Year 2017, Volume 38, Issue 3, Pages 516 - 524 2017-09-30

Evernia Prunastri’nin Antiproliferatif Aktivitesi ve Hücresel Apoptotik Yolak Arasindaki İlişki
Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway

Önder YUMRUTAŞ [1] , Celal GÜVEN [2] , Yusuf ÖZAY [3] , Işıl ALBENİZ [4] , Müfide AYDOĞAN AHBAB [5] , İbrahim BOZGEYİK [6] , Atilla YILDIZ [7] , Haydar BAĞIŞ [8] , Leyla TÜRKER ŞENER [9]

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Parmeliaceae’ye ait olan Evernia prunastri (L.) Ach, Türkiye yetişen önemli bir liken türüdür. önceki çalışmalarda E. Prunastri’nin yapısında bulanan kimyasallardan dolayı antioksidan, antimikrobiyal ve antikanser aktivitelere sahip oldukları belirtilmiştir. E. Prunastri’nin bazı kanser hücre tiplerinde antiproliferatif aktiviteleri bilinmekle birlikte bu aktivitenin yolağı hakkında bilgilerimiz oldukça sınırlıdır. Bundan dolayı, çalışmamızda E. Prunastri ekstraktının antiproliferatif etkileri ve hücresel apoptotik yolak arasında ilişkinin belirlenmesi amaçlanmıştır. Bu amaç için, meme kanseri hücrelerini E. Prunastri ekstraktlarına maruz bırakılmış ve hücre canlılığı MTT boyama ve xCELLigence system ile belirlenmiştir. Annexine V/PI boyama ile Apoptozun indüksiyonu, westernblot ile apoptotik proteinlerin ekspresyonu ve floresan boyama ile aktin flamentlerinin hücresel yoğunluğu belirlenmiştir. E. Prunastri MeOH ekstraktının yüksek dozlarda antiproliferatif etkiler göstermiştir (1, 5 ve 25 µg ml-1 için p<0.05 ; 125 µg ml-1 için p<0.01). Ancak ekstrakt uygulaması sonrasında apoptozun indüklenmediği ve apoptotik protein seviyelerinin değişmediği belirlenmiştir. Ayrıca F-aktin seviyesininde değişmediği gözlemlenmiştir. Sonuç olarak, E. Prunastri MeOH ekstraktının meme kanseri hücreleri üzerine antiproliferatif bir etkiye sahip olduğu ancak bu etkinin apoptotik yolakla ilişkili olmadığı düşünülmektedir.

Evernia prunastri (L.) Ach, belonging to Parmeliaceae, is an important lichen species in Turkey. Previous studies was reported that E. prunastri have significant antioxidant, antimicrobial and anticancer compounds in its structure. Although antiproliferative effects of E. prunastri are determined in some types of cancer, there is little information about the pathway of this activity. Accordingly, we aimed to determine the association between anti-proliferative activities of E. prunastri extracts with the cellular apoptotic pathway. Determination of cell viability was assessed by MTT assay. The xCELLigence system was used to monitor cell proliferation. To reveal induction apoptosis, expression of apoptotic pathway proteins, F-actin staining, ANNEXIN V/propodium iodide (PI) staining was used. As a result, E. prunastri MeOH extracts were found to have anti-proliferative activities especially in high doses (p<0.05 for 1, 5 and 25 µg ml-1; p<0.01 for 125 µg ml-1). Also, the extracts were found to be not inducing apoptosis in flow cytometric measurements. In addition, there was no significant decrease or increase in protein expressions of apoptotic pathway proteins. Lastly, density of actin filaments was not affected. MeOH extract of E. prunastri was found to have anti-proliferative activities, yet these anti-proliferative activities were not related to cellular apoptotic pathways.

