The Investigation of Biofilm Formation by Kluyveromyces lactis and Kluyveromyces lactis drosophilarum on Biopolymer Composite Supports
Öz
The biofilm form of Kluyveromces lactis NRRL Y-8279 and Kluyveromces lactis drosophilarum NRRL Y-8278 were done mature on some biopolymer composite support (BCS) to count colony forming unites (CFU). These supports consisted of (w/w) polypropylene, (w/w) soybean hulls, (w/w) yeast extract, (w/w) soybean flour, and (w/w) bovine albumin. These composites were effectively on the growing of biofilm formation. This form of yeasts was analyzed to use Stripping-Sand method for each one on diverse composites such as BCS1, BCS2, BCS3 and BCS4. Yeasts were showed the visible biofilm formations on supports. Biofilm formation of K. lactis and K. l. drosophilarum were performed 1.2× 109 and 1.6×1010 CFUml-1 on the BCS2. The materials especially polypropylene that were included by BCS2 are critically to produce biofilm formation of yeasts on some applications such as food, biomedical, industries and laboratories. The results of this paper will be usefully on these applications and to notice about the form of polypropylene in the supports how it changes the formation of biofilm by yeasts
Anahtar Kelimeler
Kaynakça
- 75×105 CFU ml-1 Estivill et al. 2011 Estivill et al. 2011 Estivill et al. 2011 Li et al. 2012 Li et al. 2012 Candida albicans Titanium polyethylene Terephthalate Polystyrene
- Staphylococcus aureus 0.405 (OD590) Ciccio et al. 2015 Ciccio et al. 2015 Ciccio et al. 2015
- Staphylococcus epidermdis Stainless steel 0.145 (OD590) Ciccio et al. 2015 Ho et all. 1997 Lactobasillus casei Plastic composite supports Polystyrene Klebsiella 8×108 CFU ml-1 Maldonado et al. 2007 Kubota et al. 2008 Pasvolsky et al. 2014 Demirci et al. 2003 In this study BCS3 Kluyveromces lactis drosiphilarum Kluyveromces lactis 4×107 CFU g-1 BCS2 5×109 CFU ml-1 In this study CONCLUSIONS
- Ph.D. Thesis, Hacettepe University, Ankara, 150s. Almshawit, H., Macreadie, I., Grando, D., 20 A simple and inexpensive device for Microbiol.Methods, 98: 59–63. J. of Bober, J.A., Demirci, A., 2004. Nisin by fermentation subsp.lactis using biopolymer supportss in biofilm reactors. J. of Sci. Res. Dev.
- Vol. VI.Manuscript FP 04 001. Carpentier, B., Cerf, O., 1993. Biofilm and their reference to hygiene in the food industry. J. Appl Bacteriol ., 75: 499-511.
- Chandra, J., Mukherjee, P.K., Ghannoum, M.A., 2008. In vitro growth and analysis of Candida biofilms. Nat. Protoc., 3(12): 1909–1924.
- Costerton, J.W., Stewart, P.S., Greenburg, 19 Bacterial E.P., common cause of persistent infections. Science. 284 (5418):1318-1322.
- Demirci, A., Pometto, A.L., Ho, K.L.G., 1997.
- Ethanol production by Saccharomyces cerevisiae biofilm Ind.Microbiol., 19: 299-304. reactors. J. Demirci, A., Cotton, J.C., Pometto, A.L., Harkins, K.R., Hinz, P.N., 2003.
- Resistance of Lactobacillus casei in plastic- reactors extraction and optimization of the lactic acid Biotechnol.Bioeng. 1002/bit.10722 biofilm liquid membrane extraction system. DOI: 83(7),
- Ciccio, P.D., Vergara, Festino, A.R., Paludi, D., Zanardi, E., Ghidini, S., Ianieri, A., 20 Biofilm formation by Society for Li, J., Hirota, K., Goto, T., Yumoto, H., Miyake, Y., Ichikawa T., 2012. Biofilm formation of Candida albicans on implant overdenture materials and its removal. J. Dent., 40: 686-692.
