The
breast cancer has become a threat to women's lives at an increasing rate. The
inadequate treatments of surgical procedures and chemotherapy affect negatively
quality of patient life. Therefore, there has been an increase in demand for
alternative and supportive agents, especially for the use of herbal products.
The purpose of this study is to evaluate the in vitro anti-tumor activity of Lysimachia savranii in breast cancer
cell lines compared to normal adipose tissue derived mesenchymal stem cells. The
adipose tissue derived mesenchymal stem cells as normal cells with MCF-7 and,
MDA-MB-231 breast cancer cells were cultured. IC50 doses of Lysimachia savranii extract for each
cells were determined via MTT assay. The immunocytochemical staining was
performed for the oxidative stress by eNOS, whereas apoptosis were analyzed by
TUNEL method. The data exhibited with H-score and apoptotic index. The plant
extract inhibited the proliferation and survival in the breast cancer cells compared
to the stem cells. Lysimachia savranii
extract caused the increase of eNOS staining for oxidative stress and the TUNEL
labeling for apoptosis. The further molecular
techniques should be used to determine the biologic activity of Lysimachia
savranii extract and its content
should be investigated.
Primary Language | English |
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Subjects | Health Care Administration |
Journal Section | Research Article |
Authors | |
Publication Date | December 30, 2019 |
Submission Date | October 8, 2019 |
Published in Issue | Year 2019 Volume: 7 Issue: 2 |