Year 2018, Volume , Issue 14, Pages 272 - 279 2018-12-31

Astragalus trojanus Kesikli Kültürlerinde Prekürsör ve Elisitör Kullanımının Optimizasyonu
Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev.

Pınar Nartop [1] , Aynur Gürel [2] , İsmail Hakkı Akgün [3] , Erdal Bedir [4]

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Elisitör ve prekürsör uygulamaları bitki hücre kültürlerinde sekonder metabolizmayı hızlandırmak için sıklıkla kullanılan uygulamalardır. Bu çalışmada Astragalus trojanus kesikli kültürlerinde astragalozit IV ve sikloastragenol üretimlerinin arttırılması için metil jasmonat, jasmonik asit, salisilik asit ve pektin elisitör olarak, β-sitosterol ise prekürsör olarak kullanılmıştır. Kesikli kültürlerin büyüme parametreleri (yaş ve kuru ağırlıklar ve kuru ağırlık yüzdeleri) elisitörler ve prekürsörün primer metabolizma üzerine etkilerinin belirlenmesi için değerlendirilmiştir. Elisitörler ve prekürsörün kültür periyodunun farklı basamaklarında metabolit üretimini tetiklediği belirlenmiştir. En yüksek astragalozit IV birikimi (0.9435 µg/mg) 50 µM metil jasmonat içeren besin ortamında kültürün 14. gününde görülürken, en yüksek sikloastragalozit konsantrasyonu 50 µM jasmonik asit içeren besin ortamnda kültürün 28. gününde tespit edilmiştir. Çalışmada büyük ölçekli kültivasyon da gerçekleştirilmiştir ve 50 µM metil jasmonat içeren besin ortamında 14. günde 0.3759 µg/mg astragalozit IV birikimi tespit edilmiştir.

Elicitor and precursor applications are commonly used to induce secondary metabolism in plant cell cultures. In this study, methyl jasmonate, jasmonic acid, salicylic acid and pectin were used as elicitors and β-sitosterol was used as a precursor in batch cultures of Astragalus trojanus in order to trigger astragaloside IV and cycloastragenol productions. Growth parameters (fresh and dry weights and dry weight percentages) of batch cultures were also evaluated in order to understand the effects of elicitors and precursor on primary metabolism. All elicitors and precursor used in this study triggered metabolite production at different stages of culture period. The highest astragaloside IV accumulation (0.9435 µg/mg) was detected in medium supplemented with 50 µM methyl jasmonate at the 14th day of culture period, whereas the highest cycloastragenol concentration (0.3626 µg/mg) was found in medium supplemented with 50 µM jasmonic acid at the 28th day of culture period. Large scale cultivation was also performed and 0.3759 µg/mg astragaloside IV was detected in medium supplemented with 50 µM methyl jasmonate at the 14th day.

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Primary Language en
Subjects Engineering
Journal Section Makaleler
Authors

Orcid: 0000-0003-2765-6133
Author: Pınar Nartop (Primary Author)
Institution: ÇORLU FACULTY OF ENGINEERING
Country: Turkey


Author: Aynur Gürel

Author: İsmail Hakkı Akgün

Author: Erdal Bedir

Dates

Publication Date: December 31, 2018

Bibtex @research article { ejosat461308, journal = {Avrupa Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2683}, address = {Osman SAĞDIÇ}, year = {2018}, volume = {}, pages = {272 - 279}, doi = {10.31590/ejosat.461308}, title = {Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev.}, key = {cite}, author = {Nartop, Pınar and Gürel, Aynur and Akgün, İsmail Hakkı and Bedir, Erdal} }
APA Nartop, P , Gürel, A , Akgün, İ , Bedir, E . (2018). Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev.. Avrupa Bilim ve Teknoloji Dergisi, (14), 272-279. DOI: 10.31590/ejosat.461308
MLA Nartop, P , Gürel, A , Akgün, İ , Bedir, E . "Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev.". Avrupa Bilim ve Teknoloji Dergisi (2018): 272-279 <http://dergipark.org.tr/ejosat/issue/40225/461308>
Chicago Nartop, P , Gürel, A , Akgün, İ , Bedir, E . "Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev.". Avrupa Bilim ve Teknoloji Dergisi (2018): 272-279
RIS TY - JOUR T1 - Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev. AU - Pınar Nartop , Aynur Gürel , İsmail Hakkı Akgün , Erdal Bedir Y1 - 2018 PY - 2018 N1 - doi: 10.31590/ejosat.461308 DO - 10.31590/ejosat.461308 T2 - Avrupa Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 272 EP - 279 VL - IS - 14 SN - -2148-2683 M3 - doi: 10.31590/ejosat.461308 UR - https://doi.org/10.31590/ejosat.461308 Y2 - 2018 ER -
EndNote %0 European Journal of Science and Technology Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev. %A Pınar Nartop , Aynur Gürel , İsmail Hakkı Akgün , Erdal Bedir %T Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev. %D 2018 %J Avrupa Bilim ve Teknoloji Dergisi %P -2148-2683 %V %N 14 %R doi: 10.31590/ejosat.461308 %U 10.31590/ejosat.461308
ISNAD Nartop, Pınar , Gürel, Aynur , Akgün, İsmail Hakkı , Bedir, Erdal . "Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev.". Avrupa Bilim ve Teknoloji Dergisi / 14 (December 2019): 272-279. https://doi.org/10.31590/ejosat.461308
AMA Nartop P , Gürel A , Akgün İ , Bedir E . Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev.. Avrupa Bilim ve Teknoloji Dergisi. 2018; (14): 272-279.
Vancouver Nartop P , Gürel A , Akgün İ , Bedir E . Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev.. Avrupa Bilim ve Teknoloji Dergisi. 2018; (14): 279-272.