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Subjects Basic Sciences
Journal Section Articles
Authors

Author: Önder YUMRUTAŞ

Author: Celal GÜVEN

Author: Yusuf ÖZAY

Author: Işıl ALBENİZ

Author: Müfide AYDOĞAN AHBAB

Author: İbrahim BOZGEYİK

Author: Atilla YILDIZ

Author: Haydar BAĞIŞ

Author: Leyla TÜRKER ŞENER

Bibtex @research article { csj340502, journal = {Cumhuriyet Science Journal}, issn = {2587-2680}, eissn = {2587-246X}, address = {Cumhuriyet University}, year = {2017}, volume = {38}, pages = {516 - 524}, doi = {10.17776/csj.340502}, title = {Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway}, key = {cite}, author = {YUMRUTAŞ, Önder and GÜVEN, Celal and ÖZAY, Yusuf and ALBENİZ, Işıl and AYDOĞAN AHBAB, Müfide and BOZGEYİK, İbrahim and YILDIZ, Atilla and BAĞIŞ, Haydar and TÜRKER ŞENER, Leyla} }
APA YUMRUTAŞ, Ö , GÜVEN, C , ÖZAY, Y , ALBENİZ, I , AYDOĞAN AHBAB, M , BOZGEYİK, İ , YILDIZ, A , BAĞIŞ, H , TÜRKER ŞENER, L . (2017). Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway. Cumhuriyet Science Journal, 38 (3), 516-524. DOI: 10.17776/csj.340502
MLA YUMRUTAŞ, Ö , GÜVEN, C , ÖZAY, Y , ALBENİZ, I , AYDOĞAN AHBAB, M , BOZGEYİK, İ , YILDIZ, A , BAĞIŞ, H , TÜRKER ŞENER, L . "Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway". Cumhuriyet Science Journal 38 (2017): 516-524 <http://dergipark.org.tr/csj/issue/31252/340502>
Chicago YUMRUTAŞ, Ö , GÜVEN, C , ÖZAY, Y , ALBENİZ, I , AYDOĞAN AHBAB, M , BOZGEYİK, İ , YILDIZ, A , BAĞIŞ, H , TÜRKER ŞENER, L . "Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway". Cumhuriyet Science Journal 38 (2017): 516-524
RIS TY - JOUR T1 - Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway AU - Önder YUMRUTAŞ , Celal GÜVEN , Yusuf ÖZAY , Işıl ALBENİZ , Müfide AYDOĞAN AHBAB , İbrahim BOZGEYİK , Atilla YILDIZ , Haydar BAĞIŞ , Leyla TÜRKER ŞENER Y1 - 2017 PY - 2017 N1 - doi: 10.17776/csj.340502 DO - 10.17776/csj.340502 T2 - Cumhuriyet Science Journal JF - Journal JO - JOR SP - 516 EP - 524 VL - 38 IS - 3 SN - 2587-2680-2587-246X M3 - doi: 10.17776/csj.340502 UR - https://doi.org/10.17776/csj.340502 Y2 - 2017 ER -
EndNote %0 Cumhuriyet Science Journal Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway %A Önder YUMRUTAŞ , Celal GÜVEN , Yusuf ÖZAY , Işıl ALBENİZ , Müfide AYDOĞAN AHBAB , İbrahim BOZGEYİK , Atilla YILDIZ , Haydar BAĞIŞ , Leyla TÜRKER ŞENER %T Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway %D 2017 %J Cumhuriyet Science Journal %P 2587-2680-2587-246X %V 38 %N 3 %R doi: 10.17776/csj.340502 %U 10.17776/csj.340502
ISNAD YUMRUTAŞ, Önder , GÜVEN, Celal , ÖZAY, Yusuf , ALBENİZ, Işıl , AYDOĞAN AHBAB, Müfide , BOZGEYİK, İbrahim , YILDIZ, Atilla , BAĞIŞ, Haydar , TÜRKER ŞENER, Leyla . "Association Between Anti-Proliferative Activity of Evernia Prunastri with the Cellular Apoptotic Pathway". Cumhuriyet Science Journal 38 / 3 (September 2017): 516-524. https://doi.org/10.17776/csj.340502