- Maldonado, N.C., Silva, C.R. Cecilia, M., Macias, M.E.N., 2007. A simple technique to detect Klebsiella biofilm- forming-strains. Inhibitory potential of
- Lactobacillus ermentum CRL 1058 whole Communicating anducational
- Appl.Microbiol., Formatex. products. Research Top.Trends. McDonogh, R., Schaule, G., Flemming, H.C., 19 The permeability of biofouling layers on membranes. J.Membr. Sci., 87: 199-2
- Mortensen, K.P., Conley, S.N., 1994. Film fill fouling in counterflow cooling towers: mechanisms and design. CTI J., 15:10
- Meyer, A., Wallis, F.M., 1997. Development of microbial biofilms on various surfaces for the treatment of heavy metal containing Tech., 11(12): 859-863. Biotechnol
- Nasib, Q., Bassam, A.A., Thaddeus, C.E., Patrick, K., Lan, S.M., 2005. Biofilm reactors for industrial bioconversion processes: enhanced reaction rates. Microb. Cell Factor., 4: 24 doi:10.1186/1475-2859-4- potential of
- Nett, J.E., Andes, D., 2006.Candida albicans biofilm development, modeling a host– pathogen
- Curr.OpinMicrobiol., 9: 340–344. Pasvolsky, R., Zakin, V., Ostrova, I., Shemesh, M., 2014. Butyric acid released during milk lipolysis triggers biofilm formation of Bacillus species,
- Int. J. Food Microbiol., 181: 19–27 Saçak, M., 2004. Polimer kimyası. Gazi Kitabevi, Ankara.
- Taras, M., Hakansson, K., Guieysse, B., 200 Continuous degradation in packed-bed bioreactor. Appl. Microbiol. Biotechno., 66: 567-574
- Uppuluri, P., Lopez-Ribot, J.L., 2010. An easy and economical in vitro method for the formation of Candida albicans biofilms under continuous conditions of flow. Virulence, 1: 483–487.
- Yönten, V., 2010. The investigation of species of yeasts formed biofilms. Ph.D. Thesis,
- Yüzüncü Yıl University, 2010, Van, 134s.
Kluyveromces Lactis ve Kluyveromces Lactis Drosophilarum’un Biyofilm Yapılarının Biyopolimer Kompozit Destekler Üzerindeki Araştırılması
Öz
Kluyveromces lactis NRRL Y-8279 ve Kluyveromces lactis drosophilarum
NRRL Y-8278 mayalarının biyofilm yapısı bazı biyopolimer material destekler
üzerinde geliştirilip, bu kültürlerin biyofilm ölçümleri birim alandaki koloni sayıları
analiz edilerek hesaplanmıştır. Bu destekler ağırlık olarak (w/w) polipropilen, soya
tanesi, maya özütü, soya tozu, sığır albumin ve mineral tuzlardan oluşmuşlardır. İlgili
materyaller biyofilm gelişiminde önemli bir etkiye sahiptirler. Mayaların bu özelliği
(biyofilm yapısı) Stripping-Sand metodu kullanılarak her bir destek için (BCS1, BCS2,
BCS3 ve BCS4) ayrı ayrı hesaplanmıştır. Araştırmalar mayaların ilgili destekler
üzerinde gözle görünebilir biyofilm oluşturduklarını gösterdi. K. lactis ve K. l.
drosophilarum kültürleri üzerinde biyofilm ölçümü sırası ile BCS2 desteği üzerinde
1.2× 109 ve 1.6×1010 CFUml-1 olarak hesap edildi. Özellikle bu desteğin içerdiği
polipropilenin kullanıldığı gıda, biyomedikal, endüstri ve laboratuar gibi bazı alanlarda
oluşacak biyofilm yapısında önemli bir yere sahip olduğu görülmüştür. Bu çalışmanın
sonucu bazı uygulamalarda çok yararlı olacak ve günümüzde mayaların biyofilm
yapıları oluşturmaları için polipropilen gibi destekler üzerine dikkatleri çekecektir.
Anahtar Kelimeler
Kaynakça
- 75×105 CFU ml-1 Estivill et al. 2011 Estivill et al. 2011 Estivill et al. 2011 Li et al. 2012 Li et al. 2012 Candida albicans Titanium polyethylene Terephthalate Polystyrene
- Staphylococcus aureus 0.405 (OD590) Ciccio et al. 2015 Ciccio et al. 2015 Ciccio et al. 2015
- Staphylococcus epidermdis Stainless steel 0.145 (OD590) Ciccio et al. 2015 Ho et all. 1997 Lactobasillus casei Plastic composite supports Polystyrene Klebsiella 8×108 CFU ml-1 Maldonado et al. 2007 Kubota et al. 2008 Pasvolsky et al. 2014 Demirci et al. 2003 In this study BCS3 Kluyveromces lactis drosiphilarum Kluyveromces lactis 4×107 CFU g-1 BCS2 5×109 CFU ml-1 In this study CONCLUSIONS
- Ph.D. Thesis, Hacettepe University, Ankara, 150s. Almshawit, H., Macreadie, I., Grando, D., 20 A simple and inexpensive device for Microbiol.Methods, 98: 59–63. J. of Bober, J.A., Demirci, A., 2004. Nisin by fermentation subsp.lactis using biopolymer supportss in biofilm reactors. J. of Sci. Res. Dev.
