Fe2O3, TiO2 VE ZnO NANOPARÇACIKLARININ CHLAMYDOMONAS REINHARDTII ÜZERİNE ETKİSİNİN İNCELENMESİ

Yıl 2024, Cilt: 12 Sayı: 1, 289 - 304, 19.02.2024

Nesrin Özmen

https://doi.org/10.33715/inonusaglik.1401595

Öz

Bu çalışmada tek hücreli bir alg olan Chlamydomonas reinhardtii’nin Fe2O3, TiO2, ve ZnO NPların farklı konsantrasyonlarına (Fe2O3ve TiO2 için 1.8-61.22 mg/L, ZnO için 0.39-10.48 mg/L aralığında) 24, 72 ve 120 saat süre ile maruz bırakması sonucu ortaya çıkan toksik etkilerin değerlendirilmesi amaçlandı. Bu NP konsantrasyonları ön testlere göre belirlendi. Toksisitenin ölçütü olarak, NP’lerin kullanılan her konsantrasyonu için belirtilen süre sonunda kültür ortamlarında toplam hücre sayıları, ortamdaki canlı hücre sayıları, toplam hücre kütlesi ve hücre boyutlarındaki değişim kullanıldı. Sonuçlar istatistiksel olarak değerlendirilmiş ve kullanılan her üç nanoparçacığın da kültürdeki toplam hücre sayısını azalttığı (Ti< Fe

Anahtar Kelimeler

Metal Nanoparçacıklar, Chlamydomonas reinhardtii, Toksisite, Titanyum Dioksit, Demir (III) Oksit, Çinko Oksit

Investigation of the Effect of Fe2O3, TiO2 and ZnO Nanoparticles on Microalgea Chlamydomonas reinhardtii

Öz

The aim of this study was to evaluate the toxic effects of Chlamydomonas reinhardtii, a single-celled alga, exposed to different concentrations of Fe2O3, TiO2, and ZnO NPs (1.8-61.22 mg/L for Fe2O3 and TiO2, 0.39-10.48 mg/L for ZnO) for 24, 72 and 120 hours. These NP concentrations were determined according to preliminary tests. As a measure of toxicity, the total number of cells in the culture media, the number of viable cells in the media, the total cell mass and the change in cell size at the end of the specified time for each concentration of NPs used were used. The results were statistically evaluated and it was observed that all three nanoparticles used decreased the total number of cells in culture (Ti< Fe

Anahtar Kelimeler

Metal Nanoparticles, Chlamydomonas reinhardtii, Toxicity, Titanium Dioxide, Iron (III) Oxide, Zinc Oxide

Kaynakça

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