Exploring Apis mellifera L. Venom's Antioxidant Power in Various Solvents: Unveiling its In Vitro Potential

Barış Denk

doi:10.30607/kvj.1343130

TR EN

Apis mellifera L. Zehirinin Çeşitli Solventlerdeki Antioksidan Gücü: In Vitro Potansiyelin Ortaya Çıkarılması

Abstract

Apis mellifera L. zehiri, antioksidan özelliklere sahip biyoaktif bileşenler içermektedir. Çeşitli polar çözücülerde seyreltilen zehir, terapötik amaçlar için kullanılmaktadır. Bu çalışma, standart ham zehir (SV) ve üreticiden temin edilen zehir (BV) örneklerinin in vitro antioksidan aktivitelerini (AOA) belirlemeyi amaçlamaktadır. Bu amaçla örnekler, 1.95-500 µg.ml-1 konsantrasyon aralığında distile su, fizyolojik tuzlu su ve PBS içinde çözülmüştür. Antioksidan aktivitelerin değerlendirilmesi için serbest radikal giderme aktivitesi (DPPH) ve metal şelasyon aktivitesi (MCA) analizleri kullanılmıştır. SV, distile suda çözündüğünde BV'ye göre daha yüksek RSA (73.26±11.24%) sergilemiş, SV, askorbik asit (AA) ve Trolox RSA’ları arasında fark bulunmamıştır. BV'nin RSA'sı, AA (75.07±15.59%) ve Trolox (84.02±1.63%) RSA’larından düşük bulunmuştur. BV'nin MCA'sı (30.31±24.06%), AA (8.93±16.08%) değerini aşmıştır. SV, tuzlu su içinde çözüldüğünde BV'ye göre daha yüksek RSA (63.83±9.73%) sergilemiş, AA (71.63±4.14%) ve Trolox (79.01±6.94%) RSA'larından düşük bulunmuştur. SV (85.42±4.65%) ve BV (85.53±7.19%) örneklerinin MCA değerleri, Trolox (55.06±30.92%) değerini aşmıştır. SV (37.16±16.54%) ve BV (38.47±17.24%) örneklerinin PBS içindeki RSA değerleri arasında fark bulunmamış, her ikisi de AA (71.48±3.66%) ve Trolox (72.87±6.05%) değerlerinin altında kalmıştır. Optimal RSA ve MCA değerleri farklı çözücü ve konsantrasyonlarda gözlemlenmiş, bu durum 500 µg.ml-1 (BV için RSA'da 1.95 µg.ml-1) konsantrasyonda fizyolojik tuzlu su içinde çözünen zehirin optimal AOA için kullanılmasına işaret etmektedir. PBS veya distile su kullanımı ise AOA değerlerinde azalmaya neden olmuştur.

Keywords

Exploring Apis mellifera L. Venom's Antioxidant Power in Various Solvents: Unveiling its In Vitro Potential

Abstract

Apis mellifera L. venom contains bioactive components with antioxidant properties. Diluted in various polar solvents, the venom is utilized for therapeutic purposes. This study aims to determine the in vitro antioxidant activities (AOA) of standard crude venom (SV) and venom from breeders (BV) by dissolving them in distilled water, saline, and PBS at concentrations of 1.95-500 µg.ml-1. Radical scavenging activity (DPPH) and metal chelating activity (MCA) assays were employed for AOA assessment. SV dissolved in distilled water exhibited higher RSA (73.26±11.24%) than BV (34.60±21.08%), with no difference between SV, ascorbic acid (AA), and Trolox RSA’s. BV's RSA was lower than AA (75.07±15.59%) and Trolox (84.02±1.63%). BV's MCA (30.31±24.06%) exceeded AA (8.93±16.08%). SV in saline showed higher RSA (63.83±9.73%) than BV (46.99±18.31%), lower than AA (71.63±4.14%) and Trolox (79.01±6.94%). MCAs of SV (85.42±4.65%) and BV (85.53±7.19%) surpassed Trolox (55.06±30.92%). No difference existed between RSA’s of SV (37.16±16.54%) and BV (38.47±17.24%) in PBS, both lower than AA (71.48±3.66%) and Trolox (72.87±6.05%). Optimal RSA and MCA were observed at different solvents and concentrations, indicating the use of 500 µg.ml-1 (1.95 µg.ml-1 BV for RSA) venom dissolved in saline for optimal AOA. PBS or distilled water usage resulted in decreased AOA.

