Research Article
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Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi

Year 2020, Volume: 27 Issue: 3, 273 - 282, 01.09.2020
https://doi.org/10.17343/sdutfd.554038

Abstract

Bu çalışmada, önemli bir çevre
kirletici ve dokular üzerine zararlı etkileri olan kadmiyumun erkek fertilitesi
üzerine oluşturduğu hasara karşı resveratrolün ve dialil disülfitin koruyucu
etkileri araştırıldı.



Yapılan deneyde Wistar albino cinsi
rat kullanıldı ve 5 grup (A: kontrol, B1: CdCl2, B2: RES + CdCl2,
B3: DDS + CdCl2, B4: RES + DDS + CdCl2) oluşturuldu.
Histolojik değişiklikleri değerlendirmek için testis dokuları, Hematoksilen ve
Eozin ile boyandı ve Modifiye Johnsen skorlaması yapıldı. Gruplarda gözlenen
apoptoz, TUNEL yöntemi ve aktif kaspaz-3 immunohistokimyası ile
değerlendirildi.



Elde ettiğimiz bulgulara göre, B1
grubunda, kadmiyumun seminifer tübüllerde hasara yol açtığı, spermatogenezi
durdurduğu ve germ hürcelerinde apoptozu indüklediği gözlendi. Modifiye Johnsen
skorlama sonuçlarında kontrole göre B1 grubunda anlamlı derecede azalma,
apoptotik indeks sonuçlarında ise anlamlı derecede artış görüldü. B1 grubuyla
B2, B3 ve B4 grupları karşılaştırıldığında; Modifiye Johnsen skorlama
sonuçlarında B1 ile B2 grubu arasında anlamlı bir farklılık görülmezken, B3 ve
B4 gruplarında anlamlı artış görüldü. Ayrıca apoptotik indeks sonuçlarında B2
grubunda anlamlı bir farklılık görülmezken, B3 ve B4 gruplarında anlamlı azalma
görüldü.



Sonuç olarak, sıçan testisinde
kadmiyum ile oluşturulan akut hasarın baskılanmasında resveratrol ve dialil
disülfitin rolüne yönelik bilgiler ortaya konuldu. Belirlediğimiz dozda
resveratrol bu akut hasarın baskılanmasında etkili olmazken dialil disülfit ve
resveratrol ile birlikte kullanımı hasarı önlemede etkili bulundu. Literatür
analizimize göre bu çalışma kadmiyumun indüklediği testis hasarına karşı dialil
disülfitin koruyucu etkilerinin gösterildiği ilk çalışmadır.

