Araştırma Makalesi
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SÜT TOZU VE PEYNİRALTI SUYU TOZUNDA ANTİBİYOTİK KALINTILARININ UPLC-MS/MS İLE ANALİZİNDE ÇOKLU GRUP YÖNTEMİ

Yıl 2022, Cilt: 47 Sayı: 1, 25 - 33, 23.12.2021
https://doi.org/10.15237/gida.GD21124

Öz

Gıda endüstrisinde birçok ürünün hammaddesi olarak yaygın bir şekilde kullanılan süt tozu ve peyniraltı suyu tozunda beş gruptan (makrolidler, sülfanamidler, kinolonlar, linkozamidler, diaminopirimidinler) 27 antibiyotik kalıntısının belirlenmesi için bir ultra-performans sıvı kromatografisi-tandem kütle spektrometrisi (UPLC-MS/MS) çok sınıflı bir yöntem geliştirilmiş ve valide edilmiştir. Valide edilmiş bu yöntemin ana avantajı, metanol ile numunelerin ekstraksiyonuna dayanan basit, ucuz ve hızlı numune hazırlamadır. Yöntemin validasyonu, Avrupa ve Türkiye'deki resmi denetim numunelerinin analizinde kullanılan yöntemler için gerekli prosedür olan 2002/657/EC sayılı Avrupa Komisyonu Kararında belirtilen gereklilikler izlenerek gerçekleştirilmiştir. Matriks uyumlu kalibrasyon kullanılarak, numune hazırlama ve enstrümantal analiz ile ilgili matriks etkisi ve belirsizlikler minimize edilmiştir. Yöntem, kalıntıları çok düşük konsantrasyonlarda tespit etme yeteneğine sahiptir.

Destekleyen Kurum

Gıda ve Yem Kontrol Merkez Araştırma Enstitüsü

Kaynakça

  • Beyene, T. (2016). Veterinary drug residues in food-animal products: its risk factors and potential effects on public health. J Vet Sci Technol, 7(1), 1-7.
  • Barbut, S. (2006). Effects of caseinate, whey and milk powders on the texture and microstructure of emulsified chicken meat batters. LWT-Food Science and Technology, 39(6), 660-664.
  • Blasco, C., Picó, Y., & Torres, C. M. (2007). Progress in analysis of residual antibacterials in food. TrAC Trends in Analytical Chemistry, 26(9), 895-913.
  • Cheng, G., Hao, H., Xie, S., Wang, X., Dai, M., Huang, L., & Yuan, Z. (2014). Antibiotic alternatives: the substitution of antibiotics in animal husbandry?. Frontiers in microbiology, 5, 217. Coronado, S. A., Trout, G. R., Dunshea, F. R., & Shah, N. P. (2002). Antioxidant effects of rosemary extract and whey powder on the oxidative stability of wiener sausages during 10 months frozen storage. Meat Science, 62(2), 217-224.
  • Dasenaki, M. E., & Thomaidis, N. S. (2015). Multi-residue determination of 115 veterinary drugs and pharmaceutical residues in milk powder, butter, fish tissue and eggs using liquid chromatography–tandem mass spectrometry. Analytica Chimica Acta, 880, 103-121
  • EC (European Commission) 2002. “Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC”, Official Journal of the European Community, L 221:8-36
  • EC, 2005. Ban on antibiotics as growth promoters in animal feed enters into effect. https://ec.europa.eu/commission/presscorner/detail/en/IP_05_1687 Erişim Tarihi:25.05.2020
  • EC 2010. 37/2010/EC, on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin, Off. J. Eur. Commun. L15 (2010) 1–72.
  • González-Martınez, C., Becerra, M., Cháfer, M., Albors, A., Carot, J. M., & Chiralt, A. (2002). Influence of substituting milk powder for whey powder on yoghurt quality. Trends in Food Science & Technology, 13(9-10), 334-340
  • Hoppe, C., Andersen, G. S., Jacobsen, S., Mølgaard, C., Friis, H., Sangild, P. T., & Michaelsen, K. F. (2008). The use of whey or skimmed milk powder in fortified blended foods for vulnerable groups. The Journal of nutrition, 138(1), 145S-161S.
  • Kaufmann, A. (2009). Validation of multiresidue methods for veterinary drug residues; related problems and possible solutions. Analytica chimica acta, 637(1-2), 144-155.
  • Liang, B., & Hartel, R. W. (2004). Effects of milk powders in milk chocolate. Journal of Dairy Science, 87(1), 20-31.
  • Ministry of Agriculture and Rural Affairs, 2006. Communique Amending the Communique on the Production, Import, Export, Sale and Use of Feed Additives and Premixes (Tebliğ No: 2006/1) (RG:21.01.2006 Sayı:2656)
  • Morettı S., Salutı G. and Galarını, R., 2017. Residue determination in honey. In: Honey analysis, De Toledo V. A. (eds.), IntechOpen p. 325-365
  • Ramos, Ó. L., Pereira, R. N. C., Rodrigues, R. M. M., Teixeira, J. A., Vicente, A. A., & Malcata, F. X. (2015). Whey and whey powders: Production and uses.
  • Rezende, D. R., Filho, N. F., & Rocha, G. L. (2012). Simultaneous determination of chloramphenicol and florfenicol in liquid milk, milk powder and bovine muscle by LC–MS/MS. Food Additives & Contaminants: Part A, 29(4), 559-570
  • Secchi, N., Stara, G., Anedda, R., Campus, M., Piga, A., Roggio, T., & Catzeddu, P. (2011). Effectiveness of sweet ovine whey powder in increasing the shelf life of Amaretti cookies. LWT-Food Science and Technology, 44(4), 1073-1078.
  • Serdaroğlu, M. (2006). Improving low fat meatball characteristics by adding whey powder. Meat Science, 72(1), 155-163.
  • Tian, H., Wang, J., Zhang, Y., Li, S., Jiang, J., Tao, D., & Zheng, N. (2016). Quantitative multiresidue analysis of antibiotics in milk and milk powder by ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometry. Journal of chromatography B, 1033, 172-179.
  • Yu, C., & Hu, B. (2012). C18-coated stir bar sorptive extraction combined with high performance liquid chromatography–electrospray tandem mass spectrometry for the analysis of sulfonamides in milk and milk powder. Talanta, 90, 77-84.