Keywords

Thanks

We would like to express our gratitude to Mr. Şevket Afyonlu, the beekeeper, for his valuable contribution in providing the honeybee specimens for this study.

References

Denk, B., & Fidan, A. F. (2021). Effects of honeybee (apis mellifera) venom on redox balance, biochemical andhematological profile in diabetic rats: A preliminary study. Turkish Journal of Veterinary & Animal Sciences, 45(2), 257-265.
Dontha, S. (2016). A review on antioxidant methods. Asian J. Pharm. Clin. Res, 9(2), 14-32. El Mehdi, I., Falcão, S. I., Boujraf, S., Mustapha, H., Campos, M. G., & Vilas-Boas, M. (2022). Analytical methods for honeybee venom characterization. Journal of Advanced Pharmaceutical Technology & Research, 13(3), 154.
Eze, O. B., Nwodo, O. F., & Ogugua, V. N. (2016). Therapeutic effect of honey bee venom. Proteins (enzymes), 1(2).
Frangieh, J., Salma, Y., Haddad, K., Mattei, C., Legros, C., Fajloun, Z., & El Obeid, D. (2019). First characterization of the venom from apis mellifera syriaca, a honeybee from the middle east region. Toxins, 11(4), 191.
Gulcin, İ., & Alwasel, S. H. (2022). Metal ions, metal chelators and metal chelating assay as antioxidant method. Processes, 10(1), 132.
Gupta, R. K., & Stangaciu, S. (2014). Apitherapy: holistic healing through the honeybee and bee products in countries with poor healthcare system. In Beekeeping for poverty alleviation and livelihood security (pp. 413-446). Springer, Dordrecht.
Han, S. M., Lee, G. G., & Park, K. K. (2012). Acute dermal toxicity study of bee venom (Apis mellifera L.) in rats. Toxicological research, 28, 99-102.
Hwang, D. S., Kim, S. K., & Bae, H. (2015). Therapeutic effects of bee venom on immunological and neurological diseases. Toxins, 7(7), 2413-2421.

Lee, K. S., Kim, B. Y., Yoon, H. J., Choi, Y. S., & Jin, B. R. (2016). Secapin, a bee venom peptide, exhibits anti-fibrinolytic, anti-elastolytic, and anti-microbial activities. Developmental & Comparative Immunology, 63, 27-35.
Martinello, M., & Mutinelli, F. (2021). Antioxidant activity in bee products: A review. Antioxidants, 10(1), 71.
Munteanu, I. G., & Apetrei, C. (2021). Analytical methods used in determining antioxidant activity: A review. International Journal of Molecular Sciences, 22(7), 3380.
Pinto, D., Vieira, E. F., Peixoto, A. F., Freire, C., Freitas, V., Costa, P., ... & Rodrigues, F. (2021). Optimizing the extraction of phenolic antioxidants from chestnut shells by subcritical water extraction using response surface methodology. Food Chemistry, 334, 127521.
Sobral, F., Sampaio, A., Falcão, S., Queiroz, M. J. R., Calhelha, R. C., Vilas-Boas, M., & Ferreira, I. C. (2016). Chemical characterization, antioxidant, anti-inflammatory and cytotoxic properties of bee venom collected in Northeast Portugal. Food and Chemical Toxicology, 94, 172-177.
Somwongin, S., Chantawannakul, P., & Chaiyana, W. (2018). Antioxidant activity and irritation property of venoms from Apis species. Toxicon, 145, 32-39.
Şenel, E., & Demir, E. (2018). Bibliometric analysis of apitherapy in complementary medicine literature between 1980 and 2016. Complementary therapies in clinical practice, 31, 47-52.
Zhang, S., Liu, Y., Ye, Y., Wang, X. R., Lin, L. T., Xiao, L. Y., ... & Liu, C. Z. (2018). Bee venom therapy: Potential mechanisms and therapeutic applications. Toxicon, 148, 64-73.