References

  • ATSDR. Toxicological profile for cadmium. J Public Heal. Agency for Toxic Substances and Disease Registry (US), 2012;1–487.
  • WHO. Air quality guidelines for Europe. Environmental Science and Pollution Research. 2nd edition. WHO Regional Publications, Copenhagen, 2000;pp:136-139.
  • Waalkes MP. Cadmium carcinogenesis. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2003;533: 107-120.
  • Kara H, Karataş F, Canatan H. Effect of single dose cadmium chloride administration on oxidative stress in male and female rats. Turkısh J Veterınary Anımal Scıences. 2005;29:37–42.
  • Koca N, Karadeniz F. Gıdalardaki doğal antioksidan bileşikler. Gıda Derg. 2005;30(4):229–36.
  • Lee KM, Lee IC, Kim SH ve ark. Melatonin attenuates doxorubicin-induced testicular toxicity in rats. Andrologia. 2012;44:796–803.
  • Sergides C, Chirilă M, Silvestro L, Pitta D, Pittas A. Bioavailability and safety study of resveratrol 500 mg tablets in healthy male and female volunteers. Exp Ther Med. 2016;11(1):164–70.
  • Aires V, Limagne E, Cotte AK, Latruffe N, Ghiringhelli F, Delmas D. Resveratrol metabolites inhibit human metastatic colon cancer cells progression and synergize with chemotherapeutic drugs to induce cell death. Mol Nutr Food Res. 2013;57(7):1170–81.
  • Bishayee A, Darvesh AS, Politis T, McGory R. Resveratrol and liver disease: Frombench to bedside and community. Liver Int. 2010;30(8):1103–14.
  • Kim SH, Lee IC, Baek H-S, Moon C, Kim S-H, Kim J-C. Protective effect of diallyl disulfide on cyclophosphamide-induced testicular toxicity in rats. Lab Anim Res 2013;29(4):204–11.
  • Fukao T, Hosono T, Misawa S, Seki T, Ariga T. The effects of allyl sulfides on the induction of phase II detoxification enzymes and liver injury by carbon tetrachloride. Food Chem Toxicol. 2004;42(5):743–9.
  • Guyonnet D, Belloir C, Suschetet M, Siess M-H, Le Bon A-M. Mechanisms of protection against aflatoxin B1 genotoxicity in rats treated by organosulfur compounds from garlic. Carcinogenesis. 2002;23(8):1335–41.
  • Nakagawa H, Tsuta K, Kiuchi K ve ark. Growth inhibitory effects of diallyl disulfide on human breast cancer cell lines. Carcinogenesis. 2001;22(6):891–7.
  • Pedraza-Chaverrí J, González-Orozco AE, Maldonado PD, Barrera D, Medina-Campos ON, Hernández-Pando R. Diallyl disulfide ameliorates gentamicin-induced oxidative stress and nephropathy in rats. Eur J Pharmacol. 2003;473(1):71–8.
  • Pari L, Murugavel P, Sitasawad SL, Kumar KS. Cytoprotective and antioxidant role of diallyl tetrasulfide on cadmium induced renal injury: An in vivo and in vitro study. Life Sci. 2007;80(7):650–8.
  • Palsamy P, Subramanian S. Resveratrol protects diabetic kidney by attenuating hyperglycemia-mediated oxidative stress and renal inflammatory cytokines via Nrf2-Keap1 signaling. Biochim Biophys Acta - Mol Basis Dis. 2011;1812(7):719–31.
  • Di C, Han L, Zhang H ve ark. Diallyl disulfide attenuated carbon ion irradiation-induced apoptosis in mouse testis through changing the ratio of Tap73/ Δ Np73 via mitochondrial pathway. Nat Publ Gr. Nature Publishing Group; 2015;86(931):1–11.
  • Erdemir F, Atilgan D, Markoc F, Boztepe O, Suha-Parlaktas B, Sahin S. The effect of diet induced obesity on testicular tissue and serum oxidative stress parameters. Actas Urol españolas. 2012;36(3):153–9.
  • Odacı E, Hancı H, Yuluğ E ve ark. Effects of prenatal exposure to a 900 MHz electromagnetic field on 60-day-old rat testis and epididymal sperm quality. Biotech Histochem. 2016;91(1):9–19.
  • Damek-Poprawa M, Sawicka-Kapusta K. Histopathological changes in the liver, kidneys, and testes of bank voles environmentally exposed to heavy metal emissions from the steelworks and zinc smelter in Poland. Environ Res. 2004;96(1):72–8.
  • Klaassen CD, Liu J, Diwan BA. Metallothionein Protection of Cadmium Toxicity. Toxicol Appl Pharmacol. 2009;238(3):215–20.
  • Siu ER, Mruk DD, Porto CS, Cheng CY. Cadmium-induced testicular injury. Toxicology and Applied Pharmacology. 2010;238:240–9.
  • De Souza Predes F, Diamante MAS, Dolder H. Testis response to low doses of cadmium in Wistar rats. Int J Exp Pathol. 2010;91(2):125–31.
  • Herranz LM, Teba F, Martín R ve ark. Quantitative changes in rat seminiferous epithelium after chronic administration of low doses of cadmium and zinc : A Stereological Study. The Open Andrology Journal. 2010;27–36.
