Year 2019, Volume 7, Issue 3, Pages 1279 - 1289 2019-07-31

Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü
In Vivo Protective Role of Nigella sativa L. Against Pb(NO3)2 Induced Toxicity

Kürşad YAPAR [1] , Kültiğin ÇAVUŞOĞLU [2] , Emine YALÇIN [3] , Ali ACAR [4] , Güray DEMİRTAŞ [5]

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In this study, the protective role of Nigella sativa L. seed extract (NSSE) against Pb(NO3)2 toxicity in albino mice was investigated. For this purpose, the mice were randomly divided to six groups. In control group, mice were treated with tap water and in the treatment groups 500 mg kg-1 bw Pb(NO3)2, 300 and 600 mg kg-1 bw doses of NSSE were exposed to mice. The effects of all treatments on organism development were investigated by determining the changes in body, liver and kidney weights of each group. Genotoxic effects were determined by investigating the chromosomal abnormalities (CAs) in bone marrow cells, micronucleus (MN) frequency in erythrocyte and buccal mucosa cells. As a result, it was observed that Pb(NO3)2  treatment resulted in a 6.33-fold decrease in body weight gain and 2.08 and 1.65-fold increase in liver and kidney weights compared to control group, respectively. In addition, it was determined that these alterations in weights were positively changed after NSSE treatment. From MN assays it was observed that MN frequencies of erythrocyte and buccal mucosa cells in 500 mg kg-1 bw Pb(NO3)2 treated group were found as 58.17±5.46 and 19.00±2.76 while in 600 mg kg-1 bw NSSE+Pb(NO3)2 treated group, the frequencies were determined as 32.67±3.78 and 6.50±1.87, respectively. A similar protective role was also observed against CAs formation, and 600 mg kg-1 NSSE treatment was found to reduce the chromatid breaks by 44%. As a result, it has been determined that NSSE can be used as a protective nutrient against the harmful effects of chemicals such as heavy metals especially Pb.