Details

Primary Language

English

Subjects

Veterinary Biochemistry

Journal Section

Research Article

Authors

Barış Denk ^*
0000-0002-7586-0895
Türkiye

Publication Date

September 30, 2023

Submission Date

August 14, 2023

Acceptance Date

September 25, 2023

Published in Issue

Year 2023 Volume: 16 Number: 3

DOI

https://doi.org/10.30607/kvj.1343130

IZ

https://izlik.org/JA88AR47NM

Cite

RIS / Bibtex

APA

Denk, B. (2023). Exploring Apis mellifera L. Venom’s Antioxidant Power in Various Solvents: Unveiling its In Vitro Potential. Kocatepe Veterinary Journal, 16(3), 420-431. https://doi.org/10.30607/kvj.1343130

AMA

1.Denk B. Exploring Apis mellifera L. Venom’s Antioxidant Power in Various Solvents: Unveiling its In Vitro Potential. kvj. 2023;16(3):420-431. doi:10.30607/kvj.1343130

Chicago

Denk, Barış. 2023. “Exploring Apis Mellifera L. Venom’s Antioxidant Power in Various Solvents: Unveiling Its In Vitro Potential”. Kocatepe Veterinary Journal 16 (3): 420-31. https://doi.org/10.30607/kvj.1343130.

EndNote

Denk B (September 1, 2023) Exploring Apis mellifera L. Venom’s Antioxidant Power in Various Solvents: Unveiling its In Vitro Potential. Kocatepe Veterinary Journal 16 3 420–431.

IEEE

[1]B. Denk, “Exploring Apis mellifera L. Venom’s Antioxidant Power in Various Solvents: Unveiling its In Vitro Potential”, kvj, vol. 16, no. 3, pp. 420–431, Sept. 2023, doi: 10.30607/kvj.1343130.

ISNAD

Denk, Barış. “Exploring Apis Mellifera L. Venom’s Antioxidant Power in Various Solvents: Unveiling Its In Vitro Potential”. Kocatepe Veterinary Journal 16/3 (September 1, 2023): 420-431. https://doi.org/10.30607/kvj.1343130.

JAMA

1.Denk B. Exploring Apis mellifera L. Venom’s Antioxidant Power in Various Solvents: Unveiling its In Vitro Potential. kvj. 2023;16:420–431.

MLA

Denk, Barış. “Exploring Apis Mellifera L. Venom’s Antioxidant Power in Various Solvents: Unveiling Its In Vitro Potential”. Kocatepe Veterinary Journal, vol. 16, no. 3, Sept. 2023, pp. 420-31, doi:10.30607/kvj.1343130.

Vancouver

1.Barış Denk. Exploring Apis mellifera L. Venom’s Antioxidant Power in Various Solvents: Unveiling its In Vitro Potential. kvj. 2023 Sep. 1;16(3):420-31. doi:10.30607/kvj.1343130

Cited By

Exploring the therapeutic potential of honeybee venom in mitigating diabetic reproductive complications: insights from in vitro and in vivo studies in rats

Archives of Veterinary Science

https://doi.org/10.5380/avs.v31i1.101661

Therapeutic Potential of Bee Venom and Capsicum annum Hydroethanolic Extract in Experimental Model of Rheumatoid Arthritis in Male Rats

Mediators of Inflammation

https://doi.org/10.1155/mi/5550872

Algerian honeybee venom antioxidant and antimicrobial activities: molecular docking of melittin as a dual inhibitor of PBP2a and Keap1

Frontiers in Food Science and Technology

https://doi.org/10.3389/frfst.2026.1919918