  • Othman MS, Nada A, Zaki HS, Abdel Moneim AE. Effect of Physalis peruviana L. on cadmium-induced testicular toxicity in rats. Biol Trace Elem Res. 2014;159(1–3):278–87.
  • Kerr JB. Spontaneous degeneration of germ cells in normal rat testis: assessment of cell types and frequency during the spermatogenic cycle. J Reprod Fertil. 1992;95(3):825–30.
  • Athar M, Back JH, Tang X ve ark. Resveratrol: a review of pre-clinical studies for human cancer prevention. Toxicol Appl Pharmacol. 2007;224(3):274–83.
  • Olas B, Nowak P, Kolodziejczyk J, Ponczek M, Wachowicz B. Protective effects of resveratrol against oxidative/nitrative modifications of plasma proteins and lipids exposed to peroxynitrite. J Nutr Biochem. 2006;17(2):96–102.
  • Patel KR, Brown VA, Jones DJL ve ark. Clinical pharmacology of resveratrol and its metabolites in colorectal cancer patients. Cancer Res. 2010;70(19):7392–9.
  • Hoshino J, Park E-J, Kondratyuk TP ve ark. Selective synthesis and biological evaluation of sulfate- conjugated resveratrol metabolites. J Med Chem. 2011;53(13):5033–43.
  • Bowers JL, Tyulmenkov V V, Jernigan SC, Klinge CM. Resveratrol acts as a mixed agonist/antagonist for estrogen receptors alfa and beta. Endocrinology. 2000;141(10):3657–67.
  • Wallerath T, Deckert G, Ternes T ve ark. Resveratrol, a polyphenolic phytoalexin present in red wine, enhances expression and activity of endothelial nitric oxide synthase. Circulation. 2002;106(13):1652–8.
  • Chen CK, Pace-Asciak CR. Vasorelaxing activity of resveratrol and quercetin in isolated rat aorta. Gen Pharmacol. 1996;27(2):363–6.
  • Orallo F, Alvarez E, Camiña M, Leiro JM, Gómez E, Fernández P. The possible implication of trans-resveratrol in the cardioprotective effects of long-term moderate wine consumption. Mol Pharmacol. 2002;61(2):294–302.
  • Rakici O, Kiziltepe U, Coskun B, Aslamaci S, Akar F. Effects of resveratrol on vascular tone and endothelial function of human saphenous vein and internal mammary artery. Int J Cardiol. 2005;105(2):209–15.
  • Hussein MMA, Mahfouz MK. Effect of resveratrol and rosuvastatin on experimental diabetic nephropathy in rats. Biomed Pharmacother. 2016;82:685–92.
  • Li E, Guo Y, Wang G, Chen F, Li Q. Effect of resveratrol on restoring spermatogenesis in experimental cryptorchid mice and analysis of related differentially expressed proteins. Cell Biol Int. 2015;39(6):733–40.
  • Palsamy P, Sivakumar S, Subramanian S. Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin–nicotinamide-induced experimental diabetic rats. Chem Biol Interact. 2010;186(2):200–10.
  • Silan C. The effects of chronic resveratrol treatment on vascular responsiveness of streptozotocin-induced diabetic rats. Biol Pharm Bull. 2008;31(5):897–902.
  • Ranawat P, Khanduja KL, Pathak CM. Resveratrol - an ingredient of red wine abrogates the reproductive capacity in male mice. Andrologia. 2014;46(6):650–8.
  • Gehm BD, Mcandrews JM, Chien P-Y, Jameson JL. Resveratrol, a polyphenolic compound found in grapes and wine, is an agonist for the estrogen receptor. Physiology. 1997;94:14138–43.
  • Lu R, Serrero G. Resveratrol, a natural product derived from grape, exhibits antiestrogenic activity and inhibits the growth of human breast cancer cells. Journal of Cellular Physiology. 1999; 179:297–304.
  • Svechnikov K, Izzo G, Landreh L, Weisser J, Sder O. Endocrine disruptors and leydig cell function. Journal of Biomedicine and Biotechnology. 2010;2010:10 pages.
  • Takiguchi M, Yoshihara S. New aspects of cadmium as endocrine disruptor. Environ Sci. 2006;13(2):107–16.
  • Le Corre L, Chalabi N, Delort L, Bignon Y-J, Bernard-Gallon DJ. Resveratrol and breast cancer chemoprevention: molecular mechanisms. Mol Nutr Food Res. 2005;49(5):462–71.
  • Siu ER, Wong EWP, Mruk DD, Sze KL, Porto CS, Cheng CY. An occludin-focal adhesion kinase protein complex at the blood-testis barrier: A study using the cadmium model. Endocrinology. 2009;150(7):3336–44.
  • Chung NPY, Cheng CY. Is cadmium chloride-induced inter-Sertoli tight junction permeability barrier disruption a suitable in vitro model to study the events of junction disassembly during spermatogenesis in the rat testis? Endocrinology. 2001;142(5):1878–88.
  • Lee IC, Baek HS, Kim SH ve ark. Effect of diallyl disulfide on acute gastric mucosal damage induced by alcohol in rats. Hum Exp Toxicol. 2014;1–13.
Year 2020, Volume: 27 Issue: 3, 273 - 282, 01.09.2020
https://doi.org/10.17343/sdutfd.554038