Albino mice, Body weight, Cytotoxicity, Nigella sativa, Organ weight
  • [1] N. Saglam and N. Cihangir, “The Studies on Biosorption of Heavy Metals by Biological Processes,” Hacettepe University Journal of Education, vol. 11, pp. 157-161, 1995.
  • [2] P. B. Tchounwou, C. G. Yedjou, A. K. Patlolla and D. J. Sutton, “Heavy Metal Toxicity and the Environment,” Molecular, Clinical and Environmental Toxicology, vol. 101, pp. 133-164, 2012.
  • [3] J. A. Centeno, R. B. Finkelman and O. Selinus, “Medical Geology: Impacts of the Natural Environment on Public Health,” Geosciences, vol. 4, pp. 114-127, 2014.
  • [4] H. Gurer and N. Ercal, “Can Antioxidants be Beneficial in the Treatment of Lead Poisoning?,” Free Radical Biology and Medicine, vol. 29, no. 10, pp. 927-945, 2000.
  • [5] B. Seven, E. Yalcin, A. Acar, K. Yapar and K. Cavusoglu, “Investigation of Genotoxicity Caused by Paraben in Albino Mice: Protective Role of Nettle Extract,” Cumhuriyet Science Journal, vol. 38, no. 3, pp. 572-580, 2017.
  • [6] E. Yalcin, A. Acar and K. Cavusoglu, “Investigation of the Protective Role of Carotene Against Toxicity Encouraged by Ammonium Sulfate in Albino Mice,” Düzce University Journal of Science & Technology, vol. 5, no. 1, pp. 273-280, 2017.
  • [7] M. Burits and F. Bucar, “Antioxidant Activity of Nigella sativa Essential Oil,” Phytotherapy Research, vol. 14, no. 5, pp. 323-328, 2000.
  • [8] C. Nergiz and S. Otles, “Chemical Composition of Nigella sativa L. Seeds,” Food Chemistry, vol. 48, no. 3, pp. 259-261, 1993.
  • [9] M. Mahfouz and M. El-Dakhakhny, “The Isolation of a Crystalline Active Principle from Nigella sativa L. Seeds,” Journal of Pharmaceutical Sciences, vol. 1, pp. 1-19, 1960.
  • [10] International Ethical Guidelines for Epidemiological Studies” repared by the Council for International Organizations of Medical Sciences (CIOMS) in collaboration with the World Health Organization (WHO). February 2008. Geneva.
  • [11] T. H. Ma, X. Zhou, G. F. Loarco, G. G. Arreola and S. U. Lecona, “Mouse–Erythrocyte Micronucleus (Mus-EMN) Assay on the Clastogenicity of Industrial Wastewater,” Rev. Int. Contam. Ambient, vol. 11, no. 2, pp. 95-98, 1995.
  • [12] M. Fenech, W. P. Chang, M. Kirsch-Volders, N. Holland, S. Bonassi and E. Zeiger, “HUMN project: Detailed Description of the Scoring Criteria for the Cytokinesis-block Micronucleus Assay Using Isolated Human Lymphocyte Cultures,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 534, no. 1-2, pp. 65-75, 2003.
  • [13] J. R. Savage, “Classification and Relationships of Induced Chromosomal Structual Changes,” Journal of Medical Genetics, vol. 13, no. 2, pp. 103-122, 1976.
  • [14] S. J. S. Flora, M. Mittal and A. Mehta, “Heavy Metal Induced Oxidative Stress & Its Possible Reversal by Chelation Therapy,” Indian Journal of Medical Research, vol. 128, no. 4, pp. 501-523, 2008.
  • [15] K. P. Woodward. “Growth, Cellular Development of Skeletal Muscle and Body Composition of Rats Selected for Post Weaning gain,” Retrospective Theses and Dissertations, vol. 6254, 1976.
  • [16] T. C. King. “Cell Injury, Cellular Responses to Injury and Cell Death,” in Elsevier's Integrated Pathology, 2007.
  • [17] A. P. Yagminas, C. A. Franklin, D. C. Villeneuve, A. P. Gilman, P. B. Little and V. E. O. Valli, “Subchronic Oral Toxicity of Triethyl Lead in the Male Weanling Rat. Clinical, Biochemical, Hematological, and Histopathological Effects,” Toxicological Sciences, vol. 15, no. 3, pp. 580-596, 1990.
  • [18] A. Ahmad, A. Husain, M. Mujeeb, S. A. Khan, A. K. Najmi, N. A. Siddique, Z. A. Damanhouri and F. Anwar, “A Review on Therapeutic Potential of Nigella sativa: A Miracle Herb,” Asian Pacific Journal of Tropical Biomedicine, vol. 3, no. 5, pp. 337-352, 2013.
  • [19] J. T. Tapisso, C. C. Marques, M. da Luz Mathias and M. da Graca Ramalhinho, “Induction of Micronuclei and Sister Chromatid Exchange in Bone-Marrow Cells and Abnormalities in Sperm of Algerian Mice (Mus spretus) Exposed to Cadmium, Lead and Zinc,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 678, no. 1, pp. 59-64, 2009.
  • [20] G. C. Jagetia and R. Aruna, “Effect of Various Concentrations of Lead Nitrate on the Induction of Micronuclei in Mouse Bone Marrow,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 415, no. 1, 131-137, 1998.
  • [21] M. Von Ledebur and W. Schmid, “The Micronucleus Test Methodological Aspects,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 19, no. 1, pp. 109-117, 1973.
  • [22] K. Mortelmans, and D. S. Rupa, “Current Issues in Genetic Toxicology Testing for Microbiologists,” Advances in Applied Microbiology, vol. 56, no. 3, pp. 379-397, 2004.
  • [23] E. I. Aboul-Ela, “Cytogenetic Studies on Nigella sativa Seeds Extract and Thymoquinone on Mouse Cells Infected with Schistosomiasis Using Karyotyping,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 516, no. 1, pp. 11-17, 2002.
  • [24] A. Kasperczyk, L. Słowińska-Łożyńska, M. Dobrakowski, J. Zalejska-Fiolka and S. Kasperczyk, “The Effect of Lead-Induced Oxidative Stress on Blood Viscosity and Rheological Properties of Erythrocytes in Lead Exposed Humans,” Clinical Hemorheology and Microcirculation, vol. 56, no. 3, pp. 187-195, 2014.
  • [25] S. Nehar and M. Kumari. “Ameliorating Effect of Nigella sativa oil on Thioacetamide-induced Liver Cirrohsis in Albino Rats,” Indian Journal of Pharmaceutical Education and Research, vol. 47, pp. 135-139, 2013.
  • [26] D. Canayakin, Y. Bayir, N. Kilic Baygutalp, K. Sezen, E. Karaoglan, H. Atmaca, F.B. Kocak Ozgeris, M.S. Keles and Z. Halici. “Paracetamol-induced nephrotoxicity and oxidative stress in rats: the protective role of Nigella sativa,” Pharmaceutical Biology, vol. 54, no. 10, pp. 2082-2091, 2016.
  • [27] A. Mabrouk and H. Cheikh. “Thymoquinone supplementation reverses lead-induced oxidative stress in adult rat testes,” General Physiology and Biophysics, vol. 34, pp.65–72, 2015.
  • [28] M. J. Salomi, S. C. Nair and K. R. Panikkar, “Inhibitory Effects of Nigella sativa and Saffron (Crocus sativus) on Chemical Carcinogenesis in Mice,” Nutrition and Cancer, vol. 16, no. 1, pp. 67-72, 1991.
  • [29] M. Tariq, “Nigella sativa Seeds: Folklore Treatment in Modern Day Medicine,” Saudi Journal of Gastroenterology, vol. 14, no. 3, pp. 105-106, 2008.
  • [30] E. Lev and Z. Amar, “Ethnopharmacological Survey of Traditional Drugs Sold in Israel at the end of the 20th Century,” Journal of Ethnopharmacology, vol. 72, no. 1-2, pp. 191-205, 2000.
  • [31] O. A. Badary, R. A. Taha, A. M. Gamal El-Din and M. H. Abdel-Wahab, “Thymoquinone is a Potent Superoxide Anion Scavenger,” Drug and Chemical Toxicology, vol. 26, no. 2, pp. 87-98, 2003.
Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0003-0287-4521
Author: Kürşad YAPAR
Institution: Giresun University
Country: Turkey