A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS

Yıl 2022, Cilt: 47 Sayı: 1, 25 - 33, 23.12.2021
https://doi.org/10.15237/gida.GD21124

Öz

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) multi-class method was developed and validated for the determination of 27 antibiotic residues from five classes (macrolides, sulfonamids, quinolones, lincosamids, diaminopyrimidines) in milk powder and whey powder which are commonly used as raw material of many products in the food industry. A simple, inexpensive and fast sample preparation based on the extraction of samples with methanol is the main advantage of the validated method. The validation of the method was performed following the requirements outlined in the European Commission Decision 2002/657/EC, which is required procedure for the methods used in analysis of official audits samples in Europe and Turkey. Matrix effect and uncertainties related with sample preparation and instrumental analysis were minimised by using matrix-matched calibration. The method is capable of detecting residues at very low concentrations.

Kaynakça

  • Beyene, T. (2016). Veterinary drug residues in food-animal products: its risk factors and potential effects on public health. J Vet Sci Technol, 7(1), 1-7.
  • Barbut, S. (2006). Effects of caseinate, whey and milk powders on the texture and microstructure of emulsified chicken meat batters. LWT-Food Science and Technology, 39(6), 660-664.
  • Blasco, C., Picó, Y., & Torres, C. M. (2007). Progress in analysis of residual antibacterials in food. TrAC Trends in Analytical Chemistry, 26(9), 895-913.
  • Cheng, G., Hao, H., Xie, S., Wang, X., Dai, M., Huang, L., & Yuan, Z. (2014). Antibiotic alternatives: the substitution of antibiotics in animal husbandry?. Frontiers in microbiology, 5, 217. Coronado, S. A., Trout, G. R., Dunshea, F. R., & Shah, N. P. (2002). Antioxidant effects of rosemary extract and whey powder on the oxidative stability of wiener sausages during 10 months frozen storage. Meat Science, 62(2), 217-224.
  • Dasenaki, M. E., & Thomaidis, N. S. (2015). Multi-residue determination of 115 veterinary drugs and pharmaceutical residues in milk powder, butter, fish tissue and eggs using liquid chromatography–tandem mass spectrometry. Analytica Chimica Acta, 880, 103-121
  • EC (European Commission) 2002. “Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC”, Official Journal of the European Community, L 221:8-36
  • EC, 2005. Ban on antibiotics as growth promoters in animal feed enters into effect. https://ec.europa.eu/commission/presscorner/detail/en/IP_05_1687 Erişim Tarihi:25.05.2020
  • EC 2010. 37/2010/EC, on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin, Off. J. Eur. Commun. L15 (2010) 1–72.
  • González-Martınez, C., Becerra, M., Cháfer, M., Albors, A., Carot, J. M., & Chiralt, A. (2002). Influence of substituting milk powder for whey powder on yoghurt quality. Trends in Food Science & Technology, 13(9-10), 334-340
  • Hoppe, C., Andersen, G. S., Jacobsen, S., Mølgaard, C., Friis, H., Sangild, P. T., & Michaelsen, K. F. (2008). The use of whey or skimmed milk powder in fortified blended foods for vulnerable groups. The Journal of nutrition, 138(1), 145S-161S.
  • Kaufmann, A. (2009). Validation of multiresidue methods for veterinary drug residues; related problems and possible solutions. Analytica chimica acta, 637(1-2), 144-155.
  • Liang, B., & Hartel, R. W. (2004). Effects of milk powders in milk chocolate. Journal of Dairy Science, 87(1), 20-31.