Abstract

References

  • ATSDR. Toxicological profile for cadmium. J Public Heal. Agency for Toxic Substances and Disease Registry (US), 2012;1–487.
  • WHO. Air quality guidelines for Europe. Environmental Science and Pollution Research. 2nd edition. WHO Regional Publications, Copenhagen, 2000;pp:136-139.
  • Waalkes MP. Cadmium carcinogenesis. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2003;533: 107-120.
  • Kara H, Karataş F, Canatan H. Effect of single dose cadmium chloride administration on oxidative stress in male and female rats. Turkısh J Veterınary Anımal Scıences. 2005;29:37–42.
  • Koca N, Karadeniz F. Gıdalardaki doğal antioksidan bileşikler. Gıda Derg. 2005;30(4):229–36.
  • Lee KM, Lee IC, Kim SH ve ark. Melatonin attenuates doxorubicin-induced testicular toxicity in rats. Andrologia. 2012;44:796–803.
  • Sergides C, Chirilă M, Silvestro L, Pitta D, Pittas A. Bioavailability and safety study of resveratrol 500 mg tablets in healthy male and female volunteers. Exp Ther Med. 2016;11(1):164–70.
  • Aires V, Limagne E, Cotte AK, Latruffe N, Ghiringhelli F, Delmas D. Resveratrol metabolites inhibit human metastatic colon cancer cells progression and synergize with chemotherapeutic drugs to induce cell death. Mol Nutr Food Res. 2013;57(7):1170–81.
  • Bishayee A, Darvesh AS, Politis T, McGory R. Resveratrol and liver disease: Frombench to bedside and community. Liver Int. 2010;30(8):1103–14.
  • Kim SH, Lee IC, Baek H-S, Moon C, Kim S-H, Kim J-C. Protective effect of diallyl disulfide on cyclophosphamide-induced testicular toxicity in rats. Lab Anim Res 2013;29(4):204–11.
  • Fukao T, Hosono T, Misawa S, Seki T, Ariga T. The effects of allyl sulfides on the induction of phase II detoxification enzymes and liver injury by carbon tetrachloride. Food Chem Toxicol. 2004;42(5):743–9.
  • Guyonnet D, Belloir C, Suschetet M, Siess M-H, Le Bon A-M. Mechanisms of protection against aflatoxin B1 genotoxicity in rats treated by organosulfur compounds from garlic. Carcinogenesis. 2002;23(8):1335–41.
  • Nakagawa H, Tsuta K, Kiuchi K ve ark. Growth inhibitory effects of diallyl disulfide on human breast cancer cell lines. Carcinogenesis. 2001;22(6):891–7.
  • Pedraza-Chaverrí J, González-Orozco AE, Maldonado PD, Barrera D, Medina-Campos ON, Hernández-Pando R. Diallyl disulfide ameliorates gentamicin-induced oxidative stress and nephropathy in rats. Eur J Pharmacol. 2003;473(1):71–8.
  • Pari L, Murugavel P, Sitasawad SL, Kumar KS. Cytoprotective and antioxidant role of diallyl tetrasulfide on cadmium induced renal injury: An in vivo and in vitro study. Life Sci. 2007;80(7):650–8.
  • Palsamy P, Subramanian S. Resveratrol protects diabetic kidney by attenuating hyperglycemia-mediated oxidative stress and renal inflammatory cytokines via Nrf2-Keap1 signaling. Biochim Biophys Acta - Mol Basis Dis. 2011;1812(7):719–31.
  • Di C, Han L, Zhang H ve ark. Diallyl disulfide attenuated carbon ion irradiation-induced apoptosis in mouse testis through changing the ratio of Tap73/ Δ Np73 via mitochondrial pathway. Nat Publ Gr. Nature Publishing Group; 2015;86(931):1–11.