Orcid: 0000-0002-4767-9132
Author: Kültiğin ÇAVUŞOĞLU (Primary Author)
Institution: GİRESUN RESEARCH CENTER
Country: Turkey


Orcid: 0000-0002-5280-5375
Author: Emine YALÇIN
Institution: GİRESUN RESEARCH CENTER

Orcid: 0000-0001-8617-2206
Author: Ali ACAR
Institution: GİRESUN RESEARCH CENTER
Country: Turkey


Orcid: 0000-0002-3853-5524
Author: Güray DEMİRTAŞ
Institution: Giresun University
Country: Turkey


Dates

Publication Date: July 31, 2019

Bibtex @research article { dubited546146, journal = {Düzce Üniversitesi Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2446}, address = {Duzce University}, year = {2019}, volume = {7}, pages = {1279 - 1289}, doi = {10.29130/dubited.546146}, title = {Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü}, key = {cite}, author = {YAPAR, Kürşad and ÇAVUŞOĞLU, Kültiğin and YALÇIN, Emine and ACAR, Ali and DEMİRTAŞ, Güray} }
APA YAPAR, K , ÇAVUŞOĞLU, K , YALÇIN, E , ACAR, A , DEMİRTAŞ, G . (2019). Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 7 (3), 1279-1289. DOI: 10.29130/dubited.546146
MLA YAPAR, K , ÇAVUŞOĞLU, K , YALÇIN, E , ACAR, A , DEMİRTAŞ, G . "Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 (2019): 1279-1289 <http://dergipark.org.tr/dubited/issue/46290/546146>
Chicago YAPAR, K , ÇAVUŞOĞLU, K , YALÇIN, E , ACAR, A , DEMİRTAŞ, G . "Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 (2019): 1279-1289
RIS TY - JOUR T1 - Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü AU - Kürşad YAPAR , Kültiğin ÇAVUŞOĞLU , Emine YALÇIN , Ali ACAR , Güray DEMİRTAŞ Y1 - 2019 PY - 2019 N1 - doi: 10.29130/dubited.546146 DO - 10.29130/dubited.546146 T2 - Düzce Üniversitesi Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 1279 EP - 1289 VL - 7 IS - 3 SN - -2148-2446 M3 - doi: 10.29130/dubited.546146 UR - https://doi.org/10.29130/dubited.546146 Y2 - 2019 ER -
EndNote %0 Duzce University Journal of Science and Technology Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü %A Kürşad YAPAR , Kültiğin ÇAVUŞOĞLU , Emine YALÇIN , Ali ACAR , Güray DEMİRTAŞ %T Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü %D 2019 %J Düzce Üniversitesi Bilim ve Teknoloji Dergisi %P -2148-2446 %V 7 %N 3 %R doi: 10.29130/dubited.546146 %U 10.29130/dubited.546146
ISNAD YAPAR, Kürşad , ÇAVUŞOĞLU, Kültiğin , YALÇIN, Emine , ACAR, Ali , DEMİRTAŞ, Güray . "Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 / 3 (July 2019): 1279-1289. https://doi.org/10.29130/dubited.546146
AMA YAPAR K , ÇAVUŞOĞLU K , YALÇIN E , ACAR A , DEMİRTAŞ G . Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü. DUBİTED. 2019; 7(3): 1279-1289.
Vancouver YAPAR K , ÇAVUŞOĞLU K , YALÇIN E , ACAR A , DEMİRTAŞ G . Pb(NO3)2 Toksisitesine Karşı Nigella sativa L.’nin In vivo Koruyucu Rolü. Düzce Üniversitesi Bilim ve Teknoloji Dergisi. 2019; 7(3): 1289-1279.