Research Article
BibTex RIS Cite

Domates güvesinin [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının mitokondrial cytochrome oxidase subunit I (mtCOI)’e göre genetik varyasyonunun incelenmesi

Year 2016, Volume: 29 Issue: 1, 5 - 7, 01.04.2016

Abstract

Bu çalışmada Tuta absoluta`nın genetik varyasyonu mtCOI gen bölgesinin dizi analizine göre belirlenmiştir. Bu amaçla zararlının ergin bireyleri Batı Akdeniz bölgesinden Anamur, Gazipaşa, Serik, Antalya-Konyaaltı, Kumluca, Demre, Kınık ve Fethiye popülasyonlarından denemelerde kullanılmak üzere 2011 yılında domates bitkisinden toplanmıştır. Tek bir bireyden DNA izolasyonları yapılmış ve her bir popülasyondan en az 10 bireyden DNA izolasyonu yapılmıştır. DNA`lar mtCOI gen bölgesine spesifik “C1-J-2195 Forward ve TL2-N-3014 Reverse” primerleriyle çoğaltılmıştır. Sekans analizi sonunda yaklaşık olarak 747 bazlık sekans dizilimi Mega 4 programı ile dizi doğrulaması yapılarak analiz edilmiştir. Analizler sonucunda popülasyonlar arasında ve popülasyon içindeki bireyler arasında genetik farklılığın olmadığı belirlenmiştir. Gen bankasından elde edilen verilere göre Türkiye popülasyonları ile Güney Amerika ve bazı Avrupa popülasyonları arasında yüksek benzerlik olduğu gözlemlenmiştir. Sekans verileri ayrıca gen banka kaydedilmiştir.

References

  • Bettaibi A, Mezghani-Khemakhem M, Bouktila D, Makni H, Makni M, (2012) Genetic Variability of the tomato leaf miner (Tuta absoluta Meyrick; Lepidoptera: Gelechiidae), in Tunisia, inferred from RAPD-PCR. Chilean Journal of Agricultural Research 72(2) 212-216
  • Cifuentes D, Chynoweth R, Bielza P (2011) Genetic study of Mediterranean and South American populations of tomato leafminer Tuta absoluta (Povolny, 1994) (Lepidoptera:Gelechiidae) using ribosomal and mitochondrial markers. Pest Management Science 67(9): 1155-1162
  • Duric Z, Delic D, Hrncic S, Radonjic S, (2014) Distribution and molecular identification of Tuta absoluta (MEYRICK, 1917) (Lepidoptera, Gelechiidae) populations in Bosnia and Herzegovina and Montenegro. Polish journal of Entomology 83: 121-129
  • Erler F, Can M., Erdogan M, Ateş AÖ, Pradier T (2010) New Record of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) on Greenhouse-Grown Tomato in Southwestern Turkey (Antalya). Journal of Entomological Science 45(4): 392-393
  • Flores LV, Gilardon E, Gardenal CN (2003) Genetic Structure of populations of Tuta absoluta Meyrick (Lepidoptera: Gelechiidae). Journal of Basic & Applied Genetics 15(1): 29-32
  • Guillemaud T, Blin A, Le Goff I, Desneux N, Reyes M, Tabone E, Tsagkarakou A, Nino L, Lambert E (2015) The tomato borer, Tuta absoluta invading the Mediterranean Basin, originates from a single introduction from Central Chile. Scientific reports. doi: 10.1038/srep08371
  • Kılıç T (2010) First record of Tuta absoluta in Turkey. Phytoparasitica 38: 243–244
  • Kimpton CP, Gill P, Walton A, Urquhart A, Millican ES, Adams M (1993) Automated DNA profiling employing multiplex amplification of short tandem repeat loci. PCR methods and applications 3: 13-22.
  • OEPP⁄EPPO (2005) Data sheets on quarantine pests: Tuta absoluta. EPPO Bulletin 35: 434–435.
  • Proffit M, Birgersson G, Bengtsson M, Reis Jr. R, Witzgall P, Lima E (2011) Attraction and Oviposition of Tuta absoluta Females in Response to Tomato Leaf Volatiles. Journal of Chemical Ecology 37:565–574
  • Salas J. (2004) Capture of Tuta absoluta (Lepidoptera: Gelechiidae) in traps baited with its sex pheromone. The Revista Colombiana de Entomología 30: 75–78.
  • Simon C, Frati F, Bechenbach A, Crespi B, Liu H, Flook P (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequence and compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87: 651-701.
  • Suinaga FA, Casali VWD, Picanço M, Foster J (2004) Genetic divergence among tomato leafminer populations based on AFLP analysis. Pesq. Agropec. Bras. 39(7) 645-651
  • Urbaneja A, Vercher R, Navarro V, Garcı´a Marı´ F, Porcuna JL (2007) La polilla del tomate, Tuta absoluta. Phytoma Espana 194: 16–23
  • Vijayan K, Anuradha HJ, Nair CV, Pradeep AR, Awasthi AK, Saratchandra B, Rahman SAS, Singh KC, Chakraborti R, Urs SR (2006) Genetic diversity and differentiation among populations of the Indian eri silkworm, Samia cynthia ricini, revealed by ISSR markers. Journal of Insect Science 6: 1-11
  • Vos P, Hogers R, Bleeker M, Reijans M, Van De Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.
  • Yükselbaba U, Göçmen H, İkten C (2011) Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)’nın mitokondrial cytochrome oxidase subunit I (mtCOI) gen bölgesinin belirlenmesi. Türkiye IV. Bitki Koruma Kongresi 28‐30 Haziran 2011, sf. 251, Kahramanmaraş.
  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research18, 6531–6535.
  • Witzgall P, Kirsch P, Cork A (2010) Sex pheromones and their impact on pest management. Journal of Chemical Ecology 36(1): 80-100