  • Erdemir F, Atilgan D, Markoc F, Boztepe O, Suha-Parlaktas B, Sahin S. The effect of diet induced obesity on testicular tissue and serum oxidative stress parameters. Actas Urol españolas. 2012;36(3):153–9.
  • Odacı E, Hancı H, Yuluğ E ve ark. Effects of prenatal exposure to a 900 MHz electromagnetic field on 60-day-old rat testis and epididymal sperm quality. Biotech Histochem. 2016;91(1):9–19.
  • Damek-Poprawa M, Sawicka-Kapusta K. Histopathological changes in the liver, kidneys, and testes of bank voles environmentally exposed to heavy metal emissions from the steelworks and zinc smelter in Poland. Environ Res. 2004;96(1):72–8.
  • Klaassen CD, Liu J, Diwan BA. Metallothionein Protection of Cadmium Toxicity. Toxicol Appl Pharmacol. 2009;238(3):215–20.
  • Siu ER, Mruk DD, Porto CS, Cheng CY. Cadmium-induced testicular injury. Toxicology and Applied Pharmacology. 2010;238:240–9.
  • De Souza Predes F, Diamante MAS, Dolder H. Testis response to low doses of cadmium in Wistar rats. Int J Exp Pathol. 2010;91(2):125–31.
  • Herranz LM, Teba F, Martín R ve ark. Quantitative changes in rat seminiferous epithelium after chronic administration of low doses of cadmium and zinc : A Stereological Study. The Open Andrology Journal. 2010;27–36.
  • Othman MS, Nada A, Zaki HS, Abdel Moneim AE. Effect of Physalis peruviana L. on cadmium-induced testicular toxicity in rats. Biol Trace Elem Res. 2014;159(1–3):278–87.
  • Kerr JB. Spontaneous degeneration of germ cells in normal rat testis: assessment of cell types and frequency during the spermatogenic cycle. J Reprod Fertil. 1992;95(3):825–30.
  • Athar M, Back JH, Tang X ve ark. Resveratrol: a review of pre-clinical studies for human cancer prevention. Toxicol Appl Pharmacol. 2007;224(3):274–83.
  • Olas B, Nowak P, Kolodziejczyk J, Ponczek M, Wachowicz B. Protective effects of resveratrol against oxidative/nitrative modifications of plasma proteins and lipids exposed to peroxynitrite. J Nutr Biochem. 2006;17(2):96–102.
  • Patel KR, Brown VA, Jones DJL ve ark. Clinical pharmacology of resveratrol and its metabolites in colorectal cancer patients. Cancer Res. 2010;70(19):7392–9.
  • Hoshino J, Park E-J, Kondratyuk TP ve ark. Selective synthesis and biological evaluation of sulfate- conjugated resveratrol metabolites. J Med Chem. 2011;53(13):5033–43.
  • Bowers JL, Tyulmenkov V V, Jernigan SC, Klinge CM. Resveratrol acts as a mixed agonist/antagonist for estrogen receptors alfa and beta. Endocrinology. 2000;141(10):3657–67.
  • Wallerath T, Deckert G, Ternes T ve ark. Resveratrol, a polyphenolic phytoalexin present in red wine, enhances expression and activity of endothelial nitric oxide synthase. Circulation. 2002;106(13):1652–8.
  • Chen CK, Pace-Asciak CR. Vasorelaxing activity of resveratrol and quercetin in isolated rat aorta. Gen Pharmacol. 1996;27(2):363–6.
  • Orallo F, Alvarez E, Camiña M, Leiro JM, Gómez E, Fernández P. The possible implication of trans-resveratrol in the cardioprotective effects of long-term moderate wine consumption. Mol Pharmacol. 2002;61(2):294–302.
