by cancer cells. Electroanalysis. 2017; 29:1-7.
  • 17. Derkus B, Acar Bozkurt P. Multilayer graphene oxide-silver nanoparticle nanostructure as efficient peroxidase mimic. Hacettepe Journal of Biology and Chemistry. 2018; 46(2): 159-67.
  • 18. Yilmaz MS, Derkus B, Emregul E. Investigation of the use of collagen-gelatin-gold nanoparticle nanocomposite system as an aptasensor matrix. Hacettepe Journal of Biology and Chemistry. 2019; 46(4): 523-31.
  • 19. Derkus B, Emregul E, Emregul KC. Copper–zinc alloy nanoparticle based enzyme-free superoxide radical sensing on a screen-printed electrode. Talanta. 2015;134:206-14.
  • 20. Derkus B, Emregul E, Emregul KC, Yucesan C. Alginate and alginate-titanium dioxide nanocomposite as electrode materials for anti-myelin basic protein immunosensing. Sensors and Actuators B. 2014; 192: 294-302.
  • 21. Bao S-J, Li CM, Zang J-F, Cui X-Q, Qiao Y, Guo J. New nanostructured TiO2 for direct electrochemistry and glucose sensor applications. Advanced Functional Materials. 2008 Feb; 18(4): 591-9.
  • 22. Luo Y, Liu H, Rui Q, Tian Y. Detection of Extracellular H2O2 Released from Human Liver Cancer Cells Based on TiO2 Nanoneedles with Enhanced Electron Transfer of Cytochrome c. Analytical Chemistry. 2009 March; 81(8): 3035-41.
  • 23. Emregul E, Kocabay O, Derkus B, Yumak T, Emregul KC, Sinag A, Polat K. A novel carboxymethylcellulose–gelatin–titanium dioxide–superoxide dismutase biosensor; electrochemical properties of carboxymethylcellulose–gelatin–titanium dioxide–superoxide dismutase. Bioelectrochemistry. 2013 Apr; 90:8-17. 24.Wang X, Han M, Bao J, Tu W and Dai Z. A superoxide anion biosensor based on direct electron transfer of superoxide dismutase on sodium alginate sol–gel film and its application to monitoring of living cells. Analytica Chimica Acta. 2012; 717: 61– 6.
  • 25. Liou G-Y, Storz P. Reactive oxygen species in cancer. Free Radical Research. 2010 May; 44(5): 479-96.
  • 26. Keshavarzian A, Zapeda D, List T, Mobarhan S. High levels of reactive oxygen metabolites in colon cancer tissue: Analysis by chemiluminescence prob. Nutrition and Cancer. 1992 Jan; 17(3): 243-9.
  • 27. Mavrikou S, Tsekouras V, Karageorgou M-A, Moschopoulou G, Kintzios S. Detection of superoxide alterations induced by 5-Fluorouracil on HeLa cells with a cell-based biosensor. Biosensors. 2019 Oct; 9(4):126-38.
  • 28. Han M, Guo P, Wang X, Tu W, Bao J, Dai Z. Mesoporous SiO2–(L)-lysine hybrid nanodisks: direct electron transfer of superoxide dismutase, sensitive detection of superoxide anions and its application in living cell monitoring. RSC Advances. 2013 Aug; 3(43): 20456-63.
  • 29. Tang J, Zhu X, Niu X, Liu T, Zhao H, Lan M. Anamperometric superoxide anion radicalbiosensor based on SOD/PtPd-PDARGO modified electrode. Talanta. 2015 Jan; 137: 18-24.
  • 30. Ye Q, Li W, Wang Z, Zhang L, Tan X, Tian Y. Direct electrochemistry of superoxide dismutases (Mn-, Fe-, and Ni-) from human pathogen Clostridium difficile: Toward application to superoxide biosensor. Journal of Electroanalytical Chemistry. 2014 July; 729: 21-6.
There are 29 citations in total.

Details

Primary Language English
Subjects Chemical Engineering
Journal Section Articles
Authors

Utku Karakaya This is me 0000-0002-0970-7622

Burak Derkuş 0000-0001-5558-0995

Emel Emregul This is me 0000-0002-7892-6730

Publication Date June 23, 2020
Submission Date November 13, 2019
Acceptance Date May 26, 2020
Published in Issue Year 2020 Volume: 7 Issue: 2

Cite

Vancouver Karakaya U, Derkuş B, Emregul E. Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals. JOTCSA. 2020;7(2):571-80.