Determination of genetic variation of tomato leafminer [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)] populations from west mediterreanean region of Turkey based on mitochondrial cytochrome oxidase I (mtCOI)

Year 2016, Volume: 29 Issue: 1, 5 - 7, 01.04.2016

Abstract

In this study, the genetic variation of Tuta absoluta was studied based on the sequences of mtCOI gene region. For this purpose, the samples of the adult pest were collected from tomato plants grown in Anamur, Gazipaşa, Serik, Antalya-Konyaaltı, Kumluca, Demre, Kınık and Fethiye district of western Mediterranean region in 2011. Minimum ten DNA was extracted individually from each. The mtCOI gene was amplified and sequenced with the primer pair “C1-J-2195 and TL2-N-3014 specific to this region. The sequences were aligned and analyzed by using Mega 4 software program. An approximately 747 base pair of informative sequences were obtained and used in the analyses. The results of the analysis showed that there was no inter and intra-population polymorphism in the sequences of individuals among the populations studied here. A high sequence similarity was found when the sequences of Turkish populations were compared with the Gen bank sequences of the South American and some European populations of the pest. Sequences were deposited in Gene bank.

References

  • Bettaibi A, Mezghani-Khemakhem M, Bouktila D, Makni H, Makni M, (2012) Genetic Variability of the tomato leaf miner (Tuta absoluta Meyrick; Lepidoptera: Gelechiidae), in Tunisia, inferred from RAPD-PCR. Chilean Journal of Agricultural Research 72(2) 212-216
  • Cifuentes D, Chynoweth R, Bielza P (2011) Genetic study of Mediterranean and South American populations of tomato leafminer Tuta absoluta (Povolny, 1994) (Lepidoptera:Gelechiidae) using ribosomal and mitochondrial markers. Pest Management Science 67(9): 1155-1162
  • Duric Z, Delic D, Hrncic S, Radonjic S, (2014) Distribution and molecular identification of Tuta absoluta (MEYRICK, 1917) (Lepidoptera, Gelechiidae) populations in Bosnia and Herzegovina and Montenegro. Polish journal of Entomology 83: 121-129
  • Erler F, Can M., Erdogan M, Ateş AÖ, Pradier T (2010) New Record of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) on Greenhouse-Grown Tomato in Southwestern Turkey (Antalya). Journal of Entomological Science 45(4): 392-393
  • Flores LV, Gilardon E, Gardenal CN (2003) Genetic Structure of populations of Tuta absoluta Meyrick (Lepidoptera: Gelechiidae). Journal of Basic & Applied Genetics 15(1): 29-32
  • Guillemaud T, Blin A, Le Goff I, Desneux N, Reyes M, Tabone E, Tsagkarakou A, Nino L, Lambert E (2015) The tomato borer, Tuta absoluta invading the Mediterranean Basin, originates from a single introduction from Central Chile. Scientific reports. doi: 10.1038/srep08371
  • Kılıç T (2010) First record of Tuta absoluta in Turkey. Phytoparasitica 38: 243–244
  • Kimpton CP, Gill P, Walton A, Urquhart A, Millican ES, Adams M (1993) Automated DNA profiling employing multiplex amplification of short tandem repeat loci. PCR methods and applications 3: 13-22.
  • OEPP⁄EPPO (2005) Data sheets on quarantine pests: Tuta absoluta. EPPO Bulletin 35: 434–435.
  • Proffit M, Birgersson G, Bengtsson M, Reis Jr. R, Witzgall P, Lima E (2011) Attraction and Oviposition of Tuta absoluta Females in Response to Tomato Leaf Volatiles. Journal of Chemical Ecology 37:565–574
  • Salas J. (2004) Capture of Tuta absoluta (Lepidoptera: Gelechiidae) in traps baited with its sex pheromone. The Revista Colombiana de Entomología 30: 75–78.
  • Simon C, Frati F, Bechenbach A, Crespi B, Liu H, Flook P (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequence and compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87: 651-701.
  • Suinaga FA, Casali VWD, Picanço M, Foster J (2004) Genetic divergence among tomato leafminer populations based on AFLP analysis. Pesq. Agropec. Bras. 39(7) 645-651
  • Urbaneja A, Vercher R, Navarro V, Garcı´a Marı´ F, Porcuna JL (2007) La polilla del tomate, Tuta absoluta. Phytoma Espana 194: 16–23
  • Vijayan K, Anuradha HJ, Nair CV, Pradeep AR, Awasthi AK, Saratchandra B, Rahman SAS, Singh KC, Chakraborti R, Urs SR (2006) Genetic diversity and differentiation among populations of the Indian eri silkworm, Samia cynthia ricini, revealed by ISSR markers. Journal of Insect Science 6: 1-11
  • Vos P, Hogers R, Bleeker M, Reijans M, Van De Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.
  • Yükselbaba U, Göçmen H, İkten C (2011) Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)’nın mitokondrial cytochrome oxidase subunit I (mtCOI) gen bölgesinin belirlenmesi. Türkiye IV. Bitki Koruma Kongresi 28‐30 Haziran 2011, sf. 251, Kahramanmaraş.
  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research18, 6531–6535.
  • Witzgall P, Kirsch P, Cork A (2010) Sex pheromones and their impact on pest management. Journal of Chemical Ecology 36(1): 80-100
There are 19 citations in total.