  • Rakici O, Kiziltepe U, Coskun B, Aslamaci S, Akar F. Effects of resveratrol on vascular tone and endothelial function of human saphenous vein and internal mammary artery. Int J Cardiol. 2005;105(2):209–15.
  • Hussein MMA, Mahfouz MK. Effect of resveratrol and rosuvastatin on experimental diabetic nephropathy in rats. Biomed Pharmacother. 2016;82:685–92.
  • Li E, Guo Y, Wang G, Chen F, Li Q. Effect of resveratrol on restoring spermatogenesis in experimental cryptorchid mice and analysis of related differentially expressed proteins. Cell Biol Int. 2015;39(6):733–40.
  • Palsamy P, Sivakumar S, Subramanian S. Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin–nicotinamide-induced experimental diabetic rats. Chem Biol Interact. 2010;186(2):200–10.
  • Silan C. The effects of chronic resveratrol treatment on vascular responsiveness of streptozotocin-induced diabetic rats. Biol Pharm Bull. 2008;31(5):897–902.
  • Ranawat P, Khanduja KL, Pathak CM. Resveratrol - an ingredient of red wine abrogates the reproductive capacity in male mice. Andrologia. 2014;46(6):650–8.
  • Gehm BD, Mcandrews JM, Chien P-Y, Jameson JL. Resveratrol, a polyphenolic compound found in grapes and wine, is an agonist for the estrogen receptor. Physiology. 1997;94:14138–43.
  • Lu R, Serrero G. Resveratrol, a natural product derived from grape, exhibits antiestrogenic activity and inhibits the growth of human breast cancer cells. Journal of Cellular Physiology. 1999; 179:297–304.
  • Svechnikov K, Izzo G, Landreh L, Weisser J, Sder O. Endocrine disruptors and leydig cell function. Journal of Biomedicine and Biotechnology. 2010;2010:10 pages.
  • Takiguchi M, Yoshihara S. New aspects of cadmium as endocrine disruptor. Environ Sci. 2006;13(2):107–16.
  • Le Corre L, Chalabi N, Delort L, Bignon Y-J, Bernard-Gallon DJ. Resveratrol and breast cancer chemoprevention: molecular mechanisms. Mol Nutr Food Res. 2005;49(5):462–71.
  • Siu ER, Wong EWP, Mruk DD, Sze KL, Porto CS, Cheng CY. An occludin-focal adhesion kinase protein complex at the blood-testis barrier: A study using the cadmium model. Endocrinology. 2009;150(7):3336–44.
  • Chung NPY, Cheng CY. Is cadmium chloride-induced inter-Sertoli tight junction permeability barrier disruption a suitable in vitro model to study the events of junction disassembly during spermatogenesis in the rat testis? Endocrinology. 2001;142(5):1878–88.
  • Lee IC, Baek HS, Kim SH ve ark. Effect of diallyl disulfide on acute gastric mucosal damage induced by alcohol in rats. Hum Exp Toxicol. 2014;1–13.
There are 48 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences
Journal Section Research Articles
Authors

Kıymet Halk

Zeynep Kahveci This is me

İlkin Çavuşoğlu

Şahin Sırmalı This is me

Publication Date September 1, 2020
Submission Date May 2, 2019
Acceptance Date June 28, 2019
Published in Issue Year 2020 Volume: 27 Issue: 3

Cite

Vancouver Halk K, Kahveci Z, Çavuşoğlu İ, Sırmalı Ş. Resveratrol ve Dialil Disülfitin Sıçan Testisinde Kadmiyum ile Oluşturulmuş Akut Hasara Etkisi. Med J SDU. 2020;27(3):273-82.

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