- Vol. VI.Manuscript FP 04 001. Carpentier, B., Cerf, O., 1993. Biofilm and their reference to hygiene in the food industry. J. Appl Bacteriol ., 75: 499-511.
- Chandra, J., Mukherjee, P.K., Ghannoum, M.A., 2008. In vitro growth and analysis of Candida biofilms. Nat. Protoc., 3(12): 1909–1924.
- Costerton, J.W., Stewart, P.S., Greenburg, 19 Bacterial E.P., common cause of persistent infections. Science. 284 (5418):1318-1322.
- Demirci, A., Pometto, A.L., Ho, K.L.G., 1997.
- Ethanol production by Saccharomyces cerevisiae biofilm Ind.Microbiol., 19: 299-304. reactors. J. Demirci, A., Cotton, J.C., Pometto, A.L., Harkins, K.R., Hinz, P.N., 2003.
- Resistance of Lactobacillus casei in plastic- reactors extraction and optimization of the lactic acid Biotechnol.Bioeng. 1002/bit.10722 biofilm liquid membrane extraction system. DOI: 83(7),
- Ciccio, P.D., Vergara, Festino, A.R., Paludi, D., Zanardi, E., Ghidini, S., Ianieri, A., 20 Biofilm formation by Society for Li, J., Hirota, K., Goto, T., Yumoto, H., Miyake, Y., Ichikawa T., 2012. Biofilm formation of Candida albicans on implant overdenture materials and its removal. J. Dent., 40: 686-692.
- Maldonado, N.C., Silva, C.R. Cecilia, M., Macias, M.E.N., 2007. A simple technique to detect Klebsiella biofilm- forming-strains. Inhibitory potential of
- Lactobacillus ermentum CRL 1058 whole Communicating anducational
- Appl.Microbiol., Formatex. products. Research Top.Trends. McDonogh, R., Schaule, G., Flemming, H.C., 19 The permeability of biofouling layers on membranes. J.Membr. Sci., 87: 199-2
- Mortensen, K.P., Conley, S.N., 1994. Film fill fouling in counterflow cooling towers: mechanisms and design. CTI J., 15:10
- Meyer, A., Wallis, F.M., 1997. Development of microbial biofilms on various surfaces for the treatment of heavy metal containing Tech., 11(12): 859-863. Biotechnol
- Nasib, Q., Bassam, A.A., Thaddeus, C.E., Patrick, K., Lan, S.M., 2005. Biofilm reactors for industrial bioconversion processes: enhanced reaction rates. Microb. Cell Factor., 4: 24 doi:10.1186/1475-2859-4- potential of
- Nett, J.E., Andes, D., 2006.Candida albicans biofilm development, modeling a host– pathogen
- Curr.OpinMicrobiol., 9: 340–344. Pasvolsky, R., Zakin, V., Ostrova, I., Shemesh, M., 2014. Butyric acid released during milk lipolysis triggers biofilm formation of Bacillus species,
- Int. J. Food Microbiol., 181: 19–27 Saçak, M., 2004. Polimer kimyası. Gazi Kitabevi, Ankara.
- Taras, M., Hakansson, K., Guieysse, B., 200 Continuous degradation in packed-bed bioreactor. Appl. Microbiol. Biotechno., 66: 567-574
- Uppuluri, P., Lopez-Ribot, J.L., 2010. An easy and economical in vitro method for the formation of Candida albicans biofilms under continuous conditions of flow. Virulence, 1: 483–487.
- Yönten, V., 2010. The investigation of species of yeasts formed biofilms. Ph.D. Thesis,
- Yüzüncü Yıl University, 2010, Van, 134s.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Mühendislik |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 7 Eylül 2015 |
| Gönderilme Tarihi | 7 Eylül 2015 |
| Yayımlandığı Sayı | Yıl 2015 Cilt: 1 Sayı: 1 |
