Ksantin Biyosensörlerin Performansına Çeşitli Çok Duvarlı Karbon Nanotüplerin Etkisi

Yıl 2018, Cilt: 22 Sayı: 2, 832 - 839, 15.08.2018

Ceren Kaçar , Berna Dalkıran , Pınar Esra Erden , Esma Kılıç

Öz

Bu çalışmada, fonksiyonel grup içermeyen (MWCNT) ve karboksil (COOH-MWCNT), hidroksil (OH-MWCNT) ve amino (NH₂-MWCNT) grubu içeren çok duvarlı karbon nanotüplerin amperometrik ksantin biyosensörünün cevabına etkileri incelendi. Bu amaçla, MWCNT’ler ve ksantin oksidaz (XO) enzimi jelatin çözeltisi içinde dağıtıldı ve bu karışımlardan belirli miktarların camsı karbon elektrot yüzeyine damlatılması ile ksantin biyosensörleri hazırlandı. Bu biyosensörlerin optimum pH, enzim miktarı gibi çalışma koşulları belirlendi ve bu koşullarda biyosensörlerin performans faktörleri incelendi. NH₂-MWCNT ile hazırlanan biyosensörün en yüksek duyarlık ile ksantine cevap verdiği gözlendi. Bu biyosensör ksantine <15 s gibi kısa bir cevap süresi ile 9,9×10^-7 M – 9,4×10^-4 M aralığında doğrusal cevap gösterdi ve gözlenebilme sınırı 8,7×10^-7 M, duyarlığı 23,36 µA mM^-1 olarak belirlendi.

Anahtar Kelimeler

Biyosensör, Amperometri; Çok duvarlı karbon nanotüp; Ksantin; Ksantin oksidaz

Kaynakça

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