In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş

Osman Yiğit; Adem Bardak

doi:10.34248/bsengineering.2015174

TR EN

In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş

Abstract

Olive tree (Olea europaea L.) is one of the oldest known and cultivated trees in the world. It is cultivated in many regions owing to its high economic value, high nutrient and oil content, drought resistance, and ability to produce fruit for many years. However, since vegetative propagation, particularly by cuttings, is difficult for many olive cultivars, classical propagation methods remain limited. In this study, in vitro micropropagation of a local oil-type olive genotype grown in the Kahramanmaraş region was investigated using explants of that genotype. Explants were first surface-sterilized and then transferred to ½ MS medium. Shoots proliferating on this medium were subsequently transferred to a regeneration medium to determine the most suitable medium for propagation. Four different media (MS, DKW, OM, and WPM), each supplemented with 1 mg/L of the plant growth regulator BAP (6-benzylaminopurine), were tested for shoot regeneration. Rooting trials were then established on the best-performing regeneration medium, to which different concentrations of IBA (0.5, 1.0, and 1.5 mg/L) were added. The best surface sterilization result was obtained with 15% NaOCl combined with a 2 h chlorine dioxide pre-treatment, giving the highest viability (88.9%). Among the four media tested, WPM supplemented with 1 mg/L BAP gave the best results (shoot length: 16.39 mm; callus formation: 100%), and 1.0 mg/L IBA gave the most effective rooting response among the concentrations tested (shoot length: 15.58 mm).

Keywords

Ethical Statement

Ethics committee approval was not required for this study because of there was no study on animals or humans.

Thanks

This article was derived from the master's thesis of Osman Yiğit, entitled "In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş," prepared under the supervision of Adem Bardak at the Graduate School of Natural and Applied Sciences, Kahramanmaraş Sütçü İmam University.

In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş

Abstract

Olive tree (Olea europaea L.) is one of the oldest known and cultivated trees in the world. It is cultivated in many regions owing to its high economic value, high nutrient and oil content, drought resistance, and ability to produce fruit for many years. However, since vegetative propagation, particularly by cuttings, is difficult for many olive cultivars, classical propagation methods remain limited. In this study, in vitro micropropagation of a local oil-type olive genotype grown in the Kahramanmaraş region was investigated using explants of that genotype. Explants were first surface-sterilized and then transferred to ½ MS medium. Shoots proliferating on this medium were subsequently transferred to a regeneration medium to determine the most suitable medium for propagation. Four different media (MS, DKW, OM, and WPM), each supplemented with 1 mg/L of the plant growth regulator BAP (6-benzylaminopurine), were tested for shoot regeneration. Rooting trials were then established on the best-performing regeneration medium, to which different concentrations of IBA (0.5, 1.0, and 1.5 mg/L) were added. The best surface sterilization result was obtained with 15% NaOCl combined with a 2 h chlorine dioxide pre-treatment, giving the highest viability (88.9%). Among the four media tested, WPM supplemented with 1 mg/L BAP gave the best results (shoot length: 16.39 mm; callus formation: 100%), and 1.0 mg/L IBA gave the most effective rooting response among the concentrations tested (shoot length: 15.58 mm).

Keywords

Ethical Statement

Ethics committee approval was not required for this study because of there was no study on animals or humans.

Thanks

This article was derived from the master's thesis of Osman Yiğit, entitled "In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş," prepared under the supervision of Adem Bardak at the Graduate School of Natural and Applied Sciences, Kahramanmaraş Sütçü İmam University.

References

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Details

Primary Language

English

Subjects

Plant Biotechnology

Journal Section

Research Article

Authors

Osman Yiğit
0000-0001-5351-0563
Türkiye

Adem Bardak ^*
0000-0002-5715-302X
Türkiye

Publication Date

November 15, 2026

Submission Date

August 10, 2026

Acceptance Date

September 16, 2026

Published in Issue

Year 2026 Volume: 9 Number: 6

DOI

https://doi.org/10.34248/bsengineering.2015174

IZ

https://izlik.org/JA29FX48LK

Cite

RIS / Bibtex

APA

Yiğit, O., & Bardak, A. (2026). In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş. Black Sea Journal of Engineering and Science, 9(6), 3051-3057. https://doi.org/10.34248/bsengineering.2015174

AMA

1.Yiğit O, Bardak A. In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş. BSJ Eng. Sci. 2026;9(6):3051-3057. doi:10.34248/bsengineering.2015174

Chicago

Yiğit, Osman, and Adem Bardak. 2026. “In Vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea Europaea L.) from Kahramanmaraş”. Black Sea Journal of Engineering and Science 9 (6): 3051-57. https://doi.org/10.34248/bsengineering.2015174.

EndNote

Yiğit O, Bardak A (November 15, 2026) In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş. Black Sea Journal of Engineering and Science 9 6 3051–3057.

IEEE

[1]O. Yiğit and A. Bardak, “In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş”, BSJ Eng. Sci., vol. 9, no. 6, pp. 3051–3057, Nov. 2026, doi: 10.34248/bsengineering.2015174.

ISNAD

Yiğit, Osman - Bardak, Adem. “In Vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea Europaea L.) from Kahramanmaraş”. Black Sea Journal of Engineering and Science 9/6 (November 15, 2026): 3051-3057. https://doi.org/10.34248/bsengineering.2015174.

JAMA

1.Yiğit O, Bardak A. In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş. BSJ Eng. Sci. 2026;9:3051–3057.

MLA

Yiğit, Osman, and Adem Bardak. “In Vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea Europaea L.) from Kahramanmaraş”. Black Sea Journal of Engineering and Science, vol. 9, no. 6, Nov. 2026, pp. 3051-7, doi:10.34248/bsengineering.2015174.

Vancouver

1.Osman Yiğit, Adem Bardak. In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş. BSJ Eng. Sci. 2026 Nov. 15;9(6):3051-7. doi:10.34248/bsengineering.2015174