  • Ministry of Agriculture and Rural Affairs, 2006. Communique Amending the Communique on the Production, Import, Export, Sale and Use of Feed Additives and Premixes (Tebliğ No: 2006/1) (RG:21.01.2006 Sayı:2656)
  • Morettı S., Salutı G. and Galarını, R., 2017. Residue determination in honey. In: Honey analysis, De Toledo V. A. (eds.), IntechOpen p. 325-365
  • Ramos, Ó. L., Pereira, R. N. C., Rodrigues, R. M. M., Teixeira, J. A., Vicente, A. A., & Malcata, F. X. (2015). Whey and whey powders: Production and uses.
  • Rezende, D. R., Filho, N. F., & Rocha, G. L. (2012). Simultaneous determination of chloramphenicol and florfenicol in liquid milk, milk powder and bovine muscle by LC–MS/MS. Food Additives & Contaminants: Part A, 29(4), 559-570
  • Secchi, N., Stara, G., Anedda, R., Campus, M., Piga, A., Roggio, T., & Catzeddu, P. (2011). Effectiveness of sweet ovine whey powder in increasing the shelf life of Amaretti cookies. LWT-Food Science and Technology, 44(4), 1073-1078.
  • Serdaroğlu, M. (2006). Improving low fat meatball characteristics by adding whey powder. Meat Science, 72(1), 155-163.
  • Tian, H., Wang, J., Zhang, Y., Li, S., Jiang, J., Tao, D., & Zheng, N. (2016). Quantitative multiresidue analysis of antibiotics in milk and milk powder by ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometry. Journal of chromatography B, 1033, 172-179.
  • Yu, C., & Hu, B. (2012). C18-coated stir bar sorptive extraction combined with high performance liquid chromatography–electrospray tandem mass spectrometry for the analysis of sulfonamides in milk and milk powder. Talanta, 90, 77-84.
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Gıda Mühendisliği
Bölüm Makaleler
Yazarlar

Arzu Yavuz 0000-0002-2526-4761

İsmail Azar Bu kişi benim 0000-0003-4424-208X

Ali Özcan Bu kişi benim 0000-0002-1338-7852

Niyazi Ülkü Bu kişi benim 0000-0003-3139-8827

Yayımlanma Tarihi 23 Aralık 2021
Yayımlandığı Sayı Yıl 2022 Cilt: 47 Sayı: 1

Kaynak Göster

APA Yavuz, A., Azar, İ., Özcan, A., Ülkü, N. (2021). A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS. Gıda, 47(1), 25-33. https://doi.org/10.15237/gida.GD21124
AMA Yavuz A, Azar İ, Özcan A, Ülkü N. A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS. GIDA. Aralık 2021;47(1):25-33. doi:10.15237/gida.GD21124
Chicago Yavuz, Arzu, İsmail Azar, Ali Özcan, ve Niyazi Ülkü. “A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS”. Gıda 47, sy. 1 (Aralık 2021): 25-33. https://doi.org/10.15237/gida.GD21124.
EndNote Yavuz A, Azar İ, Özcan A, Ülkü N (01 Aralık 2021) A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS. Gıda 47 1 25–33.
IEEE A. Yavuz, İ. Azar, A. Özcan, ve N. Ülkü, “A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS”, GIDA, c. 47, sy. 1, ss. 25–33, 2021, doi: 10.15237/gida.GD21124.
ISNAD Yavuz, Arzu vd. “A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS”. Gıda 47/1 (Aralık 2021), 25-33. https://doi.org/10.15237/gida.GD21124.
JAMA Yavuz A, Azar İ, Özcan A, Ülkü N. A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS. GIDA. 2021;47:25–33.
MLA Yavuz, Arzu vd. “A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS”. Gıda, c. 47, sy. 1, 2021, ss. 25-33, doi:10.15237/gida.GD21124.
Vancouver Yavuz A, Azar İ, Özcan A, Ülkü N. A MULTICLASS METHOD FOR THE ANALYSIS OF ANTIBIOTIC RESIDUES IN MILK POWDER AND WHEY POWDER BY UPLC-MS/MS. GIDA. 2021;47(1):25-33.

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