Details

Primary Language Turkish
Subjects Agricultural Engineering
Journal Section Makaleler
Authors

Utku Yükselbaba

Hüseyin Göçmen This is me

Publication Date April 1, 2016
Submission Date June 15, 2015
Published in Issue Year 2016 Volume: 29 Issue: 1

Cite

APA Yükselbaba, U., & Göçmen, H. (2016). Domates güvesinin [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının mitokondrial cytochrome oxidase subunit I (mtCOI)’e göre genetik varyasyonunun incelenmesi. Mediterranean Agricultural Sciences, 29(1), 5-7.
AMA Yükselbaba U, Göçmen H. Domates güvesinin [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının mitokondrial cytochrome oxidase subunit I (mtCOI)’e göre genetik varyasyonunun incelenmesi. Mediterranean Agricultural Sciences. April 2016;29(1):5-7.
Chicago Yükselbaba, Utku, and Hüseyin Göçmen. “Domates güvesinin [Tuta Absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının Mitokondrial Cytochrome Oxidase Subunit I (mtCOI)’e göre Genetik Varyasyonunun Incelenmesi”. Mediterranean Agricultural Sciences 29, no. 1 (April 2016): 5-7.
EndNote Yükselbaba U, Göçmen H (April 1, 2016) Domates güvesinin [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının mitokondrial cytochrome oxidase subunit I (mtCOI)’e göre genetik varyasyonunun incelenmesi. Mediterranean Agricultural Sciences 29 1 5–7.
IEEE U. Yükselbaba and H. Göçmen, “Domates güvesinin [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının mitokondrial cytochrome oxidase subunit I (mtCOI)’e göre genetik varyasyonunun incelenmesi”, Mediterranean Agricultural Sciences, vol. 29, no. 1, pp. 5–7, 2016.
ISNAD Yükselbaba, Utku - Göçmen, Hüseyin. “Domates güvesinin [Tuta Absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının Mitokondrial Cytochrome Oxidase Subunit I (mtCOI)’e göre Genetik Varyasyonunun Incelenmesi”. Mediterranean Agricultural Sciences 29/1 (April 2016), 5-7.
JAMA Yükselbaba U, Göçmen H. Domates güvesinin [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının mitokondrial cytochrome oxidase subunit I (mtCOI)’e göre genetik varyasyonunun incelenmesi. Mediterranean Agricultural Sciences. 2016;29:5–7.
MLA Yükselbaba, Utku and Hüseyin Göçmen. “Domates güvesinin [Tuta Absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının Mitokondrial Cytochrome Oxidase Subunit I (mtCOI)’e göre Genetik Varyasyonunun Incelenmesi”. Mediterranean Agricultural Sciences, vol. 29, no. 1, 2016, pp. 5-7.
Vancouver Yükselbaba U, Göçmen H. Domates güvesinin [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)] Batı Akdeniz Bölgesi populasyonlarının mitokondrial cytochrome oxidase subunit I (mtCOI)’e göre genetik varyasyonunun incelenmesi. Mediterranean Agricultural Sciences. 2016;29(1):5-7.

Creative Commons License

Mediterranean Agricultural Sciences is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.